[PDF] Top 20 Rapid detection of wheat yellow mosaic virus by reverse transcription loop mediated isothermal amplification
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Rapid detection of wheat yellow mosaic virus by reverse transcription loop mediated isothermal amplification
... MLV reverse transcriptase instead of AMV reverse tran- scriptase under isothermal condition at 65°C for 80 ...further virus detection in this ...and virus was detected after ... See full document
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Rapid Detection and Differentiation of Dengue Virus Serotypes by a Real Time Reverse Transcription Loop Mediated Isothermal Amplification Assay
... dengue virus serotype-specific reverse transcription–loop-mediated isothermal amplification (RT-LAMP) assay targeting the 3 ⴕ noncoding region for the rapid ... See full document
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Development and Evaluation of a Reverse Transcription Loop Mediated Isothermal Amplification Assay for Rapid Detection of Enterovirus 71
... and rapid detection is critical for prevention and control of EV71 infection, since no vaccine or antiviral drugs are currently ...sensitive reverse transcription-loop-mediated ... See full document
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Real Time Reverse Transcription Loop Mediated Isothermal Amplification for Rapid Detection of Rift Valley Fever Virus
... The RT-LAMP assay requires six primers that recognize eight distinct regions of the target and, when properly de- signed, prevent cross-reactions (Table 2). Due to the setting of these primers in a highly conserved RVFV ... See full document
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Rapid detection of Akabane virus by a novel reverse transcription loop mediated isothermal amplification assay (RT LAMP)
... first amplification after reverse transcription reaction, while N1 and N3 were used for the second round of amplification (semi- ...III Reverse Transcriptase kit (Invitrogen) following ... See full document
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Rapid Detection of Norovirus from Fecal Specimens by Real Time Reverse Transcription Loop Mediated Isothermal Amplification Assay
... RNA amplification kit (Eiken Chemical, Tochigi, ...of loop primers LF1, LF2, and LF3 for GI or 20 pmol each of loop primers LF1, LF2, LF3, and LF4 for GII, and 2 l of RNA ...the detection ... See full document
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Rapid detection of newly isolated Tembusu related Flavivirus by reverse transcription loop mediated isothermal amplification assay
... Based on the sequence of newly isolated Tembusu- related Flavivirus BYD-1 published in GenBank (Gen- bank accession no.JF312912.1) and other unpublished sequences that we collected, a highly conserved region of the E ... See full document
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Development and evaluation of reverse transcription loop mediated isothermal amplification assay for rapid detection of enterovirus 71
... Six primers were designed according to the VP2 gene sequences of EV71 virus published in GenBank (acces- sion No. AY465356), and was aligned with available VP2 gene sequences of other strains, including the ... See full document
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Rapid detection of porcine kobuvirus in feces by reverse transcription loop mediated isothermal amplification
... this virus is still limited and it is uncertain whether porcine kobuvirus is involved in pig diarrhea as it can be detected in both diar- rhea and healthy ... See full document
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Reverse transcription, loop mediated isothermal amplification assay for the sensitive and rapid detection of H10 subtype avian influenza viruses
... The HA gene sequences of the H10 subtype AIVs were available downloaded from the NCBI Influenza Virus Resource Database and were compared and an- alyzed using the DNAstar software to identify con- served regions. ... See full document
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Real Time Reverse Transcription Loop Mediated Isothermal Amplification for Rapid Detection of West Nile Virus
... (WN) virus, an arthropod-borne virus, is a mem- ber of the Japanese encephalitis (JE) virus serocomplex of the family Flaviviridae, genus ...The virus has a positive- sense single-stranded RNA ... See full document
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Development of a real time reverse transcription loop mediated isothermal amplification method for the rapid detection of porcine epidemic diarrhea virus
... The isothermal conditions re- quired for LAMP can be provided with a conventional water bath or heat block, which can be applied in less well-equipped laboratories and fields for rapid detection of ... See full document
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Evaluation of Reverse Transcription Loop Mediated Isothermal Amplification assays for Rapid Detection of Human Enterovirus 71 and Coxsackievirus A16 in Clinical Samples
... nested reverse transcription polymerase chain reaction (nRT-PCR) assay and sequencing to be true positives for ...the rapid and visual screening of EV71 and CVA16 in China, especially in those ... See full document
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Development and Evaluation of Reverse Transcription Loop Mediated Isothermal Amplification Assay for Rapid and Real Time Detection of Japanese Encephalitis Virus
... Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification method developed by Eiken Chem- ical ...The amplification efficiency of the LAMP method is ... See full document
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Rapid and Sensitive Detection of H7N9 Avian Influenza Virus by Use of Reverse Transcription–Loop Mediated Isothermal Amplification
... influenza virus infection recently emerged in China whose clinical features include high mortality and which has also resulted in serious economic ...(H7N9) virus which was the causative agent of this ... See full document
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Rapid and Real Time Detection of Chikungunya Virus by Reverse Transcription Loop Mediated Isothermal Amplification Assay
... Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification method developed by Eiken Chem- ical ...gene amplification tool for the rapid ... See full document
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Detection of New Bunyavirus RNA by Reverse Transcription–Loop Mediated Isothermal Amplification
... for rapid detection of other bunyaviruses, includ- ing Rift Valley fever virus (RVFV) and Crimean Congo hemor- rhagic fever virus (CCHFV) (16, 17); thus, it can be anticipated that RT-LAMP for ... See full document
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Detection of nucleic acid of classical swine fever virus by reverse transcription loop mediated isothermal amplification (RT LAMP)
... Reverse transcription loop-mediated isothermal am- plification (RT-LAMP) was used as tool for amplifica- tion and has emerged as a powerful gene amplification tool due to its ... See full document
6
Visual detection of H3 subtype avian influenza viruses by reverse transcription loop mediated isothermal amplification assay
... Loop-mediated isothermal amplification (LAMP) is a new nucleic acid amplification method that uses a set of six primers that target the eight site of a conserved gene, yielding a large ... See full document
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Detection of dengue viruses using reverse transcription loop mediated isothermal amplification
... of virus-specific antibodies as well as virus isolation. Detection of viral components such as non-structural protein 1 (NS1) and virus genomic RNA are done but mostly in referral or in more ... See full document
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