[PDF] Top 20 Orthoretroviral like prototype foamy virus gag pol expression is compatible with viral replication
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Orthoretroviral like prototype foamy virus gag pol expression is compatible with viral replication
... PFV Pol protein, that appear to have no proteo- lytic, but polymerase, ...of Pol secretion remains unclear. Furthermore, we demon- strate that PFV Pol translation in an ... See full document
14
Human foamy virus reverse transcription that occurs late in the viral replication cycle.
... unintegrated virus DNAs result solely from superinfection, we analyzed the occur- rence of virus cDNA of the so-called human FV isolate (HFV) in cells transfected with a virus mutant deficient in the ... See full document
7
A nuclear export signal within the structural Gag protein is required for prototype foamy virus replication
... PFV Gag proteins could be involved in this critical step, in a way similar to what was reported for RSV ...PFV Gag proteins would bridge the nuclear intron-con- taining viral RNAs thanks to the GRI ... See full document
11
Foamy Virus Pol Protein Expressed as a Gag-Pol Fusion Retains Enzymatic Activities, Allowing for Infectious Virus Production
... the expression of Gag-Pol proteins that contain cleavage sites in both Gag and Pol led to the production of virions with a titer similar to the WT (G-P in Table ...PFV ... See full document
10
The cooperative function of arginine residues in the Prototype Foamy Virus Gag C terminus mediates viral and cellular RNA encapsidation
... packaging, Pol encapsidation, capsid assembly, and as previously shown RTr in FV ...PFV Gag protein mu- tant deficient in vgRNA encapsidation independent of the virus production system ...that ... See full document
17
Correct Capsid Assembly Mediated by a Conserved YXXLGL Motif in Prototype Foamy Virus Gag Is Essential for Infectivity and Reverse Transcription of the Viral Genome
... PFV Gag L3 mutant revealed aberrant particle morphogenesis ...with expression vec- tors for PFV Env, PFV Pol, a packageable vector RNA, and selected PFV Gag point mutants were examined by ... See full document
10
Prototype Foamy Virus Envelope Glycoprotein Leader Peptide Processing Is Mediated by a Furin-Like Cellular Protease, but Cleavage Is Not Essential for Viral Infectivity
... Pol-expressing, replication-defective retroviral vector pczDWP001 and the respective PFV Env expression construct as ...corresponding viral particle lysates; mock, lysate from cells ... See full document
6
Novel Functions of Prototype Foamy Virus Gag Glycine- Arginine-Rich Boxes in Reverse Transcription and Particle Morphogenesis
... abolish Pol packaging despite strong overexpression of Pol in the transfected cells of a ⌬GRI mutant ...a replication-deficient four-plasmid expression-opti- mized vector system, Pol ... See full document
12
The Roles of Pol and Env in the Assembly Pathway of Human Foamy Virus
... the Pol protein or the Env protein, we demonstrate here that HFV assembly is ...Transient expression of proviral HFV pol and env mutants in FAB cells has permitted us to evaluate the roles of ... See full document
8
Subviral Particle Release Determinants of Prototype Foamy Virus
... machinery. Like HBV and a variety of other viruses, PFV hijacks the cellular VPS machinery for viral particle ...the Gag protein and the ESCRT-I component TSG101 (24, 38, 48, ...HBV viral ... See full document
12
A purine rich element in foamy virus pol regulates env splicing and gag/pol expression
... for gag expression, we analysed two deletion mutants where the SpeI or the SexAI fragments of pHSRV13 harbouring the C element and known env 3 ′ ss had been removed ...expressed gag in similar ... See full document
11
Role of the C Terminus of Foamy Virus Gag in RNA Packaging and Pol Expression
... decreased Pol expression could be that the C terminus of FV Gag is involved in the proper transcription and/or translation of ...of Pol translation, although there is currently no experimental ... See full document
8
The human foamy virus pol gene is expressed as a Pro-Pol polyprotein and not as a Gag-Pol fusion protein.
... human foamy virus (HFV) Pol polyprotein of 120 kDa is synthesized in the absence of the active HFV aspartic ...HFV pol expression and ...of gag and thus lack a ... See full document
8
Determination of the relative amounts of Gag and Pol proteins in foamy virus particles
... relative Gag to Pol ...for Pol protein incorporation into FV particles [4]? Firstly, with respect to this study only the minimal RNA sequence requirements for Pol protein encapsidation using ... See full document
7
Incorporation of Pol into Human Immunodeficiency Virus Type 1 Gag Virus-Like Particles Occurs Independently of the Upstream Gag Domain in Gag-Pol
... for Pol incorporation into human immunodeficiency virus type 1 (HIV-1) Gag virus-like particles (VLPs), it has been found that truncated, protease-negative, Gag-Pol ... See full document
8
Association of Murine Leukemia Virus Pol with Virions, Independent of Gag-Pol Expression
... infectious virus from cells, might be ...specific Gag and Pol interactions are occurring, to define their roles in virion for- mation, and to identify potential intermolecular PR or C-ter- minal NC ... See full document
6
Prototype Foamy Virus Gag Nuclear Localization: a Novel Pathway among Retroviruses
... PFV Gag remain ...the viral replication cycle the individual motifs exert their ...(AFP)-tagged Gag (and mutants thereof) (50), which we analyzed using a live-cell fluorescence microscopy ... See full document
10
Expression and maturation of human foamy virus Gag precursor polypeptides.
... the Gag polypeptides of human foamy virus previously reported to be ...free virus particles, two Gag precursor polypeptides were identified at approximately 72 and 68 kDa, p72 giving ... See full document
5
The R Region Found in the Human Foamy Virus Long Terminal Repeat Is Critical for both Gag and Pol Protein Expression
... Gag expression can be largely rescued by the introduction of an intron into the 5 ⴕ noncoding sequence in place of the R region but not by an intron or any one of several distinct retroviral nuclear RNA ... See full document
8
An N terminal domain helical motif of Prototype Foamy Virus Gag with dual functions essential for particle egress and viral infectivity
... within Gag is less well ...additional Gag interaction determinants of the prototype FV (PFV) isolate using a combination of particle release, GST pull-down and single cycle infectivity analysis ... See full document
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