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A plasmid-based reverse genetics system for influenza A virus.
... the plasmid-based reverse genetics system for influenza virus described in this report allows intracellular re- constitution of viral RNPs and permits us to study the ... See full document
5
A One-Plasmid System To Generate Influenza Virus in Cultured Chicken Cells for Potential Use in Influenza Vaccine
... used influenza vaccines for human use are the inactivated and attenuated forms of the virus and are admin- istered via either the intramuscular or the intranasal ...one plasmid encoding proteins and ... See full document
8
The genetics of virus particle shape in equine influenza A virus
... equine influenza strain, Miami/63, was similarly diffusely distributed with no obvious filaments ...released virus particles, cell-free material from Miami/63 and Nkt/11/03 was examined by negative stain ... See full document
9
Characterization of Influenza Virus NS1 Protein by Using a Novel Helper-Virus-Free Reverse Genetic System
... A virus contains eight species of negative-sense viral RNAs as the ...infectious influenza A viruses had been developed (6, 7, 18), in which RNPs were reconstituted in vitro by transcribing RNAs from cloned ... See full document
6
Plasmid-Driven Formation of Influenza Virus-Like Particles
... The influenza virus life cycle begins with binding of the hemagglutinin (HA) to sialic acid-containing receptors on the surface of the host cell (reviewed in reference 6), followed by receptor-mediated ... See full document
5
Establishment of Canine RNA Polymerase I-Driven Reverse Genetics for Influenza A Virus: Its Application for H5N1 Vaccine Production
... H5N1 influenza A viruses continue to cause fatal human ...where influenza A is epidemic have expanded from Asia to Europe and Africa, raising concerns over a possible pandemic ...seed virus generated ... See full document
5
Identification of Novel Fusion Inhibitors of Influenza A Virus by Chemical Genetics
... of influenza A virus that act by interfering with the structural change of HA at acidic pH, a process necessary for successful entry of the ...for influenza A viruses and further shows that analysis ... See full document
12
Efficient generation of influenza virus with a mouse RNA polymerase I driven all in one plasmid
... combat influenza virus infections, the inactivated and attenuated vaccines remain the most effective tools ...an influenza pandemic or huge flocks of ...novel influenza vaccine which composes ... See full document
10
Cross-Reactive Immunity to Clade 2 Strains of Influenza Virus A Subtype H5N1 Induced in Adults and Elderly Patients by Fluval, a Prototype Pandemic Influenza Virus Vaccine Derived by Reverse Genetics, Formulated with a Phosphate Adjuvant, and Directed to Clade 1 Strains
... wild-type virus by classical reassortant technology in the biosafety level 3 facility of Omninvest ...non-H5N1 influenza virus strain A/Solomon Island/13/2006(H1N1)-like IVR-145 reassortant (kindly ... See full document
7
Inhibition of Human Immunodeficiency Virus Type 1 Replication prior to Reverse Transcription by Influenza Virus Stimulation
... HIV-1 reverse transcription and viral transcription have shown inhibition of HIV replication postintegration, at the level of transcription (13, ...to reverse transcription because the levels of both late ... See full document
7
Influenza Virus Hemagglutinin and Neuraminidase, but Not the Matrix Protein, Are Required for Assembly and Budding of Plasmid-Derived Virus-Like Particles
... in influenza virus ...in virus replication of a proline residue conserved among influ- enza A and B viruses, suggesting the importance of the sec- ondary structure of the NA cytoplasmic tail (5, ... See full document
13
Improvement of Influenza A/Fujian/411/02 (H3N2) Virus Growth in Embryonated Chicken Eggs by Balancing the Hemagglutinin and Neuraminidase Activities, Using Reverse Genetics
... H3N2 influenza A/Fujian/411/02-like virus strains that circulated during the 2003-2004 influenza season caused influenza ...A/Fujian/411/02 virus lineages did not replicate well in ... See full document
9
Universal Influenza B Virus Genomic Amplification Facilitates Sequencing, Diagnostics, and Reverse Genetics
... seasonal influenza virus vaccine com- ponents typically contain the hemagglutinin (HA) and neuramin- idase (NA) antigens of an H1N1 virus, an H3N2 virus, and a B/Victoria or B/Yamagata lineage ... See full document
8
Human RNA Polymerase I-Driven Reverse Genetics for Influenza A Virus in Canine Cells
... RG system (7) could rescue influenza viruses in the three cell ...optimized virus rescue pro- ...the influenza genome segments (1 g each), together with the TMPRSS2 helper plasmid, ... See full document
5
Interdependence of Hemagglutinin Glycosylation and Neuraminidase as Regulators of Influenza Virus Growth: a Study by Reverse Genetics
... pPolI-RT plasmid described earlier (30) and contains a truncated version of the human PolI promoter and a hepatitis virus delta ribozyme separated by SapI ...the influenza virus polymerase ... See full document
8
All-in-One Bacmids: an Efficient Reverse Genetics Strategy for Influenza A Virus Vaccines
... against influenza virus infection, and it is considered the first line of defense against ...Human influenza vaccines are produced as either split virion-inactivated (killed virus [KV]) ... See full document
13
Reverse genetics provides direct evidence for a correlation of hemagglutinin cleavability and virulence of an avian influenza A virus.
... In CEF, the plaqueforming ability of each virus clearly correlated with the HA cleavability determined by RIP assay Table 1; viruses with HAs that were not cleaved by the endogenous prot[r] ... See full document
9
Reverse Genetics System for Uukuniemi Virus (Bunyaviridae): RNA Polymerase I-Catalyzed Expression of Chimeric Viral RNAs
... pRF33. Based on the results from the influenza system, pol I-driven transcription of the reporter RNAs will initiate and terminate exactly at the 5⬘ and 3⬘ of the inserted cDNAs, thus giving rise to ... See full document
13
PCR based reverse genetics strategy for bluetongue virus recovery
... RG system, making the system much more versatile and generally applicable ...RG system al- though VP7 plasmid can be omitted ...each plasmid was adjusted to 100 ng/ μL with ...recover ... See full document
9
Cloning of the canine RNA polymerase I promoter and establishment of reverse genetics for influenza A and B in MDCK cells
... (characteristic virus induced cytopathic ...and virus titrated by plaque assay on MDCK cells followed by immunostaining, as described above, to confirm the influenza virus ... See full document
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