[PDF] Top 20 Rapid Multiplex Nested PCR for Detection of Respiratory Viruses
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Rapid Multiplex Nested PCR for Detection of Respiratory Viruses
... this multiplex nested PCR was its broader spectrum of ...the detection spec- trum has also been the main focus of previous studies, and as many as nine different respiratory pathogens ... See full document
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Comparison of the Eragen Multi Code Respiratory Virus Panel with Conventional Viral Testing and Real Time Multiplex PCR Assays for Detection of Respiratory Viruses
... High-throughput multiplex assays for respiratory viruses are an important step forward in diagnostic ...Multi-Code Respiratory Virus Panel (PLx-RVP), manufactured by Eragen Biosciences, ... See full document
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Development of a Respiratory Virus Panel Test for Detection of Twenty Human Respiratory Viruses by Use of Multiplex PCR and a Fluid Microbead Based Assay
... 20 respiratory viruses, including the conventional respiratory viruses influenza A and B viruses, parainfluenza virus types 1 to 4, RSV, adenovirus, MPV, com- mon cold viruses ... See full document
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Comparison between Saliva and Nasopharyngeal Swab Specimens for Detection of Respiratory Viruses by Multiplex Reverse Transcription PCR
... for multiplex real-time reverse transcription (RT)-PCR for respiratory virus ...all viruses tar- geted by multiplex ...acute respiratory illnesses. Specimens were tested for 16 ... See full document
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Evaluation of custom multiplex real time RT PCR in comparison to fast track diagnostics respiratory 21 pathogens kit for detection of multiple respiratory viruses
... respiratory viruses present with similar signs and symp- toms and can’t be differentiated symptomatically or clinic- ...of rapid simultaneous identification of various viruses at the same time ... See full document
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Detection of Respiratory Viruses in Sputum from Adults by Use of Automated Multiplex PCR
... with respiratory failure (10, 11). However, respiratory viruses have been detected in sputum samples from patients with chronic obstructive pulmonary dis- ease (COPD), asthma, and cystic fibrosis ... See full document
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Impact of Early Detection of Respiratory Viruses by Multiplex PCR Assay on Clinical Outcomes in Adult Patients
... a rapid anti- gen test was performed, data were also collected on reflex Prodesse testing whenever done (date and time of Prodesse result in ...negative rapid an- tigen test result was followed by positive ... See full document
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Accurate Detection of Avian Respiratory Viruses by Use of Multiplex PCR Based Luminex Suspension Microarray Assay
... Viral respiratory diseases are leading causes of economic losses in the poultry industry world- wide due to increased mortality, impaired growth, and reduced egg and meat ...virulent viruses, mainly ... See full document
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Multiplex reverse transcription PCR for surveillance of influenza A and B viruses in England and Wales in 1995 and 1996
... Multiplex PCR assays capable of amplifying 13 or more separate regions of chromosomal DNA and of detecting and typing several bacterial and viral pathogens have also been described (3; reviewed in reference ... See full document
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Stable and Noncompetitive RNA Internal Control for Routine Clinical Diagnostic Reverse Transcription PCR
... three multiplex nested RT-PCR assays for nine respiratory ...for detection was present in the ...of respiratory sample ... See full document
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Detection and Identification of Human Parainfluenza Viruses 1, 2, 3, and 4 in Clinical Samples of Pediatric Patients by Multiplex Reverse Transcription PCR
... Virus isolation. Human laryngeal epidermoid carcinoma (HEp-2) cells, human lung mucoepidermoid carcinoma (NCI-H292) cells, Madin-Darby canine kidney (MDCK) cells, and human embryonic lung fibroblast (Fp) cell cultures ... See full document
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Rapid and Sensitive Method Using Multiplex Real Time PCR for Diagnosis of Infections by Influenza A and Influenza B Viruses, Respiratory Syncytial Virus, and Parainfluenza Viruses 1, 2, 3, and 4
... real-time PCR was found to be more sensitive than cell culture on a range of different respiratory ...the detection of viral infections (3, 7, 9, 15, 22, 23, ...Conventional respiratory viral ... See full document
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Comparison of a Multiplex Reverse Transcription PCR Enzyme Hybridization Assay with Conventional Viral Culture and Immunofluorescence Techniques for the Detection of Seven Viral Respiratory Pathogens
... A multiplex reverse transcription-PCR-enzyme hybridization assay (RT-PCR-EHA; Hexaplex; Prodesse ...simultaneous detection of human parainfluenza virus types 1, 2, and 3, influenza virus types ... See full document
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A multiplex PCR assay for the detection of five influenza viruses using a dual priming oligonucleotide system
... based PCR is the blocking extension of non-specifically primed tem- plates under less than optimal PCR conditions ...different respiratory viruses ...based multiplex PCR with ... See full document
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A multiplex one tube nested real time RT PCR assay for simultaneous detection of respiratory syncytial virus, human rhinovirus and human metapneumovirus
... in detection of RSV, HRV and HMPV were more than 95% and the sensitivities of the mOTNRT-PCR assay were ...two-step nested PCR assay ... See full document
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Simultaneous detection of eight immunosuppressive chicken viruses using a GeXP analyser based multiplex PCR assay
... A total of 300 clinical specimens, including the thymus gland, spleen, bursa, bone marrow, blood and liver of diseased chickens, were collected from farms. These dis- eased chickens showed various symptoms, with most ... See full document
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Development and Application of a Multiplex PCR Method for Rapid Differential Detection of Subgroup A, B, and J Avian Leukosis Viruses
... kosis viruses was previously found in poultry ...subgroup viruses, such as ALV-J, with stronger pathogenicity and epidemicity (8, ...ing rapid clinical detection of infection is imperative for ... See full document
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A Nested Allele-Specific Multiplex Polymerase Chain Reaction Method for the Detection of DRD2 Polymorphisms
... the detection of DRD2 polymorphisms include polymerase chain reaction (PCR)–restriction fragment length polymorphism (RFLP) (5,6,8,16–19), denaturing gradient gel electrophoresis (6), Southern blot analysis ... See full document
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Development and evaluation of one step single tube multiplex RT PCR for rapid detection and typing of dengue viruses
... the viruses were propagated in C6/36 cells prior to extraction of ...4 viruses (Table ...and nested PCR. The RT-PCR and nested PCR revealed desired amplifica- tion as ... See full document
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Multiplex Real Time PCR Assay for Detection of Influenza and Human Respiratory Syncytial Viruses
... A multiplex real-time PCR assay was developed with a LightCycler instrument for detection of influenza viruses A and B and the human respiratory syncytial virus ...(HRSV). ... See full document
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