[PDF] Top 20 A replication function associated with the activation domain of the Epstein-Barr virus Zta transactivator.

... a replication function within the Zta trans- activation ...four Zta variants possessing deletions in the Zta activation domain for their effects on ori-Lyt ... See full document

... Epstein-Barr virus (EBV) immediate-early protein Zta is a member of the basic-leucine zipper (B-ZIP) family of DNA binding proteins that has an unusual capacity to recognize multiple DNA ... See full document

... the Zta activation ...of Zta resulted in translocation of BBLF4-GFP into the nucleus indicating interaction between these two ...However, Zta with a deletion of aa 24 to 86 was unable to ... See full document

... lytic replication, but its mechanism of action is also unknown (47, ...Both Zta and K8 share the ability to interact with C/EBP, and C/EBP binding sites can be found throughout the KSHV OriLyt (47, 71, ... See full document

... another virus, or the expres- sion of the EBV ZEBRA protein (BamHI Z Epstein-Barr replication activator; also known as EB1 and Zta) ...DNA replication (16, ... See full document

... Epstein-Barr virus (EBV), a human herpesvirus, infects lym- phoid and epithelial ...this virus is closely related to several neoplastic diseases, including Burkitt’s lym- phoma, T-cell ... See full document

... (nuclear domain 10) are discrete nuclear protein aggregations characterized by a set of interferon-upregulated proteins including Sp100 and PML, where papova-, adeno-, and herpesvi- ruses begin their transcription ... See full document

... is Zta, en- coded by the BZLF1 open reading frame (ORF) (13). Zta is a bZIP transcriptional activator with a unique non-leucine-based dimerization domain (9, 26, ...27). Zta binds directly as ... See full document

... LF2 inhibits activation by Rta fused to a heterologous DBD. Because LF2 induces Rta SUMOylation, its repressive effects may extend beyond inhibition of DNA binding. In order to test this possibility, we cloned Rta ... See full document

... is associated with cell cycle arrest (3, 4, 15, 18, 21–23, 27), and it is assumed that this supports an environment for more- robust viral ...detectable Zta levels (22). It has also been found that ... See full document

... of Zta, in vivo protein association assays were carried out in two cell systems containing EBV that were permissive to lytic re- ...of Zta, pCDNA3HisZta, to induce lytic cycle reactiva- ...isolate ... See full document

... been associated with a broad range of biologic functions such as cell prolifer- ation (3, 4, 41), apoptosis (39, 51, 54), and differentiation (28, 50) in a cell-type-dependent ...and activation of a ternary ... See full document

... primary replication-specific function for Zta in the infected cell since the tripartite helicase-primase complex is itself capable of nuclear ...If Zta were to fulfill such a role, then the ... See full document

... by Zta for reactivation of la- tent ...of Zta to stim- ulate viral gene expression in latently infected D98/HR1 ...cells. Zta alanine substitution mutants were transfected into D98/ HR1 cells and ... See full document

... Epstein-Barr virus (EBV) is a ubiquitous human gammaher- ...the virus is able to immortalize B cells in vivo and in vitro, and it is associated with three human malignancies, Burkitt’s ... See full document

... ing domain of DnaA identified two amino acids, K178 and N235, as crucial residues for the protein’s affinity for ATP or ...bacterial replication 10-fold and inhibited opening of the DNA duplex at oriC ... See full document

... gene function; column 5, previous class assignment (U, unknown; IE, immediate-early; E, early; L, late; LT, latent); column 6, doubling time (DT); column 7, peak time (PT); columns 8 to 16, activation ... See full document

... The region of the EBV genome containing the rightward divergent promoter and the EA-R gene pMH53 was cotransfected with BamHI-Z under the regulation of the SV40 promoter pPL17 A, HindIII[r] ... See full document

... immunoglobulin, virus super- infection, and treatment with phorbol ester, 5-azacytidine, n- butyrate, or histone deacetylase inhibitors (4, 10, 13, 62, 66, ...and Zta (21, 57, ... See full document

... How does p53 regulate the activity of EBV Zp? One possi- bility is that p53 binds directly to a responsive element in the Zp region. However, according to sequence analysis, there is no consensus p53-binding site present ... See full document