[PDF] Top 20 Phenotypic screening with 3D cell-based assays
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Phenotypic screening with 3D cell-based assays
... the screening results combining the first principal component, PC0, for both EGF- and HGF-induced phenotypic changes in a single ...the phenotypic training using HGF treated and untreated samples ... See full document
201
Airway remodeling disease: primary human structural cells and phenotypic and pathway assays to identify targets with potential to prevent or reverse remodeling
... higher-throughput screening in single, relevant cell types, followed by more relevant multi-cell and matrix ...high-throughput screening. These phenotypic assays are compatible ... See full document
11
Yeast-based assays for screening 11β-HSD1 inhibitors
... 250 μl of yeast culture volume were centrifuged and re- suspended in 500 μl 1 × PBS. Flow cytometric analyses were performed using the FACSCalibur instrument. The instrument settings were as follows: log forward scatter ... See full document
13
Mechanically produced schistosomula as a higher-throughput tools for phenotypic pre-screening in drug sensitivity assays: current research and future trends
... Despite the wide application of bright-field, light micro- scopic assessment of schistosome viability, this technique presents several problems. Firstly, the personnel should be well trained to acquire sufficient ... See full document
18
The 3D Cell Culture System in the Study of Tumor Applications and Prospects
... the 3D interaction between solid tumor cells and the matrix ...universal 3D cancer culture and screening device to ensure a high degree of flexibility in selecting well-developed 3D culture ... See full document
8
Development of a 3D Tissue Culture-Based High-Content Screening Platform That Uses Phenotypic Profiling to Discriminate Selective Inhibitors of Receptor Tyrosine Kinases.
... of cell-gel mix was added to each well of a 384-well plate using a CyBi Selma 96/60 robotic liquid handler (Analytik Jena AG, Jena, Germany), at a final cell density of 2000 cells per ... See full document
11
Target Mechanism Based Whole Cell Screening Identifies Bortezomib as an Inhibitor of Caseinolytic Protease in Mycobacteria
... enables screening for compounds that affect the pathway of ...promoter assays provide means to identify hits that are whole-cell active and pathway spe- cific but may not provide information on the ... See full document
13
Genetic toxicity assessment of engineered nanoparticles using a 3D in vitro skin model (EpiDerm™)
... 2D assays conducted in this study on the basis that they are recommended in the OECD test guideline for the in vitro micronucleus assay ...is based upon: their human origin ...other cell lines [21, ... See full document
21
Rescuing compounds for Lesch-Nyhan disease identified using stem cell–based phenotypic screening
... these cell types are fully amenable to high-throughput drug screening ...different cell types to mobilize alternative energy supplies to cope with bioenergetics stress — a property termed “metabolic ... See full document
16
Screening for Drought Tolerance in Tepary and Common Bean Based on Osmotic Potential Assays
... The genetic resources of common bean and tepary bean available in Mexico are abundant but so far underused, mainly because of lack of phenotypic/genotypic characterization [16]. Tepary bean is a crop adapted to ... See full document
9
Original Article MicroRNA-223-3p functions as a tumor suppressor by regulating cell cycle in hepatocellular carcinoma
... To address the molecular mechanisms involved in miR-223-3p in inhibition of proliferation and invasion, we searched for potential target ge- nes with an established or potential function in HCC. Interestingly, a perfect ... See full document
10
A method to enhance the hit ratio by a combination of structure-based drug screening and ligand-based screening
... silico screening methods based on the protein- compound affi nity matrix (Fukunishi et al 2005a, 2005b, 2006a, 2006b, 2006c, 2006d, ...One screening method is a structure-based screening ... See full document
10
Small molecule inhibition of Axl receptor tyrosine kinase potently suppresses multiple malignant properties of glioma cells
... II) are well differentiated and resemble normal tissue, whereas higher grade tumours (grade III and grade IV) are anaplastic and display high cell atypia and mitotic activity [15]. Glioblastoma multiforme (GBM) is ... See full document
15
High throughput spheroid screens using volume, resazurin reduction and acid phosphatase activity
... dimensional (3D) aggregates of cells displaying physiologically relevant gradients of oxygen, nutrients, cell-cell and cell matrix interactions (2, 3). Aggregate cultures were first described ... See full document
23
Accuracies of Leuconostocphenotypic identification: a comparison of API systems and conventional phenotypic assays
... criteria and either or both of the genotypic assays (PCR and 16S rDNA sequence analysis). As we suspected that some isolates might have been Lactobacillus, also a lactic acid bacteria with overlapping phenotypes, ... See full document
8
Comparison of 11 Phenotypic Assays for Accurate Detection of Carbapenemase Producing Enterobacteriaceae
... the assays, ease of use, necessary equipment, time to perform the tests, and turnaround time should all be considered when determining which carbapenemase assay is the most appropriate to incorporate into a ... See full document
10
Original Article miR-506, downregulated in glioma, inhibits cell growth and disrupts the cell cycle by regulating YAP
... Initially defined in Drosophilia, the Hippo path- way and its regulatory target, YAP, has recently emerged as an important biochemical signaling pathway, the function of which mainly includes regulation of organ size, ... See full document
11
Phytochemical screening and comparative study of antibacterial assays of Carumroxburghianum and Trigonellafoenum graecum obtained
... phytochemical screening of ajwan can be compared with phytochemical assay of radhuni seed extract in this ...Phytochemical screening was done for alkaloids, terpenoids, tannins, saponins, steroids, phenolic ... See full document
7
Paxillin dependent regulation of apical basal polarity in mammary gland morphogenesis
... epithelial cell polarity is fundamental for mammary gland duct morphogenesis during mammalian ...mesenchymal cell adhesion signaling, F-actin cytoskeleton remodeling and single cell migration, its ... See full document
15
Vol. 2, Issue 9, September 2013
... Virtual Screening : Molecular recognition [7] is the fundamental basis for drug action in which drug molecules exhibit pharmacological activity by binding to a target protein and forming a stable protein–ligand ... See full document
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