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Real Time Reverse Transcription Loop Mediated Isothermal Amplification for Rapid Detection of West Nile Virus

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Figure

TABLE 1. Details of oligonucleotide primers used for RT-LAMP amplification of E gene of WN virus
FIG. 1. (A) Agarose gel electrophoresis and restriction analysis of the RT-LAMP product of the Eladder (Sigma Genosys); 1, RT-LAMP with WN virus strain NY99; 2, RT-LAMP products digested withRNA
FIG. 2. (A) Effect of temperature on the time kinetics of the RT-LAMP reaction of WN virus strain NY99 as monitored by measurement ofturbidity in a Loopamp real-time turbidimeter (LA-200; Teramecs)
TABLE 2. Comparative sensitivities and specificities of the RT-LAMP and RT-PCR assay systems for detection of WN virusa

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