[PDF] Top 20 Multiplex PCR Protocol for the Diagnosis of Staphylococcal Infection
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Multiplex PCR Protocol for the Diagnosis of Staphylococcal Infection
... by PCR would delay treatment with a preferred class of drugs, the delay would not extend beyond the time frame associated with conventional diagnostic methods, since treatment could be modified as soon as ... See full document
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Multiplex Immunoassay for Serological Diagnosis of Mycobacterium bovis Infection in Cattle
... Preparation of antigens. The genes encoding different TB proteins were ex- pressed in Escherichia coli as N-terminal polyhistidine-tagged (6 ⫻ His) fusion proteins by Fusion Antibodies Ltd. (Belfast) using the patented ... See full document
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Multiplex Amplification Refractory Mutation System Polymerase Chain Reaction (ARMS PCR) for diagnosis of natural infection with canine distemper virus
... CDV infection mainly relies on the use of live attenuated ...in infection as the mod- ified live vaccines may result in a false positive in the first few weeks after ...clinical diagnosis as a ... See full document
9
Sensitive and Specific Detection of Staphylococcal Epidermolysins A and B in Broth Cultures by Flow Cytometry Assisted Multiplex Immunoassay
... form, staphylococcal scalded skin ...cytometry-assisted multiplex immunoassay (Bio-Plex system) for the detection of ETA and ...a PCR-based ... See full document
5
Comparison of three diagnostic methods (microscopy, RDT, and PCR) for the detection of malaria parasites in representative samples from Equatorial Guinea
... mixed infection in ...accurate diagnosis followed by effective treatment. Early and accurate diagnosis of malaria is essential for both effective disease management and malaria ...malaria ... See full document
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Multiplex pcr based detection of leptospira canicola in environmental water samples obtained from nagapattinam area
... a protocol based on multiplex PCR, which is currently used to detect leptospiral DNA in clinical samples, for environmental water samples using two sets of primers that allow the differentiation of ... See full document
5
DNA Differential Diagnosis of Taeniasis and Cysticercosis by Multiplex PCR
... Taenia solium, Taenia saginata, and Taenia asiatica are known as causative agents of taeniasis in humans. T. solium also causes cysticercosis in humans. In particular, neurocysticercosis caused by larval T. solium ... See full document
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Improved Diagnosis of Periprosthetic Joint Infection by Multiplex PCR of Sonication Fluid from Removed Implants
... rDNA) PCR was applied to synovial fluid or periprosthetic tissue (3–7, 9, 14, 17, 18, 26, ...specific PCR for detection of ...broad-range PCR of tissue cultures of patients with PJI showed a ... See full document
7
Comparison of Multiplex PCR-ELISA and conventional Multiplex PCR for detection of HIV-1/HCV co-infection
... rapid diagnosis of life threaten- ing infectious agents (1, ...employ multiplex-PCR techniques or reverse transcription (RT)- polymerase chain reaction (PCR) for detection of several ... See full document
6
Multiplex PCR To Diagnose Bloodstream Infections in Patients Admitted from the Emergency Department with Sepsis
... the diagnosis of bloodstream in- ...real-time multiplex PCR assay as an adjunct to blood culture for the rapid identification of bloodstream ... See full document
8
Detection of Staphylococcus aureus Enterotoxins A to D by Real Time Fluorescence PCR Assay
... secreted staphylococcal virulence factors, there is a growing list of enterotoxins which can induce gastroenteric syndrome and toxic shock ...fluorescence PCR assay (TaqMan PCR) for the detection of ... See full document
5
Multiplex PCR Method for Species Identification of Coagulase Positive Staphylococci
... the staphylococcal nuc genes, which were available from NCBI ...the PCR consisted of 2 l of DNA extract in a total volume of 50 l composed of 2 U of Ex Taq (Takara ...The PCR product was cloned ... See full document
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Development of a Multiplex PCR Ligase Detection Reaction Assay for Diagnosis of Infection by the Four Parasite Species Causing Malaria in Humans
... in PCR primer and LDR probe annealing sites can influence DNA base pairing and the efficiency of amplification or probe hy- bridization and, therefore, the intensities of the species-specific LDR band signal ... See full document
8
Multiplex PCR analysis of clusters of unexplained viral respiratory tract infection in Cambodia
... Between July 25 th and September 12 th of 2011 a cluster of 11 children less than five years of age from Ratanakiri received a diagnosis of pneumonia. Ten of them had shortness of breath; eight had nausea and ... See full document
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Rapid Diagnosis of Leptospirosis by Multiplex PCR
... Using an in-house genomic extraction protocol, DNA extraction was carried out on the cell pellets of leptospiral and bacterial cultures. Briefly, 1.5 mL of culture isolate was centrifuged at 13 000 g for 5 minutes ... See full document
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Detection of sexually transmitted infection and human papillomavirus in negative cytology by multiplex PCR
... A total of 235 married and sexually active women (mean age of 37.9 y; ages range 21 to 48 years) were recruited by the Department of cytopathology of Yonsei Univer- sity College of Medicine for this study. Specimens col- ... See full document
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Detection of and Discrimination between Gram Positive and Gram Negative Bacteria in Intraocular Samples by Using Nested PCR
... nested PCR protocol has been developed for the detection of and discrimination between 14 species of gram-positive and -negative bacteria in samples of ocular ...First-round PCR with pan-bacterial ... See full document
5
PCR Based Diagnosis of Prosthetic Joint Infection
... prosthesis infection, septic loosening, aseptic loosening, replacement, or arthroplasty and (ii) ...of PCR for the diagnosis of joint infection in comparison with visible purulence of joint ... See full document
5
NEONATAL STAPHYLOCOCCAL INFECTION
... STAPHYLOCOCCAL DISEASE DATA EXPERiMENTAL GROUP. Reg, No[r] ... See full document
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Detection and identification of mycobacteria in sputum from suspected tuberculosis patients
... in multiplex PCR reverse cross blot hybridization assay, 149 samples gave positive results with the probes pMyc5a, pt3 and pTub1 except 13 samples were found negative for the probe pMyc5a and pPt3 and the ... See full document
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