[PDF] Top 20 cis Requirement for N-specific protein sequence in bovine coronavirus defective interfering RNA replication.
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cis Requirement for N-specific protein sequence in bovine coronavirus defective interfering RNA replication.
... DI RNA replication (4, 10, 21). The differences in DI RNA behavior, however, are clear in three basic ...entire N gene sequence is present in the naturally occurring BCV DI RNA ... See full document
7
Bovine Coronavirus Nonstructural Protein 1 (p28) Is an RNA Binding Protein That Binds Terminal Genomic cis-Replication Elements
... DI RNA leads to inhibition of DI RNA accumulation but to only slight transient inhibition of viral ...BCoV replication, as might be expected of a regula- tory protein, pDrep-ISnsp1 was ... See full document
11
Translation but not the encoded sequence is essential for the efficient propagation of the defective interfering RNAs of the coronavirus mouse hepatitis virus.
... DI RNA- specific polypeptide migrates as a 88-kDa molecule ...DI RNA- transfected and MHV-infected cells or in the resulting p0 virus ...for RNA replication or protection against ... See full document
8
Host Protein Interactions with the 3′ End of Bovine Coronavirus RNA and the Requirement of the Poly(A) Tail for Coronavirus Defective Genome Replication
... in coronavirus defective genome ...important cis-acting signal for RNA replication. A BCV defective RNA replicon that lacked a poly(A) tail was not competent for ... See full document
13
Assembled coronavirus from complementation of two defective interfering RNAs.
... on RNA viruses other than retro- viruses have been developed previously ...in replication and assembly of Sindbis virus containing the foreign gene ...MHV sequence express these genes in DI ... See full document
10
Efficient Homologous RNA Recombination and Requirement for an Open Reading Frame during Replication of Equine Arteritis Virus Defective Interfering RNAs
... EDI RNA consists of three noncontiguous parts of the EAV genome ...leader sequence and the 5⬘-terminal ...EDI RNA contains a truncated replicase gene, which we will refer to as EDI-ORF, and encodes a ... See full document
9
The UCUAAAC promoter motif is not required for high-frequency leader recombination in bovine coronavirus defective interfering RNA.
... UCUAAAC sequence and thus downstream of the leader, a pattern that would fit a process of strand switching during minus-strand synthesis ...AUAAA sequence which prevented recombination and repli- cation ... See full document
10
Stem-Loop IV in the 5′ Untranslated Region Is a cis-Acting Element in Bovine Coronavirus Defective Interfering RNA Replication
... IV sequence are necessary for BCoV DI RNA ...primary sequence within group 2 coronaviruses ...DI RNA replica- ...and sequence analyses showed only wt in VP1 progeny, indicating mutant ... See full document
13
The 3' untranslated region of coronavirus RNA is required for subgenomic mRNA transcription from a defective interfering RNA.
... genomic RNA contains a recognition sequence (55 nucleotides [nt]) required for minus-strand RNA ...-end sequence is also involved in subgenomic mRNA transcription, we have constructed MHV ... See full document
5
cis-Acting Sequences Required for Coronavirus Infectious Bronchitis Virus Defective-RNA Replication and Packaging
... IBV sequence, were replicated, but overall rescue was ...any coronavirus D-RNA so far success- fully ...IBV sequence corresponding to the struc- tural protein genes did not improve ... See full document
9
Importance of the Positive-Strand RNA Secondary Structure of a Murine Coronavirus Defective Interfering RNA Internal Replication Signal in Positive-Strand RNA Synthesis
... single-strand RNA bacteriophage Qb contains two internal viral polymerase rec- ognition sites for negative-strand RNA synthesis; each internal recognition site is separated from its functional initiation ... See full document
8
An RNA Stem-Loop within the Bovine Coronavirus nsp1 Coding Region Is a cis-Acting Element in Defective Interfering RNA Replication
... the coronavirus common GGAAGAGC octamer sequence and an adjacent helical region ...The cis-acting functions of the 3 ⬘ UTR elements may be common among the group 2 coronaviruses, since the BCoV DI ... See full document
9
Characterization of a murine coronavirus defective interfering RNA internal cis-acting replication signal.
... positive-strand RNA synthesis but not for negative-strand RNA ...-end sequence of MHV genomic RNA is sufficient for MHV negative-strand RNA synthesis ...positive-strand RNA ... See full document
9
A cis-acting function for the coronavirus leader in defective interfering RNA replication.
... To directly test the hypothesis that the leader is sufficient as a 5'-terminal signal for coronavirus RNA replication 18, 43, a clone of BCV N mRNA 40 with a precise 5'-terminal sequence[r] ... See full document
9
Isolation and Characterization of an Arterivirus Defective Interfering RNA Genome
... a defective arterivirus genome. A DI RNA of approximately 8 kb (DI-a) was readily observed in P6 of our serial passaging experiment, but the 6-kb DI-b RNA used in this study surfaced only after 21 ... See full document
10
Defective interfering passages of Sindbis virus: nature of the defective virion RNA.
... Bruton and Kennedy found that the 20S RNA isolated from DI particles of Semliki forest virus and also obtained from BHK cells infected with both standard and defective Semliki forest vir[r] ... See full document
10
Requirement of the 5'-end genomic sequence as an upstream cis-acting element for coronavirus subgenomic mRNA transcription.
... The requirement of gene 1 and 5' UTR sequences as spacer sequences for subgenomic mRNA transcription strongly suggests that the wild-type viral subgenomic mRNAs mRNA 2 to mRNA 7 and thei[r] ... See full document
11
Specific interaction between coronavirus leader RNA and nucleocapsid protein.
... To confirm the direct binding of N protein to the sequences contained within the leader RNA, we examined in vitro transcripts prepared from a pT7 plasmid containing an insert composed of[r] ... See full document
8
Sequence analysis of cDNA's derived from the RNA of Sindbis virions and of defective interfering particles.
... This 3'-terminal conserved sequence was also found in two DI RNA populations of Sindbis virus and two variants of this virus obtained from persistently infected BHK cells, supporting the[r] ... See full document
10
In vivo accumulation of a turnip crinkle virus defective interfering RNA is affected by alterations in size and sequence.
... Studies of the infectivity of DI transcripts containing deletions, insertions, and single-base changes suggest that i in general, only the 5' two-thirds of the molecule can tolerate muta[r] ... See full document
9
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