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[PDF] Top 20 Pyruvate production using engineered Escherichia coli

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Pyruvate production using engineered Escherichia coli

Pyruvate production using engineered Escherichia coli

... E. coli cells, the accABCD genes are annotated as essential genes for ...of pyruvate (Nakashima et ...greater pyruvate accumulation (Nakashima et ...that pyruvate accumulation is achieved by ... See full document

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Vanillin production using metabolically engineered Escherichia coli under non-growing conditions

Vanillin production using metabolically engineered Escherichia coli under non-growing conditions

... The response surface methodology (RSM) proved to be an effective way to further optimize the process. The highest vanillin molar yield (about 75%) was achieved at low fer- ulic acid concentrations (0.5 mM) using ... See full document

11

Electrode-assisted acetoin production in a metabolically engineered Escherichia coli strain

Electrode-assisted acetoin production in a metabolically engineered Escherichia coli strain

... acetoin production in E. coli were around 80% of the theoretical maximum (Table ...cal production, due to the necessity of aeration and the higher ratio of anabolism over catabolism ...E. coli ... See full document

11

Efficient synthesis of L-lactic acid from glycerol by metabolically engineered Escherichia coli

Efficient synthesis of L-lactic acid from glycerol by metabolically engineered Escherichia coli

... L-lactate production from sugars can be achieved using native lactic acid bacteria but are constrained by the requirements for complex nutrients and exhibit limitations in both product selectivity and ... See full document

11

Renewable synthesis of n-butyraldehyde from glucose by engineered Escherichia coli

Renewable synthesis of n-butyraldehyde from glucose by engineered Escherichia coli

... n-Butyraldehyde is an intermediate in the Clostridium CoA-dependent n-butanol production pathway [9, 13, 14] (Fig. 1b). However, bifunctional aldehyde/alcohol dehydrogenase AdhE2 catalyzes the direct two-step ... See full document

10

Retinoid production using metabolically engineered Escherichia coli with a two-phase culture system

Retinoid production using metabolically engineered Escherichia coli with a two-phase culture system

... Retinoid production was maxi- mal at these times and then significantly decreased dur- ing stationary ...Retinoid production appeared to increase mainly after 24 ...noid production (95 mg/L) was ... See full document

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MEP pathway-mediated isopentenol production in metabolically engineered Escherichia coli

MEP pathway-mediated isopentenol production in metabolically engineered Escherichia coli

... isopentenol production of engineered E. coli by opti- mizing its inherent MEP pathway (Figure ...E. coli W3110 to confer its production of isopentenols including both prenol and ...E. ... See full document

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Production of free monounsaturated fatty acids by metabolically engineered Escherichia coli

Production of free monounsaturated fatty acids by metabolically engineered Escherichia coli

... E. coli BL21 ...E. coli competent cells that car- ried the Red recombinase expression vector pKD46 and integrated into its ...performed using primer pairs de- signed according to the sequences up- ... See full document

11

Dehydratase mediated 1-propanol production in metabolically engineered Escherichia coli

Dehydratase mediated 1-propanol production in metabolically engineered Escherichia coli

... E. coli gldA in a strain defecient in lactate production and using an initial glucose concentration of ...Enhanced production of 1,2-propanediol in E. coli was also reported by Altaras ... See full document

10

Modular design of metabolic network for robust production of n-butanol from galactose–glucose mixtures

Modular design of metabolic network for robust production of n-butanol from galactose–glucose mixtures

... we engineered robust Escherichia coli to accomplish high production of n-butanol from galactose–glucose mixtures via the design of modular pathway, an efficient and systematic way, to ... See full document

8

Eliminating acetate formation improves citramalate production by metabolically engineered Escherichia coli

Eliminating acetate formation improves citramalate production by metabolically engineered Escherichia coli

... acetate production pathways in ...E. coli strain ...study using defined medium suggests that acetate is indeed formed from glucose rather than from an unknown com- ponent in a complex ... See full document

10

Efficient bio production of citramalate using an engineered
Escherichia coli strain

Efficient bio production of citramalate using an engineered Escherichia coli strain

... process using glucose alone (based on current prices; Table ...with using glucose only (Table ...patented production strain and fermentation process, since we obtained citramalate production ... See full document

9

Deoxycytidine production by a metabolically engineered Escherichia coli strain

Deoxycytidine production by a metabolically engineered Escherichia coli strain

... Interestingly, HPLC analysis of the culture superna- tants of HLC010-pETNGY and HLC010-pETGY broth revealed the formation of byproducts in addition to dCyd production (Additional file 1: Figure S5), which poten- ... See full document

11

An Engineered Synthetic Pathway for Discovering Nonnatural Nonribosomal Peptides in Escherichia coli

An Engineered Synthetic Pathway for Discovering Nonnatural Nonribosomal Peptides in Escherichia coli

... cells using specialized transporters (14), and antibiotics that have evolved to structurally resemble siderophores (58) can take advantage of this mechanism to kill bacterial ... See full document

16

Fermentative production and direct extraction of (−)-α-bisabolol in metabolically engineered Escherichia coli

Fermentative production and direct extraction of (−)-α-bisabolol in metabolically engineered Escherichia coli

... improved production of ...E. coli cell growth ...(−)-α-bisabolol production, overexpression of an FPP synthase, encoded by the ispA gene, was ...E. coli was inserted downstream of the MrBBS ... See full document

13

Production of p-amino-l-phenylalanine (l-PAPA) from glycerol by metabolic grafting of Escherichia coli

Production of p-amino-l-phenylalanine (l-PAPA) from glycerol by metabolic grafting of Escherichia coli

... E. coli. These recipients often have been engineered for a fortified pro- vision of chorismate from the general aromatic amino acid pathway [61, 66, 69, ...of using North American vine as rootstock ... See full document

11

Production of extracellular fatty acid using engineered Escherichia coli

Production of extracellular fatty acid using engineered Escherichia coli

... These medium and long chain fatty acids could cause severe foaming problem during the cultivation process. The more fatty acids were produced, the more serious the foaming problem was. If the foam could not be con- ... See full document

13

Synthesis of avenanthramides using engineered Escherichia coli

Synthesis of avenanthramides using engineered Escherichia coli

... Next, we synthesized avn F. One additional gene, HpaBC, which was reported to convert p-coumaric acid into caffeic acid [31] (Additional file 1), was introduced into HA-7 (strain HA-Hpa in Table 3). The resulting strain ... See full document

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Complex Multilevel Control of Hemolysin Production by Uropathogenic Escherichia coli

Complex Multilevel Control of Hemolysin Production by Uropathogenic Escherichia coli

... Hemolysin gene transcription and hemolysin expression correlate with the level of hemolytic activity. To explore the basis of differential hemolytic activity in ST131, we compared the hlyA and hlyC transcript levels from ... See full document

17

Synthetic pathways and processes for effective production of 5-hydroxytryptophan and serotonin from glucose in Escherichia coli

Synthetic pathways and processes for effective production of 5-hydroxytryptophan and serotonin from glucose in Escherichia coli

... serotonin production from a simple carbon source, final production achieved was ...mg/L using the stepwise culture ...tryptamine production, reducing the formation of byproducts during the ... See full document

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