[PDF] Top 20 Rapid Detection of Shigella flexneri on Egg Samples by the Real Time Polymerase Chain Reaction Method
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Rapid Detection of Shigella flexneri on Egg Samples by the Real Time Polymerase Chain Reaction Method
... of Shigella species with the Real Time PCR method in food samples and naturally contaminated stool samples ...food samples used within this study were egg ...of ... See full document
7
Real-Time Polymerase Chain Reaction for the Rapid Detection of Group B Streptococcal Colonization in Neonates
... ever, detection of surface colonization using a sensitive screening method would identify infants who are poten- tially at risk for invasive GBS ...enhanced detection of GBS colonization by ... See full document
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Quantitation of Pseudomonas aeruginosa in wound biopsy samples: from bacterial culture to rapid ‘real time’ polymerase chain reaction
... lower detection limit of 10 3 CFU/g tissue, ELISA-PCR was ten times more sensitive than conventional ...very time consuming (8 ...quantitative detection range of 7 logs with a lower detection ... See full document
7
Evaluation of pathogen detection from clinical samples by real time polymerase chain reaction using a sepsis pathogen DNA detection kit
... DNA Detection Kit analysis could not detect all sepsis pathogens, SeptiFast analysis did detect poten- tially important pathogenic DNA that could not be detected by blood culture ...of samples from patients ... See full document
9
Effectiveness of real-time polymerase chain reaction assay for the detection of Mycobacterium tuberculosis in pathological samples: a systematic review and meta-analysis
... condition. Polymerase chain reac- tion (PCR) technology was introduced in the mid-1990s and has revolutionised the diagnosis of infectious dis- ...for rapid and specific detection of MTB com- ... See full document
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RAPID DETECTION OF Salmonella IN SHRIMP BY POLYMERASE CHAIN REACTION [Deteksi Cepat Salmonella pada Udang dengan Polymerase Chain Reaction]
... Salmonellae rapid detection method in shrimp by ...protocol method to detect Salmonella invA gene was conducted using six different annealing temperatures (59, ...based detection ... See full document
5
Development of real time polymerase chain reaction for detection of Salmonella typhimurium and Salmonella enteritidis in fish
... Additional samples were spiked below the direct detection limit in which resulting statistical differences were being identified, but based on the current statistical result analysis of Salmonella in fish ... See full document
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Original Article Quantitative real-time polymerase chain reaction is an alternative method for the detection of HER-2 amplification in formalin-fixed paraffin-embedded breast cancer samples
... In our study, the qPCR results were in perfect concordance with those of IHC and FISH. Chromosome multi-body and morphological heterogeneity have been some of the difficul- ties in the diagnosis of breast cancer, such as ... See full document
10
Detection of foot-and-mouth disease virus in milk samples by real-time reverse transcription polymerase chain reaction: Optimisation and evaluation of a high-throughput screening method with potential for disease surveillance
... serum samples, and opportunistic milk samples collected in the field during the UK 2007 outbreak ( Ryan et ...milk samples collected from the UK 2007 outbreak generated lower C T values for all ... See full document
6
Original Article Detection of toxin genes from Clostridium difficile by using real-time polymerase chain reaction
... Statistical analysis For the statistical comparison of routine toxi- genic culture-negative samples, a regression method for paired samples was used [18]. The outputs were relative positive fractions ... See full document
7
Development of Real-Time Polymerase Chain Reaction Assays for the Detection and Differentiation of Infectious Bursal Disease Virus Subtypes
... IBDV samples revealed that the residues involved in determining the virulence of VV IBDV and CL IBDV were highly ...duplex real-time PCR assay development, a new set of primers FWDC and RVSC were ... See full document
25
Detection of Shigella dysenteriae type 1 and Shigella flexneri in feces by immunomagnetic isolation and polymerase chain reaction
... ern blot hybridization (II) of the 320-bp PCR products with the AP probe. The amplified samples are cell lysate of IMS-isolated S. dysentenae type 1 culture positive, lane 6), whole-cell[r] ... See full document
6
Polymerase chain reaction for detection of adenoviruses in stool samples
... Minute amounts of adenovirus particles are frequently shed since, after amplification and through replication in cell cultures, 51 of 60 samples (corresponding to 85%) were positive for [r] ... See full document
10
Polymerase chain reaction for detection of Mycoplasma genitalium in clinical samples
... On the other hand, all the amplified fragments hybridized strongly to the MgPa-2 probe and possessed DdeI, AluI, and Sau3AI restriction enzyme cleav- age sites, precluding the possibilit[r] ... See full document
5
Polymerase chain reaction for detection of Leptospira spp in clinical samples
... Southern blot hybridization with a digoxygenin-labeled probe of PCR products (30 cycles with primers A and B) of urine specimens seeded with serially diluted L. interrogans serovar ict- [r] ... See full document
6
RAPID POLYMERASE CHAIN REACTION METHOD FOR DETECTION OF VIBRIO CHOLERAE IN FOOD AND THEIR CYTOTOXIC EFFECT
... optimized for determining the presence enterotoxigenic Vibrio cholera in food. In the present study, ToxR primers were used for the identification of Vibrio cholera strains DNA were amplified and showed a band under the ... See full document
5
Rapid detection of bovine viral diarrhea virus by polymerase chain reaction
... (a) Agarose gel electrophoresis of PCR products, with cell-propagated reference strains and spiked BVDV strain NADL serum used as targets for amplification. Arrowhead indicates the 205-b[r] ... See full document
5
RAPID DETECTION OF MYCOBACTERIUM TUBERCULOSIS IN CLINICAL SPECIMENS BY POLYMERASE CHAIN REACTION
... Taq polymerase (Perkin-Elmer Cetus, Norwalk, ...the detection of amplifiable DNA purified from each ...all reaction steps gloves were worn and changed ... See full document
6
Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase polymerase chain reaction
... As previously stated, the RNA isolated from these infected mosquitoes was originally found negative for dengue type 2 virus by the RT-PCR assay at all time points. However, when the RNA [r] ... See full document
7
A simple and rapid method for detection of Trypanosoma evansi in the dromedary camel using a nested polymerase chain reaction
... Extraction of DNA from blood samples Whole blood was used for extraction of total genomic DNA using commercially available QIAamp blood kit (QIAGEN Inc. Chatsworth, CA, USA) according to the manufacturer's ... See full document
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