[PDF] Top 20 Sequence and structural determinants required for priming of plus-strand DNA synthesis by the human immunodeficiency virus type 1 polypurine tract.
Has 10000 "Sequence and structural determinants required for priming of plus-strand DNA synthesis by the human immunodeficiency virus type 1 polypurine tract." found on our website. Below are the top 20 most common "Sequence and structural determinants required for priming of plus-strand DNA synthesis by the human immunodeficiency virus type 1 polypurine tract.".
Sequence and structural determinants required for priming of plus-strand DNA synthesis by the human immunodeficiency virus type 1 polypurine tract.
... given sequence can serve as a primer, we selected a 15-nt sequence (alternating GA [Table 1]) from the R-Pu/D-Py class (25) and tested for primer activity with HIV-1 ...GA sequence was ... See full document
9
Human immunodeficiency virus type 1 nucleocapsid protein promotes efficient strand transfer and specific viral DNA synthesis by inhibiting TAR-dependent self-priming from minus-strand strong-stop DNA.
... the sequence of the plus-strand DNA ex- tension at the junction with the 39 terminus of (2) ...remaining sequence complementary to nt 521 to 538 in HIV-1 NL4.3 (1), 9 nt ... See full document
11
Compensatory Role of Human Immunodeficiency Virus Central Polypurine Tract Sequence in Kinetically Disrupted Reverse Transcription
... )- strand DNA synthesis from the cPPT RNA primer at the center of the HIV-1 genome can compensate for delayed pro- viral DNA synthesis induced by enzymatically defective RT ... See full document
5
Effect of Polypurine Tract (PPT) Mutations on Human Immunodeficiency Virus Type 1 Replication: a Virus with a Completely Randomized PPT Retains Low Infectivity
... introduced polypurine tract (PPT) mutations, which we had previously tested in an in vitro assay, into the viral clone NL4-3KFS ⌬ ...PPT sequence. Curiously, a mutation in which the entire PPT ... See full document
9
Effects of Mutations in the G Tract of the Human Immunodeficiency Virus Type 1 Polypurine Tract on Virus Replication and RNase H Cleavage
... RNA strand of an RNA-DNA ...a DNA-DNA ...both DNA-DNA and RNA-RNA duplexes, is simplified by the fact that the three duplexes have different structures in ...solution. ... See full document
10
Functional Central Polypurine Tract Provides Downstream Protection of the Human Immunodeficiency Virus Type 1 Genome from Editing by APOBEC3G and APOBEC3B
... central plus-strand discontinuity is therefore represented by an ⬃ 100-nucleotide overlap between the two segments, also known as the DNA “flap” (7, 10, 29, ...a DNA flap has been mainly ... See full document
6
Discontinuous plus-strand DNA synthesis in human immunodeficiency virus type 1-infected cells and in a partially reconstituted cell-free system.
... (iii) Plus-strand synthesis from non-PPT ...of plus-strand DNAs were detected with 59 ends at non-PPT sites on both the nef and pol ...of plus-strand synthesis, ... See full document
11
Human Immunodeficiency Virus Type 1 Central DNA Flap: Dynamic Terminal Product of Plus-Strand Displacement DNA Synthesis Catalyzed by Reverse Transcriptase Assisted by Nucleocapsid Protein
... canonical polypurine tracts (PPTs), which are resistant against the nucleolytic deg- radation activity of the reverse transcriptase (RT)-associated RNase H (37, 38, ...the plus-strand ... See full document
13
Human immunodeficiency virus type 1 nucleocapsid protein reduces reverse transcriptase pausing at a secondary structure near the murine leukemia virus polypurine tract.
... minus-strand DNA synthesis catalyzed by murine leukemia virus reverse transcrip- tase, we described a prominent pause site near the polypurine tract ...the polypurine ... See full document
11
Deletion of a Short, Untranslated Region Adjacent to the Polypurine Tract in Moloney Murine Leukemia Virus Leads to Formation of Aberrant 5′ Plus-Strand DNA Ends In Vivo
... mechanism, priming which starts 5⬘ to the PPT will generate plus-strand DNA with additional sequences attached to the U3 region ...of priming of several viruses in vitro. Point mu- ... See full document
10
Mutations in the Human Immunodeficiency Virus Type 1 Polypurine Tract (PPT) Reduce the Rate of PPT Cleavage and Plus-Strand DNA Synthesis
... G tract showed that these mutations also had a significant effect on the relative rates of utilization of the ...the plus-strand DNA transfers, ...of plus-strand DNA ... See full document
5
Determinants of the Establishment of Human Immunodeficiency Virus Type 1 Latency
... Histone modifications during HIV-1 latency establishment. Histone modifications, such as the generation of a repressive histone code, are key mechanisms for the control of cellular gene expression. Histone ... See full document
16
Vpr Is Required for Efficient Nef Expression from Unintegrated Human Immunodeficiency Virus Type 1 DNA
... However, our studies also indicate that a nontranscriptional component is involved in Vpr’s activity with integrase-defective HIV. Vpr is able to enhance expression, albeit at a lower level, from a SIN HIV-based vector ... See full document
9
Zinc Finger Structures in the Human Immunodeficiency Virus Type 1 Nucleocapsid Protein Facilitate Efficient Minus- and Plus-Strand Transfer
... viral DNA synthesis, making it possible for RT to copy structured elements in RNA and DNA templates (24, 36, 40, 42, 44, 48, ...minus- strand transfer (2, 8, 17, 18, 20, 36, 42, 58, 61, ... See full document
9
The Karyophilic Properties of Human Immunodeficiency Virus Type 1 Integrase Are Not Required for Nuclear Import of Proviral DNA
... As we previously reported (46), under nonreducing condi- tions an additional IN-specific, strongly reactive signal with an apparent molecular mass of 70 kDa was observed in WT viri- ons, confirming that disulfide bridges ... See full document
8
The DNA Deaminase Activity of Human APOBEC3G Is Required for Ty1, MusD, and Human Immunodeficiency Virus Type 1 Restriction
... infections, virus stocks were produced by FuGene 6-medi- ated transfection of 5 g of HIV-1 provirus plasmid into 293T cells (10-cm dish, 50 to 70% ...incubation, virus-containing supernatants were ... See full document
9
Vif is crucial for human immunodeficiency virus type 1 proviral DNA synthesis in infected cells.
... Namalwa-CD4 cells are derivatives of a human B-cell line stably transfected with a CD4-expressing plasmid; they express high levels of cell surface CD4, are permissive for HIV-1 replicat[r] ... See full document
11
Pseudodiploid Genome Organization Aids Full-Length Human Immunodeficiency Virus Type 1 DNA Synthesis
... The results reported here suggest that like limiting the time available for reverse transcription by AZT treatment (Fig. 6), reverse transcription using a single template provides condi- tions that disfavor intact genome ... See full document
9
CD63 Is Not Required for Production of Infectious Human Immunodeficiency Virus Type 1 in Human Macrophages
... Although CD63-containing, tetraspanin-enriched domains appear to be essential sites for HIV assembly, both at the cell surface in T cells and tissue culture cell lines (25) and in the intracellular plasma ... See full document
11
Two DNA polymerases may be required for synthesis of the lagging DNA strand of simian virus 40.
... In contrast, the residual incorporation into the long chains that occurred in the presence of aphidicolin was expected to be confined to leading portions, since synthesis of Okazaki piec[r] ... See full document
7
Related subjects