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Recombination between Sindbis virus RNAs.
... The sequence of the recombinant RNAs and the analysis of the viral RNAs in infected cells indicated that those recombinants with two subgenomic RNA promoters did not arise by a simple cr[r] ... See full document
9
Sindbis Virus with a Tricomponent Genome
... interference between replicating helper RNAs and replicons expressing the genes of interest in the infected ...helper RNAs capable of efficient replication had only a minor effect on GFP ... See full document
7
Sindbis virus replicons and Sindbis virus: assembly of chimeras and of particles deficient in virus RNA.
... mosaic virus (6) and turnip crinkle virus ...of virus-like particles, demonstrating that the capsid proteins with deletions were able to undergo capsid-capsid interactions and interact with the ... See full document
11
Recombination ready Sindbis replicon expression vectors for transgene expression
... structural proteins that form virus particles. The lack of intercellular spread by the replicons allows for the isola- tion of clonal cell populations that express a gene of inter- est, which is useful if the ... See full document
11
Identification of RNase L Dependent, 3′ End Modified, Viral Small RNAs in Sindbis Virus Infected Mammalian Cells
... small RNAs by knocking down Drosha, Dicer, and ...of Sindbis viral small RNAs detected by Northern blot analysis; this is accompanied by an upregulation of the viral genomic ...Nile virus ... See full document
10
Evidence for specificity in the encapsidation of Sindbis virus RNAs.
... 5310 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest We investigated the interaction of the capsid protein of Sindbis virus with Sindbis viral RNAs and defined a region[r] ... See full document
9
Genetic determinants of Sindbis virus neuroinvasiveness.
... mice, Sindbis virus replicates in a variety of tissues, leading to ...the virus can enter the central nervous system (CNS) and cause lethal ...and virus strain dependent. Recently, two pairs ... See full document
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Nonhomologous RNA-RNA recombination events at the 3' nontranslated region of the Sindbis virus genome: hot spots and utilization of nonviral sequences.
... The virus titers and plaque sizes were determined on Vero ...of virus were produced from cells transfected with combination RNA ...that recombination be- tween these precursors occurred at even lower ... See full document
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In vivo restoration of biologically active 3' ends of virus-associated RNAs by nonhomologous RNA recombination and replacement of a terminal motif.
... gous recombination (recombination between unrelated RNAs), such as that found associated with turnip crinkle virus (TCV), is less ...system, recombination occurs between ... See full document
9
RNA-RNA recombination in Sindbis virus: roles of the 3' conserved motif, poly(A) tail, and nonviral sequences of template RNAs in polymerase recognition and template switching.
... released virus was recovered (Table 2, experi- ment ...subgenomic RNAs were ex- pressed from each of the recombinants (data not ...on recombination. All of the recombination studies on the ... See full document
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Common and distinct regions of defective-interfering RNAs of Sindbis virus.
... These 3' and 5' conserved sequences were the only regions of nucleotide sequence homology we detected between the Sindbis virus-derived DI RNA and the DI RNAs derived from Semliki Forest[r] ... See full document
8
Evolution of virus and defective-interfering RNAs in BHK cells persistently infected with Sindbis virus.
... Electrophoretic analysis of glyoxal-denational high-multiplicity passaging did not yield a tured RNAs obtained from BHK cells persistently source of DI particles that were capable of inf[r] ... See full document
9
Viral RNAs Associated with Ribosomes in Sindbis Virus-Infected HeLa Cells
... Such complexes should have sedimentation rates only slightly higher than the monosomes, since Vesco and Colombo 45 established that HeLa 399 Downloaded from http://jvi.asm.org/ on Novemb[r] ... See full document
17
Genesis of Sindbis virus by in vivo recombination of nonreplicative RNA precursors.
... binant virus-infected cells (42); (iv) characteristic plaque for- mation by recombinant viruses ...the RNAs were purified by phenol- chloroform extraction before ...any virus or SIN-specific ... See full document
11
Effects of 5'-terminal modifications on the biological activity of defective interfering RNAs of Sindbis virus.
... These plasmids have the same DI cDNA sequences except that at the 5' terminus KDI-25 has nucleotides 10 to 75 of tRNAASP and KDI-549 has the 5'-terminal sequence of Sindbis virion 49S RN[r] ... See full document
7
Studies of defective interfering RNAs of Sindbis virus with and without tRNAAsp sequences at their 5' termini.
... Our finding that one-half of the DI RNAs generated and three of four of those generated in chicken embryo fibroblasts had tRNAASP sequences at their 5' termini suggests that these sequen[r] ... See full document
7
Sindbis virus expression vectors: packaging of RNA replicons by using defective helper RNAs.
... Our results with packaged Sindbis virus replicon particles indicate that even in those examples in which the viral structural proteins were not detected, low amounts of helper RNAs may b[r] ... See full document
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In Vitro Synthesis of Sindbis Virus Genomic and Subgenomic RNAs: Influence of nsP4 Mutations and Nucleoside Triphosphate Concentrations
... in Sindbis virus-infected cells, the genomic (G) RNA and the subgenomic (SG) ...(wt) Sindbis virus, the latter is made in excess over the former; however, in cells infected with SVpzf or SVcpc ... See full document
8
Expression of Sindbis virus structural proteins via recombinant vaccinia virus: synthesis, processing, and incorporation into mature Sindbis virions.
... To determine what protein products were translated in vivo from the Sindbis-specific transcripts, BSC-40 cells infected with wild-type vaccinia virus, vaccinia recombinant VV3S-7, or Sin[r] ... See full document
13
Role of the Vacuolar-ATPase in Sindbis Virus Infection
... It was shown that during SV infection, the effects of BAF were reversible when used at RT (Table 1). SV infection has been shown to be efficient at RT; SV RNA penetrated the cell and was therefore protected from ... See full document
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