[PDF] Top 20 Quantitative Analysis of Gamma Interferon Release Assay Response in Children with Latent and Active Tuberculosis

... of interferon gamma (IFN- ␥ ) release assays (IGRAs) for the diag- nosis of tuberculosis (TB) infection in children is still under debate because of con- cerns about the immature immune ... See full document

... treated with anti-TB prophylactic agents [7]. However evidence suggests that the currently available treatments for LTBI are effective in preventing a reactivation of TB (60–90%) [5], hence treatments would have had an ... See full document

... of active disease, each of the 29 individuals considered positive for LTBI, by either one or both IGRAs at either day 0 or day 7, underwent and completed treatment without ...in response to ESAT-6 and ... See full document

... GIT assay have shown that while there is a high degree of agree- ment between dichotomous results, there is considerable variabil- ity in quantitative results, which leads to discordant results when the TB ... See full document

... and latent TB infections, especially in settings of high HIV ...The gamma interferon (IFN- ␥ ) release assay (IGRA) is an in vitro test based on release of IFN- ␥ by foreign ... See full document

... In our study, T-SPOT.TB outperformed QFG. The proportion of individuals testing positive has also been found significantly greater with T-SPOT.TB than with QFG in other studies conducted in asymptomatic HIV- infected ... See full document

... immunocompromised children was evaluated as a screening for LTBI with no case of active TB disease included [53-57], and concordance between tests was ...immune-compromised children both in high- and ... See full document

... until analysis. Concentrations of interferon-gamma (INF-g ) in 50 mL of plasma from whole-blood stimulated with the antigens, mitogen (positive control) or saline (negative control) were measured ... See full document

... for latent TB using the HBHA-IGRA in parallel to a classical method consisting of medical history, chest X-ray, tuberculin skin test (TST) and QuantiFERON®-TB Gold In-Tube ...of active TB was also recruited ... See full document

... T-cell-based gamma interferon (IFN- ␥ ) release assays (IGRAs) using Mycobacterium tuberculosis-specific antigens have shown higher sensitivity and specificity than the routine tuberculin skin ... See full document

... mycobacterium tuberculosis (MTb), and the host’s innate and adaptive immune ...immune response shortly after infection results in asymptomatic infection, which may remain undiagnosed and could reactivate ... See full document

... Univariate analysis was performed and HIV negative status was found to be a risk factor for a positive test for TST (OR = ...univariate analysis. BCG vaccination, household con- tact with a case of ... See full document

... Bayesian latent class analysis of published data on the performance of TST and IGRAs for the diagnosis of ...with active TB as a surrogate for ...immune response, which is the measure of QFT, ... See full document

... the quantitative re- sponse of IGRA is higher in active TB than in latent TB, and generally falls during anti-TB medication [13, ...high response of IGRA is associ- ated with disease burden ... See full document

... QFT assay. The QFT assay was performed in accordance with the manufac- turer’s instructions included in the package ...M. tuberculosis infection was indicated by a human response (Table 1, ... See full document

... with active TB and healthy sensitized donors and that CyaA delivery decreased the molar amount of antigen required to restimulate T cells ...in response to test ... See full document

... Since vaccination data was either not available or in- complete for some participants, all TST-positive cases were then tested also with the QuantiFERON® TB-Gold (QFT) kit (Cellestis, Carnegie, Australia), a second- ... See full document

... with active TB were eligible for this study; eight were lost to ...M. tuberculosis cultured from their ...of active TB during the follow- up period of 102 ± 105 days (range, ... See full document

... In the current study, three strains of M. bovis were used to compare IFN-␥ responses to PPDs and peptide cocktails elicited after experimental infection. With the use of the IFN- ␥ release assay, robust ... See full document

... As shown in Table 2, mean square error indicating magni- tude of within-individual error was large in all conditions of the first trial, which was indicated by remarkably large CV (> 20%) for Nil, TB-Antigen and ... See full document