[PDF] Top 20 Multicenter Evaluation of Methods To Quantitate Human Immunodeficiency Virus Type 1 RNA in Seminal Plasma
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Multicenter Evaluation of Methods To Quantitate Human Immunodeficiency Virus Type 1 RNA in Seminal Plasma
... the evaluation at one or more ...viral RNA extraction (2) and four laboratories (B, D, E, and F) did not have previous experience with viral RNA measurements from seminal plasma ... See full document
6
Determining Seminal Plasma Human Immunodeficiency Virus Type 1 Load in the Context of Efficient Highly Active Antiretroviral Therapy
... 1,500 RNA cop- ies/ml) that indicate a real risk of transmission (6, ...200 RNA copies/ml, the risks of infection and HIV transmission are probably very low and not significantly different than those for ... See full document
5
Use of Multiple Competitors for Quantification of Human Immunodeficiency Virus Type 1 RNA in Plasma
... of RNA competitors with different copy numbers covering the common interval in HIV-1 viremias (19) were ...of RNA competitors were designed to have a minor overlap in copy ...HIV-1 RNA ... See full document
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Clinical Evaluation of an In House Reverse Transcription Competitive PCR for Quantitation of Human Immunodeficiency Virus Type 1 RNA in Plasma
... an evaluation of any in-house proce- ...of plasma samples indicated that the results obtained by the in-house assay and the commercial AMPLICOR HIV-1 MONITOR test are in good ...HIV-1 ... See full document
6
Ultrasensitive Reverse Transcription PCR Assay for Quantitation of Human Immunodeficiency Virus Type 1 RNA in Plasma
... Specimens processed by both the standard and ultrasensitive methods were amplified on a GeneAmp PCR system 9600 thermal cycler (Perkin-Elmer Cor- poration, Norwalk, Conn.) as described in the AMPLICOR HIV-1 ... See full document
6
Highly Sensitive Methods for Quantitation of Human Immunodeficiency Virus Type 1 RNA from Plasma, Cells, and Tissues
... viral RNA in specimens from human immunodeficiency virus (HIV) type 1 (HIV-1)-infected individuals has become an indispensable tool for the monitoring of the efficacy of ... See full document
5
Multicenter Evaluation of the Performance Characteristics of the NucliSens HIV 1 QT Assay Used for Quantitation of Human Immunodeficiency Virus Type 1 RNA
... Currently, HIV-1 viral load assays are not FDA approved for the diagnosis of HIV disease. Despite this fact, in clinical practice the assays have been used “off label” to identify per- sons with acute HIV-1 ... See full document
10
Genotypic Changes in Human Immunodeficiency Virus Type 1 Associated with Loss of Suppression of Plasma Viral RNA Levels in Subjects Treated with Ritonavir (Norvir) Monotherapy
... resistant virus to drop to ...by plasma HIV RNA levels, was corre- lated with plasma RNA levels at both baseline and the nadir ...that evaluation of AUC or C min may result in a ... See full document
11
Comparative evaluation of NASBA HIV 1 RNA QT, AMPLICOR HIV monitor, and QUANTIPLEX HIV RNA assay, three methods for quantification of human immunodeficiency virus type 1 RNA in plasma
... quantifying plasma human immunodeficiency virus type 1 (HIV-1) RNA were ...and methods of amplification and detection. Plasma samples were obtained ... See full document
7
Multicenter Evaluation of the New Abbott RealTime Assays for Quantitative Detection of Human Immunodeficiency Virus Type 1 and Hepatitis C Virus RNA
... C virus (HCV) and human immunode- ficiency virus type 1 viral load assays were compared at nine laboratories with different competitor ...pre-m1000 RNA isolation spin were ... See full document
6
Design and Evaluation of a Human Immunodeficiency Virus Type 1 RNA Assay Using Nucleic Acid Sequence Based Amplification Technology Able To Quantify Both Group M and O Viruses by Using the Long Terminal Repeat as Target
... HIV-1 RNA by hybridization with labeled probes ...of RNA per ml of plasma or serum (5, 44), with an input of 1,000 or 200 ml, ...60 RNA copies per ml of plasma when an ... See full document
6
Correlation between Human Immunodeficiency Virus Type 1 (HIV 1) RNA Measurements Obtained with Dried Blood Spots and Those Obtained with Plasma by Use of Nuclisens EasyQ HIV 1 and Abbott RealTime HIV Load Tests
... tested by the m2000rt assay were better than the results ob- tained in our study. It should be noted, however, that nucleic acid isolation was performed automatically in their study, while we used a manual protocol. ... See full document
6
Multicenter Performance Evaluation of a New TaqMan PCR Assay for Monitoring Human Immunodeficiency Virus RNA Load
... TaqMan human immunodeficiency virus (HIV) (HPS-CTM HIV) PCR assay, recently developed for the quantification of HIV type 1 RNA in plasma, was evaluated in comparison with ... See full document
5
Multicenter comparison of three commercial methods for quantification of human immunodeficiency virus type 1 RNA in plasma
... of human immunodeficiency virus type 1 (HIV-1) RNA from plasma were compared at three ...HIV-1-infected human plasma samples, cross-sectionally ... See full document
7
Evaluation of a quality assurance program for quantitation of human immunodeficiency virus type 1 RNA in plasma by the AIDS Clinical Trials Group virology laboratories
... performance evaluation in the present ...patient plasma which would influence assay ...HIV-1 RNA concentration in patient samples have been addressed with a small number of patients (27, ... See full document
7
Quantification of human immunodeficiency virus type 1 RNA from dried plasma spots collected on filter paper
... HIV-1 RNA on filter paper has far-reaching epidemiological and public health im- ...of RNA levels with DPSs will be particularly valuable in the neonatal setting, in which only minute amounts of ... See full document
7
Quantitative Detection of Plasma Human Immunodeficiency Virus Type 2 Subtype A RNA by the Nuclisens EasyQ Assay (Version 1 1)
... this evaluation provide acceptable confidence for the use of the Nuclisens EasyQ assay in clinical settings to monitor plasma viremia in HIV-2-infected ...HIV-2 RNA copies estimated in an HIV-2 viral ... See full document
5
Human Seminal Plasma Abrogates the Capture and Transmission of Human Immunodeficiency Virus Type 1 to CD4+ T Cells Mediated by DC-SIGN
... Human SP contains a diverse array of components, including lipids, carbohydrates, peptides, proteins, cytokines, and che- mokines. These components are secreted from different sourc- es: the testis, epididymis, ... See full document
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Nucleic Acid Sequence Based Amplification (Nasba)-Prospects And Applications
... HIV-1 RNA QT, ...HIV-1 RNA between 25 and >5×10 6 copies/mL and has a 95% detection rate of 176 copies/mL, using 1 mL of EDTA, citrate, or heparin plasma (Notermans et ... See full document
16
Multicenter Quality Control for the Detection of Hepatitis C Virus RNA in Seminal Plasma Specimens
... C virus (HCV) RNA in seminal plasma of men chronically infected by this agent are related, at least in part, to the molecular techniques used and particularly to the wide range of protocols ... See full document
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