[PDF] Top 20 Reflections on the biology of embryonic stem (ES) cells

... Int J D BioI 41 235 243 (1997) 235 Reflections on the biology of embryonic stem (ES) cells RICHARD L GARDNER' and FRANCES A BROOK Department of Zoology, University of Oxford, Oxford, United Kingdom AB[.] ... See full document

... mammalian stem cells. Although mouse and human embryonic stem (ES) cells are both derived from the pluripotent inner cell mass (ICM) cells of the pre-implantation ... See full document

... human embryonic stem (hES) cells in ...hES cells, transcriptomes of supportive (mouse fibroblasts derived from ...of ES cells in ...hES cells in ... See full document

... expands ES cell–derived megakaryocyte precursors that give rise to functional platelets after dox withdrawal ...G1ME2 cells (not ...blood cells or mega- karyocytes/platelets for transfusion ... See full document

... cultured cells has a number o f advantages because they are not only easy to manipulate in culture but can also be maintained in the differentiated or the undifferentiated ...(EC) cells are transformed cell ... See full document

... for stem cell ...diploid ES cells [14-16] from fertilization midblastula embryos as the equivalent of mammalian blastocysts, male germ stem cells from the adult testis [17] and even ... See full document

... mouse embryonic stem (ES) cells were investigated in order to evaluate the ES cell test (EST) used in a European Union validation study for screening of embryotoxic agents in ...of ... See full document

... In many instances, it is possible to express foreign genes under the control of the viral promoter, but in embryonal carcinoma EC cells, embryonic stem ES cells, and the preimplantation [r] ... See full document

... early embryonic cells and a stepwise acquisition of information gradually restricts this potency and establishes epigenetic barriers to avoid transition from one state to ...where cells in the ICM ... See full document

... 129Sv/J embryonic stem (ES) ...EcoRV-digested ES cell DNA. Confirmed ES clone 271 harboring the conditional Tead4 allele was microinjected into C57Bl/6 blastocysts, and blastocysts were ... See full document

... Blm-deficient embryonic stem cells were exposed to the plant toxin, ...resistant cells revealed an increase in the levels of hybrid glycan structures and a reduction in terminal b-galactose ... See full document

... and embryonic carcinoma cells are known for their ability to potently restrict retroviral expression [7,8], which involves two ...in ES cells using murine stem cell virus (MSCV) ... See full document

... nuclear cells, it is possible to obtain MSCs known to give rise to limb-bud mesoderm, at the single-cell level, that also differentiate into cells of visceral mesoderm and can be expanded extensively by ... See full document

... neural stem cells [1], embryonic stem (ES) cell derivatives [2,3], and adult bone marrow stromal cells (BMSCs) [4-6], have been transplanted into the injured spinal cord of rats ... See full document

... ES cells are derived from the inner cell mass (ICM) of pre-implantation stage blastocysts and can give rise, in vivo, to all three germ layers including the germ-line, as shown in mice by blastocyst ... See full document

... placed stem cell research at center stage in cardiology. A stem cell is a cell that has the ability to divide (self replicate) for indefinite periods often throughout the life of the ...with ... See full document

... “putative stem cells ” generated adherent oocyte-like cells, 20-95 μ m in diameter, with ZP-like, germinal vesicle-like and polar body-like structures that were ascribed to an oocyte ...from ... See full document

... Demethylation accompanied by de novo methylation occurs in gametogenesis, this is mediated by the DNA methyltransferases DNMT3a and DNMT3b, this process is essential for the establishment of parental imprinting of ... See full document

... murine embryonic stem cell clones ...technique. Embryonic lethality, affecting about a third of the knockouts generated, is the most prominent problem which prevents the study of the targeted gene, ... See full document

... of cells from G2 to mitosis (M) phase during cell division, this provides an additional level of cell cycle regulation in the p53-mediated ...eukaryotic cells with pleiotrophic effects on a wide variety of ... See full document