[PDF] Top 20 Sequence Variations in Human Immunodeficiency Virus Type 1 Nef Are Associated with Different Stages of Disease

... that Nef is not a common mediator of the rate of HIV-1 disease ...defective nef genes in HIV-1 infection has been in- vestigated in only a relatively small number of infected sub- ... See full document

... construction. nef alleles predicting the five consensus Nef amino acid sequences were constructed using a combination of splice-overlap extension PCR mutagenesis and standard DNA ...consensus nef ... See full document

... a virus from PBMCs cocultured with PHA-stimulated normal donor ...HIV-1-related disease, including hepatosplenomegaly and generalized lymphadenopathy, and a precipitously fall- ...FIG. 1. ... See full document

... of human immunodeficiency virus type 1 (HIV-1) infection provide an oppor- tunity to investigate both viral and host factors that influence the rate of disease ...the ... See full document

... A nef gene is present in all primate lentiviruses, including human immunodeficiency virus type 1 (HIV-1) and simian immunodeficiency virus of macaque monkeys ... See full document

... the Nef-associated kinase identify it as a member of the PAK ...From sequence data there appear to be a minimum of three mammalian PAK members: (i) rat p65PAK or rat a PAK (36) and human PAK1 ... See full document

... be associated with disease ...HIV-1 disease progression may bias conclu- sions regarding virologic associations with disease ...of nef genes from patients representing the entire ... See full document

... Comparison of frequency of monocytotropic HIV-1 clones in peripheral blood bronchoalveolar lavage cellsPercentage CD4+ T 7~~~~~~100 Phenotype o' virus clones 0 Si T,MDM Origin of patient[r] ... See full document

... transporter associated with processing complex (TAP) and therefore are unable to transport antigens through the class I pathway (52) for presentation and recognition by ...five different CTL clones ... See full document

... DNA sequence analysis of ptrENV revealed the presence of a frameshift mutation at a BglII site in the nef gene due to the insertion of 4 ...LAI Nef protein, followed by 2 unrelated ...truncated ... See full document

... The presence of HIV DNA with NSI sequences in CCR5- negative cells was intriguing. This might suggest that our cur- rent phenotype prediction by V1V2 and V3 sequences is not accurate. Alternatively, the lymphocytes that ... See full document

... 10, which potentially affect the GTPase and GTP binding activities; iii different Nef aminoterminal sequence in the recombinant Nef protein, resulting from initiation at a putative start[r] ... See full document

... Nucleotide sequence variation in ...of nef in detail, a total of 90 full-length nef nucleotide sequences were obtained from 10 long-term survi- ...length variations, although intrasample ... See full document

... Human immunodeficiency virus type 1 (HIV-1) Nef is a multifunctional protein that confers an ability to evade killing by cytotoxic T lymphocytes (CTLs) as well as other ... See full document

... within Nef sequences from a single patient at a single time point to be of interest, (ii) we consider variation in Nef sequences from a single patient at different time points to be potentially ... See full document

... virion- associated tRNA and genomic ...D nef virions, indicating that these viruses had the same capacities to synthesize minus-strand viral DNA in ... See full document

... of Nef are impaired in their ability to reverse transcribe the viral RNA genome (5, 24, 68), indicating that Nef facilitates an early step of the viral replication ...of Nef becomes manifest at the ... See full document

... TSG101-depleted, Nef-expressing cells, CD4 accumulated within large vesicles that were often decorated by the ESCRT-0 component HRS ...from Nef- induced degradation, as shown by immunoblot analysis of total ... See full document

... of Nef binds tyrosine kinases of the Src family, including Lck, Hck, Vav, and serine-threonine kinases such as the Nef-associated kinase and PAK2 (32; for a review, see reference ...involving ... See full document

... pro- virus integrates next to the sequence that is transduced (3, ...chimeric virus/cellular mRNA into a virion or aberrant splicing yielding a chimeric mRNA which is then encapsidated into a virion ... See full document