[PDF] Top 20 A point mutational analysis of human papillomavirus type 16 E7 protein.
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A point mutational analysis of human papillomavirus type 16 E7 protein.
... It is evident from these studies that mutants which destroy both transforming and trans-activation activities p24GLY and mutants which are transformation competent but express no or very[r] ... See full document
7
Purification and characterization of human papillomavirus type 16 E7 protein with preferential binding capacity to the underphosphorylated form of retinoblastoma gene product.
... 4966-4972 0022-538X/91/094966-07$02.00/0 Copyright © 1991, American Society for Microbiology Purification and Characterization of Human Papillomavirus Type 16 E7 Protein with Preferentia[r] ... See full document
7
Analysis of the p53-mediated G1 growth arrest pathway in cells expressing the human papillomavirus type 16 E7 oncoprotein.
... the E7-expressing clones, consistent with the FACS analysis data, verifying that the cells were continuing to ...decreased protein sta- bility. Northern blot analysis was performed to ... See full document
8
Leaky Scanning Is the Predominant Mechanism for Translation of Human Papillomavirus Type 16 E7 Oncoprotein from E6/E7 Bicistronic mRNA
... HPV type 16 (HPV-16) and related types are classed as high-risk viruses because of their association with high-grade CIN and cervical carcinoma (reviewed in reference ...Transcriptional ... See full document
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Mutational analysis of cis elements involved in E2 modulation of human papillomavirus type 16 P97 and type 18 P105 promoters.
... Cells were transfected with pSV2CAT contains the SV40 enhancer and early promoter upstream of the CAT gene, no DNA none, a construct containing the wild-type HPV-18 LCR upstream of the C[r] ... See full document
11
Systematic Analysis of Residues in Conserved Region 3 of the Human Papillomavirus 16 E7 Oncoprotein
... as human papillomaviruse have all been shown to encode viral gene products which manipulate the Rho-family GTPase signaling pathway (9, 17, 21, 35, ...HPV38 E7 protein affects actin stress fiber ... See full document
190
Squamous epithelial hyperplasia and carcinoma in mice transgenic for the human papillomavirus type 16 E7 oncogene.
... developed skin tumors late in life (Table 1). Grossly, these tumors belong to two classes, relatively small (,1 cm) but highly keratotic lesions and large, cystic but nonkeratotic le- sions. These tumors arose on the ... See full document
14
Anchorage-Independent Transcription of the Cyclin A Gene Induced by the E7 Oncoprotein of Human Papillomavirus Type 16
... of E7-expressing but not control ...question, E7-express- ing and control cells were subjected to mild lysis and then nuclei were separated from the cytoplasmic ...use E7/2 cells rather than ... See full document
12
The E7 Oncoprotein of Human Papillomavirus Type 16 Stabilizes p53 through a Mechanism Independent of p19ARF
... in E7-expressing cells was due to a decrease in the ability of MDM2 and p53 to ...from E7-expressing ...p53 protein in 150 g of each total lysate to the amount in the immunoprecipitates from 150-g ... See full document
9
The Human Papillomavirus Type 16 E7 Oncogene Is Required for the Productive Stage of the Viral Life Cycle
... oncogene E7 is not necessary for HPV-16 DNA replication during the nonproductive stage of the viral life ...of E7 in the HPV-16 life ...the E7-deficient HPV-16 genome episo- ... See full document
10
Mutational analysis of human papillomavirus type 16 E7 functions.
... Whereas the immortalizing function transformation in conjunction with activated ras for primary rat cells paralleled the transforming function for rat 3Y1 cells, the transformation funct[r] ... See full document
8
Human Papillomavirus Type 16 E6/E7 Upregulation of Nucleophosmin Is Important for Proliferation and Inhibition of Differentiation
... a protein showing in- creased levels compared to the vector control ...by E7 at the protein level through the ability of E7 to deregulate v-akt murine thymoma viral oncogene (AKT) and that ... See full document
9
E6 and E7 from Human Papillomavirus Type 16 Cooperate To Target the PDZ Protein Na/H Exchange Regulatory Factor 1
... and E7 cooperate in promoting NHERF-1 deg- ...primary human foreskin keratino- cytes ...HPV16 E7/E6-expressing retrovirus, and NHERF-1 levels were determined by immunoblotting and by immunofluores- ... See full document
9
Human Papillomavirus Type 16 E5 Protein as a Therapeutic Target
... E6 protein is a 151-amino acid pro- tein, containing two zinc finger ...primary human foreskin keratinocytes (HFK), but can efficiently immor- talize human mammary epithelial cells ...the E7 ... See full document
14
Mutational analysis of human papillomavirus type 16 E6 demonstrates that p53 degradation is necessary for immortalization of mammary epithelial cells.
... HPV16 E6 constructs and mutagenesis. HPV16 E6 was PCR amplified as two fragments, using the oligonucleotide primers 59 gcg gtc gac ATG CAC CAA AAG AGA AC 39, 59 TCT TCC GGA CAC AGT GGC 39, 59 GTG TCC GGA AGA AAA GC 3 9 , ... See full document
6
Abrogation of growth arrest signals by human papillomavirus type 16 E7 is mediated by sequences required for transformation.
... mutated E7 proteins that were able to disrupt suprabasal quiescence similar to the wild-type 16E7 protein include D21S, D36H, A50S, and S71I ...mutated E7 proteins that failed to disrupt ... See full document
8
Identification of the FHL2 Transcriptional Coactivator as a New Functional Target of the E7 Oncoprotein of Human Papillomavirus Type 16
... activator protein-1 (AP- 1)-driven transcription ...of human tumors (1). To address the influence of HPV-16 E7 on the ability of FHL2 to coactivate AP-1-driven transcription, the AP-1 ... See full document
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E6^E7, a Novel Splice Isoform Protein of Human Papillomavirus 16, Stabilizes Viral E6 and E7 Oncoproteins via HSP90 and GRP78
... bovine papillomavirus 1 (BPV-1)-transformed mouse C127 cells showed that the BPV-1 E6 ORF could be spliced to the E7 ORF in frame, leading to the production of a novel E6^E7 mRNA to encode a ... See full document
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Chemical synthesis of human papillomavirus type 16 E7 oncoprotein: autonomous protein domains for induction of cellular DNA synthesis and for trans activation.
... DNA synthesis induction activity maps within the N-terminal portion of the molecule, which contains sequences related to adenovirus ElA conserved domains 1 and 2 required for cell transf[r] ... See full document
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Accumulation of human papillomavirus type 16 E7 protein bypasses G1 arrest induced by serum deprivation and by the cell cycle inhibitor p21.
... To determine the effects of E7 expression on the activities of cdks, the extracts from infected cells were compared for cdk activities. These extracts contained comparable levels of the cdc2, cdk2, and cdk4 ... See full document
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