[PDF] Top 20 Repetitive Sequence Based PCR Using the DiversiLab System for Identification of Aspergillus Species
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Repetitive Sequence Based PCR Using the DiversiLab System for Identification of Aspergillus Species
... the species level based on conventional testing (colony morphology and microscopic characteristics), and 5 ATCC strains ...encountered species, and for ...the species name and a code number, ... See full document
5
Identification of Histoplasma capsulatum, Blastomyces dermatitidis, and Coccidioides Species by Repetitive Sequence Based PCR
... automated repetitive-sequence-based PCR (rep-PCR) using the Diversi- Lab system (Bacterial Barcodes, ...encountered Aspergillus species (5), Fusarium ... See full document
6
Identification to the Species Level and Differentiation between Strains of Aspergillus Clinical Isolates by Automated Repetitive Sequence Based PCR
... gal species. The DiversiLab Aspergillus kit was easy to use, and the automated detection and analysis by the DiversiLab system provided readily interpretable ...the system uses a ... See full document
9
Repetitive Sequence PCR Based DNA Fingerprinting Using the DiversiLab System for Identification of Commonly Encountered Dermatophytes
... of repetitive-sequence-based PCR (rep-PCR) using the DiversiLab system for identification of dermatophytes commonly isolated in a clinical laboratory was ... See full document
7
Comparison of the DiversiLab Repetitive Element PCR System with spa Typing and Pulsed Field Gel Electrophoresis for Clonal Characterization of Methicillin Resistant Staphylococcus aureus
... analysis using pulsed-field gel elec- trophoresis (PFGE) was one of the first genome-based typing methods for MRSA (2), followed by repetitive element PCR typing (rep-PCR) (6), spa ... See full document
7
Clonal Relationship and Differentiation among Mycobacterium abscessus Isolates as Determined Using the Semiautomated Repetitive Extragenic Palindromic Sequence PCR Based DiversiLab System
... The good performance we obtained for the DL REP-PCR test for genotyping M. abscessus isolates relies on high reproducibility. This was obtained only because we followed precisely the condi- tions required by the ... See full document
9
Evaluation of a High Throughput Repetitive Sequence Based PCR System for DNA Fingerprinting of Mycobacterium tuberculosis and Mycobacterium avium Complex Strains
... available repetitive-sequence-based PCR (rep-PCR) system was adapted for use on ...of repetitive elements interspersed throughout bacterial ...by PCR, these ... See full document
9
Rapid Detection and Identification ofCandida, Aspergillus, and FusariumSpecies in Ocular Samples Using Nested PCR
... three species, Candida albicans, Aspergillus fumigatus, and Fusarium solani, has been ...three species were designed. Panfungal PCR was followed by three nested PCRs utilizing ... See full document
7
Comparison of a Semiautomated Commercial Repetitive Sequence Based PCR Method with Spoligotyping, 24 Locus Mycobacterial Interspersed Repetitive Unit–Variable Number Tandem Repeat Typing, and Restriction Fragment Length Polymorphism Based Analysis of IS6110 for Mycobacterium tuberculosis Typing
... Repetitive-sequence-based PCR (rep-PCR) is a rapid typing pro- cedure using primers that bind to multiple noncoding repetitive se- quences interspersed throughout the ... See full document
5
Controlled Performance Evaluation of the DiversiLab Repetitive Sequence Based Genotyping System for Typing Multidrug Resistant Health Care Associated Bacterial Pathogens
... DL system is a rapid and semiautomated rep-PCR commercial system that uses standard PCR kits and high-resolution ampli- con detection by microfluidic capillary ...DL system has shown a ... See full document
5
Genomic fingerprinting of Bartonella species by repetitive element PCR for distinguishing species and isolates
... Repetitive-element PCR (rep-PCR) with primers based on repetitive extragenic palindromic (REP) and enterobacterial repetitive intergenic consensus (ERIC) repeated DNA sequences ... See full document
5
Rapid identification and fingerprinting of Candida krusei by PCR based amplification of the species specific repetitive polymorphic sequence CKRS 1
... the PCR products were analyzed on a 1% agarose gel (type II medium EEO; Sigma Chemical ...for PCR identification and fingerprinting of ...indicate repetitive sequences (a, b, c, and CKRS-1) in ... See full document
7
Microbial DNA Typing by Automated Repetitive Sequence Based PCR
... siLab system has the potential to be the standardized platform for routine bacterial and fungal genotyping in the clinical lab- ...The DiversiLab system offers efficiency, excellent dis- criminatory ... See full document
9
Comparative Evaluation of an Automated Repetitive Sequence Based PCR Instrument versus Pulsed Field Gel Electrophoresis in the Setting of a Serratia marcescens Nosocomial Infection Outbreak
... colony using an UltraClean Microbial DNA isolation kit (Mo Bio Laboratories, Carlsbad, CA) and following the manufac- turer’s ...amplified using a DiversiLab Ser- ratia DNA fingerprinting kit (bioMe ... See full document
6
Clinical Evaluation of the DiversiLab Microbial Typing System Using Repetitive Sequence Based PCR for Characterization of Staphylococcus aureus Strains
... automated DiversiLab sys- tem (Spectral Genomics, ...rep- PCR to discriminate among bacterial and fungal isolates and to identify some fungi to the species level ...noncoding, repetitive ... See full document
6
Improving the PCR protocol to amplify a repetitive DNA sequence.
... Two more primer pairs (Table 1) were used to test the efficacy of the new program, and to test the effects of varying annealing sites on the amplification of the repetitive fragment, as reported by Hommelsheim et ... See full document
11
Molecular diagnosis of cutaneous leishmaniasis and identification of the causative Leishmania species in Morocco by using three PCR based assays
... mainly based on clinical symptoms and microscopic examin- ation of smears; culture is carried out only in some spe- cialized ...Leishmania species to- gether with the clinical presentation of the resulting ... See full document
9
Development of multiplex real-time PCR assays for identification of members of the Anopheles funestus species group
... target species of the An. funestus group from the melting temperature of the PCR amplicons which ranged in size from 146 to 587 ...plots using the associated Rotor-Gene Software (version ...each ... See full document
10
Application of ubiquinone systems and electrophoretic comparison of enzymes to identification of clinical isolates of Aspergillus fumigatus and several other species of Aspergillus
... Application of Ubiquinone Systems and Electrophoretic Comparison of Enzymes to Identification of Clinical Isolates of Aspergillus fumigatus and Several Other Species of Aspergillus.. The[r] ... See full document
7
Efficacy of Pulsed Field Gel Electrophoresis and Repetitive Element Sequence Based PCR in Typing of Salmonella Isolates from Assam, India
... In the PFGE analysis, the banding patterns revealed a total of 30 distinct polymor- phic bands, which ranged from 22 to 1,135 bp in size (Fig. 1). The bands of 22 bp and 249 bp were shared by all the isolates, while ... See full document
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