[PDF] Top 20 Screening and identification of B cell epitopes within envelope protein of tembusu virus
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Screening and identification of B cell epitopes within envelope protein of tembusu virus
... envelope protein (E) [7]. In mature virus, E protein forms homodimer in head-to-tail conformation and as- sembles dense lattice on the surface of virion ...E protein mediates ... See full document
8
In Silico Screening and Identification of Antiviral Inhibitors against Envelope Protein of Yellow Fever Virus
... Docking is an automated computer algorithm that determines how a compound will bind to the binding site of a protein. The approach includes verifying the orientation of the compound, its conformational geometry, ... See full document
5
Specificities of Human CD4+ T Cell Responses to an Inactivated Flavivirus Vaccine and Infection: Correlation with Structure and Epitope Prediction
... DEN virus particles, consists of three alpha helices that are located beneath the E pro- tein shell and interact with the viral membrane ...dicted epitopes), it is unlikely that the differences between ... See full document
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Identification of envelope protein epitopes that are important in the attenuation process of wild-type yellow fever virus.
... Virulence of MAb' variants and their parent viruses for newborn mice Virulence DISCUSSION In the work described in this paper, panels of MAbs have been prepared against vaccine and wild-[r] ... See full document
6
Identification of linear human B cell epitopes of tick borne encephalitis virus
... recombinant virus-like particles [15,16]. TBEV E-protein is highly cross-reactive among other members of the genus Flavivirus and thus the identification of TBEV-specific antigenic regions would be ... See full document
9
Identification of B-Cell Epitopes on Virus-Like Particles of Cutaneous Alpha-Human Papillomaviruses
... of virus-like particle (VLP)-based ...the B-cell epitopes on VLPs of cutaneous alpha-HPVs using a panel of 94 monoclonal antibodies (MAbs) generated upon immunization with capsids from HPV ... See full document
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Identification of a conserved JEV serocomplex B cell epitope by screening a phage display peptide library with a mAb generated against West Nile virus capsid protein
... Recombinant protein was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS- PAGE) and purificated by Ni-nitrilotriacetic acid affinity chromatography (Qiagen) according to the manufac- ... See full document
10
Characterization of Two Novel Linear B-Cell Epitopes in the Capsid Protein of Avian Hepatitis E Virus (HEV) That Are Common to Avian, Swine, and Human HEVs
... two epitopes with anti-avian, anti-swine, and anti-human HEV sera, positive serum samples from chickens challenged by geno- type 3 avian HEV, pigs infected by genotype 4 swine HEV, and humans infected by genotype ... See full document
11
Identification of T-cell epitopes on E2 protein of rubella virus, as recognized by human T-cell lines and clones.
... E2-4 peptide induced strong cytotoxic reactions of T-cell clone A9 to the MANN and EBV-BL AT cell targets but no responses to human cell lines MAJA, MST, WT-20, PRIESS, or Lg-38 with dif[r] ... See full document
6
Identification of four novel group specific bluetongue virus NS3 protein B cell epitopes
... Firstly, we crushed down the gel contained the purified HIS-BTV15-NS3 protein and acutely stirred till it pre- sented mushy state. Then, six-week-old female BALB/c mice were immunized subcutaneously with 100 μg ... See full document
11
A Single Mutation at Position 156 in the Envelope Protein of Tembusu Virus Is Responsible for Virus Tissue Tropism and Transmissibility in Ducks
... the protein in host- specific adaption, cell tropism, virus attachment, and membrane fusion with target cells (28, 34, 45, 47, 48, ...E protein also has three different structural domains ...E ... See full document
16
Identification of Immunodominant Neutralizing Epitopes on the Hemagglutinin Protein of Rinderpest Virus
... mediate virus attachment and penetration to host cells and play a vital role in induction of protective ...F protein can be the target of neutralizing antibodies (13), most of the protective immu- nity to ... See full document
6
Human T-lymphotropic virus type 1 peptides in chimeric and multivalent constructs with promiscuous T-cell epitopes enhance immunogenicity and overcome genetic restriction.
... overlapping B-cell and T-cell epitopes), were designed as chimeric and b -template peptides ...face envelope protein in direct and competitive ELISA, as well as in ... See full document
13
Antigenicity and Immunogenicity of Novel Chimeric Hepatitis B Surface Antigen Particles with Exposed Hepatitis C Virus Epitopes
... the cell culture fluid similar to that of the negative control ...delta virus antigen (L- HDAg) were ...HBV envelope proteins, with HBsAg-S being sufficient for packaging (3, ...corresponding ... See full document
12
Virosomes of hepatitis B virus envelope L proteins containing doxorubicin: synergistic enhancement of human liver-specific antitumor growth activity by radiotherapy
... carcinoma cell lines, Huh7 (RCB1366, RIKEN, Wako) and NuE (a gift from Professor Takushi Tadakuma, National Defense Medical College, Tokoro- zawa, Japan), and human colon carcinoma cell line WiDr (JCRB0224; ... See full document
14
Identification of B- and T-Cell Epitopes of BB, a Carrier Protein Derived from the G Protein of Streptococcus Strain G148
... carrier protein could be a drawback to their repeated use in human programs, given the fact that the majority of subjects have been vaccinated against tetanus in early ...carrier-specific B memory cells ... See full document
8
Clinical and epidemiological investigations of Acinetobacter genomospecies 3 in a neonatal intensive care unit
... A combination of typing methods must be used to discrim- inate between the Acinetobacter strains that are involved in outbreaks (9, 11). We used three typing methods. Cross-colo- nization or -infection was suggested when ... See full document
6
Oligomeric rearrangement of tick-borne encephalitis virus envelope proteins induced by an acidic pH.
... TBE virus at a mildly acidic pH results in an irreversible conformational change which can be monitored by the loss of reactivity with MAb i2 in a four-layer ELISA ...the protein E dimer-to-trimer tran- ... See full document
6
SCREENING AND EVALUATION OF ANTIGENIC B-CELL EPITOPES FOR THE SERODIAGNOSIS OF LYMPHATIC FILARIASIS
... predicted epitopes sequences were represented in a 3D structure consisting of alpha helices and beta strands, mapping and analysis of the spatial arrangement of the epitopes ...40 epitopes obtained ... See full document
6
Analysis of the human T-cell response to picornaviruses: identification of T-cell epitopes close to B-cell epitopes in poliovirus.
... Twenty-nine individuals responded to poliovirus type 3, coxsackievirus B3, and encephalomyocarditis virus by proliferation of T cells, and from such cultures, 130 virus-specific T-cell l[r] ... See full document
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