[PDF] Top 20 Real Time Sequencing of Mycobacterium tuberculosis: Are We There Yet?
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Real Time Sequencing of Mycobacterium tuberculosis: Are We There Yet?
... such time, if WGS is used to predict resistance, phenotypic DST should be performed in parallel—a prospect that is likely cost prohibitive for most TB control ... See full document
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Rapid Differentiation of Mycobacterium tuberculosis and M bovis by High Resolution Melt Curve Analysis
... a real-time PCR HRM assay on DNA extracted directly from positive Ziehl-Neelsen-stained sputum smear ...the real-time PCR HRM assay was found to be crucial for samples with a low bacterial ... See full document
5
Multiplex Real Time PCR Assay and Melting Curve Analysis for Identifying Mycobacterium tuberculosis Complex and Nontuberculous Mycobacteria
... over sequencing analysis was that it could directly identify mycobacteria from primary liquid detection media (in MGIT culture ...tion, sequencing analysis typically requires pure isolates, which are grown ... See full document
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Comparison of the Xpert MTB/RIF Test with an IS6110 TaqMan Real Time PCR Assay for Direct Detection of Mycobacterium tuberculosis in Respiratory and Nonrespiratory Specimens
... of Mycobacterium tuberculosis complex (MTBC) in clinical ...of real-time PCR testing platforms. The main advantages of real-time PCR are a shortened turnaround time; ... See full document
5
Clinical use of whole genome sequencing for Mycobacterium tuberculosis
... The reliability of the genotype–phenotype prediction is dependent not only on good correlation data but also on good database resources to allow easy access to such data [42]. TBDReaMDB [43] was the first TB-specific ... See full document
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Rapid and Specific Detection of Mycobacterium tuberculosis from Acid Fast Bacillus Smear Positive Respiratory Specimens and BacT/ALERT MP Culture Bottles by Using Fluorogenic Probes and Real Time PCR
... A real-time PCR assay using the LightCycler (LC) instrument for the specific identification of Mycobacterium tuberculosis complex (MTB) was employed to detect organisms in 135 acid-fast ... See full document
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Rapid Identification of Mycobacterium tuberculosis and Nontuberculous Mycobacteria by Multiplex, Real Time PCR
... measures, yet there are few commercially available assays that can easily and accurately identify the mycobacteria in culture in a timely ...Here we report on the development of a multiplex, ... See full document
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Detection of Mycobacteria, Mycobacterium avium Subspecies, and Mycobacterium tuberculosis Complex by a Novel Tetraplex Real Time PCR Assay
... genus Mycobacterium encompasses a large number of worldwide distributed pathogenic and opportunistic bacteria responsible for a broad range of infectious ...rium tuberculosis complex, Mycobacterium ... See full document
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Rapid Determination of Rifampin Resistance in Clinical Isolates of Mycobacterium tuberculosis by Real Time PCR
... by real-time PCR were compared to those obtained by the proportion ...the real-time PCR assay yielded a sensitivity of ...100%. Real-time PCR is a very rapid method, and it can ... See full document
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Detection of a Point Mutation Associated with High Level Isoniazid Resistance in Mycobacterium tuberculosis by Using Real Time PCR Technology with 3′ Minor Groove Binder DNA Probes
... of Mycobacterium tuberculosis is a serious public health ...M. tuberculosis and is mainly caused by mutations in the catalase peroxidase gene ...single-tube real-time PCR, using a novel ... See full document
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Novel Technique of Quantitative Nested Real Time PCR Assay for Mycobacterium tuberculosis DNA
... of Mycobacterium tuberculosis DNA in cerebro- spinal fluid (CSF) samples using PCR has been widely used as a more rapid, sensitive, and specific diagnostic method than the conventional bacteriological ... See full document
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Evaluation of Role of Xpert MTB/RIF in Diagnosis of Extra Pulmonary Tuberculosis in Patients attending Tertiary Care Hospital
... National Tuberculosis Programme in developing ...nested real time PCR and molecular beacon technology with a sensitivity of detecting 131 cfu /ml of Mycobacterium tuberculosis in sputum ... See full document
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Direct Detection of Rifampin Resistant Mycobacterium tuberculosis in Respiratory Specimens by PCR DNA Sequencing
... and sequencing, respiratory specimens should be prioritized rather than tested ...recurrent tuberculosis (18), previ- ously treated cases (8), treatment default (14), irregular prior treatment (15), and ... See full document
6
Rapid Detection of Mycobacterium tuberculosis Beijing Genotype Strains by Real Time PCR
... the real-time PCR machine is able simultaneously to measure multiple dyes with distinct emission wavelengths, a multiplex PCR assay applying both PCRs in one reaction was ... See full document
6
Multiplex Real Time PCR shortTUB Assay for Detection of the Mycobacterium tuberculosis Complex in Smear Negative Clinical Samples with Low Mycobacterial Loads
... ABSTRACT Tuberculosis (TB) remains a major health problem ...the Mycobacterium tuberculosis complex (MTBC) have emerged in recent ...years. Real-time PCR amplifica- tion simultaneously ... See full document
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Clinical Evaluation of TRCRapid M TB for Detection of Mycobacterium tuberculosis Complex in Respiratory and Nonrespiratory Specimens
... TRCRapid-160 real-time monitor (Tosoh Bioscience), for 5 min while it was being preheated to 43°C, followed by addition of 5 l of the enzyme ...TRCRapid-160 real-time monitor consists of a ... See full document
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A single nucleotide polymorphism real time PCR assay for genotyping of Mycobacterium tuberculosis complex in peri urban Kampala
... Abbreviation MTBC: Mycobacterium tuberculosis complex; TB: Tuberculosis; Tm: Melting temperature; SNP: ingle nucleotide polymorphism; RT-PCR: Real time polymerase chain reaction; LSP-PCR[r] ... See full document
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Deciphering drug resistance in Mycobacterium tuberculosis using whole-genome sequencing: progress, promise, and challenges
... M. tuberculosis de- velops and compensates for drug ...the time, even for distinct global regions and time frames, includ- ing times when both rifampicin and isoniazid were in use, suggesting that ... See full document
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Detection and Differentiation of Mycobacterium tuberculosis and Nontuberculous Mycobacterial Isolates by Real Time PCR
... M. tuberculosis complex from other mycobacteria (12, 13, ...M. tuberculosis or require additional hybridization probe sets to detect other mycobacteria (12, 13, ...M. tuberculosis and distinguished ... See full document
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Novel Multiplex Real Time PCR Diagnostic Assay for Identification and Differentiation of Mycobacterium tuberculosis, Mycobacterium canettii, and Mycobacterium tuberculosis Complex Strains
... available real-time PCR kits for the diagnosis of TB generally identify the MTC but not individual ...M. tuberculosis and ...M. tuberculosis and have been used for the differentiation of ... See full document
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