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Replication of human hepatitis delta virus in primary cultures of woodchuck hepatocytes.
... 2891 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest We obtained two lines of evidence that monolayer cultures of primary woodchuck hepatocytes support replication of t[r] ... See full document
5
Lack of effect of antiviral therapy in nondividing hepatocyte cultures on the closed circular DNA of woodchuck hepatitis virus.
... infecting virus (see Fig. 4B). Thus, the primary cultures of woodchuck hepatocytes appeared to support a complete cycle of WHV replication, from infection to progeny virus ... See full document
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Woodchuck hepatitis virus X protein is present in chronically infected woodchuck liver and woodchuck hepatocellular carcinomas which are permissive for viral replication.
... in primary hepatocytes, we fractionated hepatocytes into crude cytoplasmic and nuclear ...of woodchuck hepatocyte nu- clei eliminated any detectable WHx protein ... See full document
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Replication of the Human Hepatitis Delta Virus Genome Is Initiated in Mouse Hepatocytes following Intravenous Injection of Naked DNA or RNA Sequences
... series of mice were transfected with pDL456, a construct based on the vector pSVL (Pharmacia) with an insert of 1.2⫻ unit-length HDV cDNA, which uses the simian virus 40 promoter to express RNA that is antigenomic ... See full document
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Spatiotemporal Analysis of Hepatitis B Virus X Protein in Primary Human Hepatocytes
... HBV replication endpoints nor a decrease in intracellular HBx levels is a good predictor of HBx functional inhibition by siRNA ...HBV-infected hepatocytes is the most important criterion for identifying ... See full document
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Genomic responses to hepatitis B virus (HBV) infection in primary human hepatocytes
... Raw methylation data was imported and processed using R/Bioconductor packages [37, 38]. Data quality was inspected using boxplots for the distribution of methylated and unmethylated signals, and inter-sample relationship ... See full document
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Initiation of Hepatitis Delta Virus Genome Replication
... the virus binds to a susceptible cell via an as yet unidentified receptor, is inter- nalized, uncoated, and transported to the nucleus, and then RNA-directed RNA synthesis is somehow ...with cultures of ... See full document
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Recombinant Duck Interferon Gamma Inhibits Duck Hepatitis B Virus Replication in Primary Hepatocytes
... the human hepatitis B virus (HBV), the woodchuck hepatitis virus (WHV), and the duck hepatitis B virus ...viral replication and gene expression and ... See full document
7
Entry of Hepatitis B Virus into Immortalized Human Primary Hepatocytes by Clathrin-Dependent Endocytosis
... in primary cultures (17, ...gene replication by reverse transcrip- tion-PCR ...in virus-infected DMSO-treated HuS-E/2 cells, total DNA was extracted from the cells and analyzed for the ... See full document
11
Limitations to Replication of Hepatitis Delta Virus in Avian Cells
... Human hepatitis delta virus (HDV) is a natural subviral agent that uses hepatitis B virus as a ...using woodchuck hepatitis B virus as a helper ...activity, ... See full document
6
Primary Human Hepatocytes Are Susceptible to Infection by Hepatitis Delta Virus Assembled with Envelope Proteins of Woodchuck Hepatitis Virus
... into primary hepatocytes, we made use of a panel of six pep- tides bearing pre-S sequences, with three derived from HBV L and three from WHV L, as summarized in Table ...the virus for binding of the ... See full document
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Serum-Derived Hepatitis C Virus Infection of Primary Human Hepatocytes Is Tetraspanin CD81 Dependent
... infect primary human hepatocytes as revealed here by the intracel- lular accumulation of both genomic and replicative strands (not ...mary hepatocytes, it has to be noted that less than 15% of ... See full document
6
Efficient Infection of Primary Tupaia Hepatocytes with Purified Human and Woolly Monkey Hepatitis B Virus
... worldwide, hepatitis B virus (HBV) infection represents a ma- jor health care ...viral replication in only about one-third of patients, and lamivudine resistance with mutations in the viral ... See full document
6
Vectorial Release of Hepatitis E Virus in Polarized Human Hepatocytes
... like hepatitis A virus (HAV), hepatitis B virus (HBV), and hepatitis C virus (HCV) use hepatocyte polarization for their release ...naked virus that is lipid associated in ... See full document
14
Assembly of Hepatitis B Virus Envelope Proteins onto a Lentivirus Pseudotype That Infects Primary Human Hepatocytes
... of hepatitis B virus (HBV) could be incorporated into the lipid membrane of lentivirus pseudotype ...a human immunodeficiency virus (HIV) packaging construct, (ii) a transfer plasmid ... See full document
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Transcription of Hepatitis Delta Antigen mRNA Continues throughout Hepatitis Delta Virus (HDV) Replication: a New Model of HDV RNA Transcription and Replication
... HDV-infected human or chimpanzee liver tis- sues or in cell cultures and transgenic mice (24) which actively replicate HDV ...RNA replication has always been studied by using an HDV cDNA transfection ... See full document
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Efficient inhibition of hepatitis B virus replication by hepatitis delta virus ribozymes delivered by targeting retrovirus
... Background: Hepatitis delta virus (HDV) ribozyme is an attractive molecular tool that can specifically recognize and catalyze the self-cleavage of the viral RNA phosphodiester ...in hepatitis ... See full document
6
Assembly of Hepatitis Delta Virus: Particle Characterization, Including the Ability To Infect Primary Human Hepatocytes
... Rate-zonal sedimentation. Several sources of virus were used for Fig. 3. For Fig. 3A and B, the HDV was from media harvested between days 6 to 9 and 6 to 12, respectively, after transfection with pSVL(D3) and ... See full document
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Replication of Naturally Occurring Woodchuck Hepatitis Virus Deletion Mutants in Primary Hepatocyte Cultures and after Transmission to Naive Woodchucks
... Woodchuck hepatitis virus (WHV) mutants with core internal deletions (CID) occur naturally in chronically WHV-infected woodchucks, as do hepatitis B virus mutants in ...the ... See full document
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Woodchuck Gamma Interferon Upregulates Major Histocompatibility Complex Class I Transcription but Is Unable To Deplete Woodchuck Hepatitis Virus Replication Intermediates and RNAs in Persistently Infected Woodchuck Primary Hepatocytes
... intrahepatic replication intermediates and specific mRNAs of hepatitis B virus (HBV) by a noncytolytic mechanism in the transgenic mouse ...recombinant woodchuck IFN- ␥ (wIFN- ␥ ) in ... See full document
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