[PDF] Top 20 Optimization of recombinant human transferrin expression in insect cells baculovirus system
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Optimization of recombinant human transferrin expression in insect cells baculovirus system
... Studies of proteins for the development of drug therapies, vaccines, and insights into biological function depend upon the ability to produce large amounts of structurally complex proteins. It is important that these ... See full document
45
Cloning and Expression of Simian Rotavirus Spike Protein (VP4) in Insect Cells by Baculovirus Expression System
... the Baculovirus Autographa californica nuclear polyhedrosis virus (AcNPV) downstream of the strong polyhedrin ...The recombinant AcNPV-VP4 DNA was transfected by lipofection into the insect cell ... See full document
10
PR Domain of Rous Sarcoma Virus Gag Causes an Assembly/Budding Defect in Insect Cells
... overexpression system, in effect preventing proper ...in insect cells, we created three recombinant baculoviruses ...second recombinant bac- ulovirus, ⌬PR, expresses an RSV Gag mutant ... See full document
6
Optimization of canine interleukin 12 production using a baculovirus insect cell expression system
... To determine the quality, samples of the purified pro- tein were analyzed by SDS–PAGE, Western blot, fluo- rescence emission spectrum, circular dichroism, and for endotoxin contamination. Evaluation carried out by ... See full document
11
Expression and characterization of hepatitis B virus surface antigen polypeptides in insect cells with a baculovirus expression system.
... The expression of two S polypeptides by recombinant viruses 373S and 106FS suggested that the polypeptides represented the unglycosylated p25 and glycosylated gp29 forms of the small HBs[r] ... See full document
9
Expression of Recombinant Small Hydrophobic Protein for Serospecific Detection of Avian Pneumovirus Subgroup C
... The baculovirus expression system has been used extensively to express large quantities of proteins that are antigenically similar to their native counterparts and can be used in stan- dardized ... See full document
5
The effect of time and optimization of the baculovirus expression system for more efficient recombinant protein production of porcine epidemic diarrhea virus in insect cells
... titer baculovirus stock also contains fetal calf serum (FCS) from the growth medium and catabolic side products of cell metabolism, which may show toxic e ects on the insect ...serum-free insect cell ... See full document
9
Expression of the Herpes Simplex Virus Type 2 Glycoprotein D in Baculovirus Expression System and Evaluation of Its Immunogenicity in Guinea Pigs
... Bac-to-Bac expression system was used to express HSV-2 gD in insect ...the recombinant gD2 could elicit humoral responses in guinea pigs as measured by neutralization test and ELISA, and ... See full document
8
Characterization of Neospora caninum Surface Protein NcSRS2 Based on Baculovirus Expression System and Its Application for Serodiagnosis of NeosporaInfection
... the recombinant NcSRS2 expressed by Ba/SRS2p44 was observed on the surface of the infected cells (data not ...of recombinant NcSRS2 was detected in infected cells and that the mature truncated ... See full document
5
Development of novel galactosylation method for the expression of recombinant human transferrin in insect cell culture
... cultured cells can be controlled by various factors, which are sugar acceptor as model protein, substrate donor also known as sugar nucleotide and glycosyltransferase as ...that insect cells contain ... See full document
37
Expression and characterization of the human erythrocyte glucose transporter in insect cells using a recombinant baculovirus
... the cells blotted onto a ...putative recombinant-containing agarose plugs are picked and subjected to further screening and ...the cells tend to smear during blotting, enlarging the apparent diameter ... See full document
325
Evaluation of an Enzyme Linked Immunosorbent Assay with Recombinant Rhoptry Associated Protein 1 Antigen against Babesia bovis for the Detection of Specific Antibodies in Cattle
... coli expression vector pGEMEX-2 (Promega ...The recombinant donor plasmid, pFB/RAP-1-Ht, was transformed into DH10Bac competent cells (Life ...cotransfect insect (Spodoptera frugiperda) ... See full document
5
Characterization of Toxoplasma gondii SAG2 Expressed in Insect Cells by Recombinant Baculovirus and Evaluation of Its Diagnostic Potential in an Enzyme-Linked Immunosorbent Assay
... a recombinant baculovirus carrying the gene encoding SAG2 of ...Sf9 cells do not recognize the GPI attachment site and degraded the full-length molecule ... See full document
5
Analysis of Antigenicity and Topology of E2 Glycoprotein Present on Recombinant Hepatitis C Virus-Like Particles
... vitro expression studies have shown that both glycoproteins associate to form two types of com- plexes: (i) a heterodimer stabilized by noncovalent interactions and (ii) high-molecular-weight disulfide-linked ... See full document
12
Expression and self-assembly of empty virus-like particles of hepatitis E virus.
... Nevertheless, the formation of the VLPs described here is very important for seroepidemiological studies. The VLPs ap- peared to retain the antigenicity of the native virion, as dem- onstrated by IEM with serum samples ... See full document
7
High-Level Expression of Truncated Surface Antigen P50 of Babesia gibsoni in Insect Cells by Baculovirus and Evaluation of Its Immunogenicity and Antigenicity
... from insect cells or ...of insect cells infected with recombinant baculoviruses and, therefore, provide an easy way to prepare pure immuno- genic proteins ...in insect ... See full document
6
Properties of the protein encoded by the UL32 open reading frame of herpes simplex virus 1.
... to human growth ...with human growth hormone-binding ...between human growth hormone and its binding pro- tein, immobilized phage could be released only by protease cleavage at the random peptide ... See full document
9
The UL8 subunit of the herpes simplex virus helicase-primase complex is required for efficient primer utilization.
... We purified both the three-subunit helicase-primase complex and a UL5/UL52 subcomplex from recombinant baculovirus-infected insect cells and showed that the specific activities of the tw[r] ... See full document
9
Identification and Expression of a 50 Kilodalton Surface Antigen of Babesia gibsoni and Evaluation of Its Diagnostic Potential in an Enzyme Linked Immunosorbent Assay
... cDNA expression library prepared from Babesia gibsoni merozoite mRNA was screened with ...The recombinant P50 protein expressed by baculovirus in insect cells was used as the antigen in ... See full document
7
Assembly of double-shelled, viruslike particles of bluetongue virus by the simultaneous expression of four structural proteins.
... Coexpression of the two major core proteins VP3 and VP7 in insect cells by a multiple recombinant baculovirus results in the synthesis of CLPs of the same size, appearance, and stoichiom[r] ... See full document
6
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