[PDF] Top 20 Sequences Downstream of the 5′ Splice Donor Site Are Required for both Packaging and Dimerization of Human Immunodeficiency Virus Type 1 RNA
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Sequences Downstream of the 5′ Splice Donor Site Are Required for both Packaging and Dimerization of Human Immunodeficiency Virus Type 1 RNA
... of human immunodeficiency virus type 1 RNA are incorporated into each virus particle and are further converted to a stable dimer as the virus particle ...Several ... See full document
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Maintenance of the Gag/Gag-Pol Ratio Is Important for Human Immunodeficiency Virus Type 1 RNA Dimerization and Viral Infectivity
... virion RNA packaging. For virion RNA analysis, HxB2-BH10 proviral DNA was replaced with ⌬Rev proviral DNA in a ...the virus-producing cell, as both Gag (from the ⌬Rev plasmid) and Rev ... See full document
8
Viral RNA Is Required for the Association of APOBEC3G with Human Immunodeficiency Virus Type 1 Nucleoprotein Complexes
... lanes 1 and 2), APO3G packaging into mS.1 was re- duced by ⬎ 3-fold compared to ...the 5 ⬘ -untranslated region, they clearly identify SL1 as a region important for packaging of APO3G ... See full document
5
RNA Structure and Packaging Signals in the 5′ Leader Region of the Human Immunodeficiency Virus Type 1 Genome
... FIG. 1. Mutant HIV-1 RNAs characterized in this study. (A) The 5⬘ leader region of wild-type HIV-1 RNA from strain HXB2, with the gag initiation codon shown with open lettering ... See full document
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Mutations in the kissing-loop hairpin of human immunodeficiency virus type 1 reduce viral infectivity as well as genomic RNA packaging and dimerization.
... 59 splice junction, or RNA translation from the further downstream AUG initiation codon of the gag ...viral RNA, which itself requires near normal expression of Tat and Rev, which are ... See full document
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Dissociation of Genome Dimerization from Packaging Functions and Virion Maturation of Human Immunodeficiency Virus Type 1
... wild type (see ...its RNA, since the infectivities of other mutants that showed various RNA profiles also displayed the same magnitude of ...viral RNA could be the template for reverse ... See full document
9
Repair of a Rev-Minus Human Immunodeficiency Virus Type 1 Mutant by Activation of a Cryptic Splice Site
... viral RNA at 2 days posttransfection. Sequences encompassing the tat gene were amplified in a reverse tran- scription (RT)-PCR (primer positions are indicated in ...all virus variants ...exon ... See full document
6
RNA secondary structure and binding sites for gag gene products in the 5' packaging signal of human immunodeficiency virus type 1.
... cis-acting sequences at several locations in the HIV-1 genome may contribute to packaging (9, 30, 32, 33, 43), we elected to focus primarily on the 206-base region spanning nucleotides 632 to 837 of ... See full document
9
Gag-Processing Defect of Human Immunodeficiency Virus Type 1 Integrase E246 and G247 Mutants Is Caused by Activation of an Overlapping 5′ Splice Site
... wild type were present in pNLD2up-transfected cells ...FIG. 1. Viral RNA produced within HIV-1-infected cells and virus mutants used in this ...(ii) 5 ⬘ SS and 3 ⬘ SS located ... See full document
5
Nucleocapsid and Matrix Protein Contributions to Selective Human Immunodeficiency Virus Type 1 Genomic RNA Packaging
... of RNA incorporation in mutated viral ...genomic RNA incorporation but increased incorporation of spliced viral mRNA (7, 13, ...initiation site beyond the first splice donor site ... See full document
11
RNA Structure Modulates Splicing Efficiency at the Human Immunodeficiency Virus Type 1 Major Splice Donor
... The human immunodeficiency virus type 1 (HIV-1) proviral DNA genome expresses a primary transcript of 9 kb that serves not only as genomic RNA for progeny virus but ... See full document
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Dimerization and Template Switching in the 5′ Untranslated Region between Various Subtypes of Human Immunodeficiency Virus Type 1
... The human immunodeficiency virus type 1 (HIV-1) particle contains two identical RNA strands, each corresponding to the entire ...The 5 ⴕ untranslated region (UTR) ... See full document
11
Requirements for kissing-loop-mediated dimerization of human immunodeficiency virus RNA.
... viral RNA strands is proposed to occur initially at a conserved, six-base, GC-rich palindrome in the SL1 loop (13, 19, ...specifically required at the two central ...SIV sequences may simply reflect ... See full document
7
RNA Splicing at Human Immunodeficiency Virus Type 1 3′ Splice Site A2 Is Regulated by Binding of hnRNP A/B Proteins to an Exonic Splicing Silencer Element
... 3⬘ splice site A2, which defines the 5⬘ border of noncoding exon ...is 5⬘ splice site D3, which defines the 3⬘ boundary of the exon and is a weak splice ...silencer ... See full document
11
Possible Role of Dimerization in Human Immunodeficiency Virus Type 1 Genome RNA Packaging
... the packaging and dimerization functions ...for dimerization and not for packaging, whereas we observed re- gions functional for packaging but not dimerization ...for ... See full document
10
A dual role of the putative RNA dimerization initiation site of human immunodeficiency virus type 1 in genomic RNA packaging and proviral DNA synthesis.
... homologous RNA molecules that are tightly associated through numerous interactions (7, 38, ...of RNA extracted from virions revealed that these RNA molecules are joined noncovalently at a discrete ... See full document
7
An Exonic Splicing Silencer Downstream of the 3′ Splice Site A2 Is Required for Efficient Human Immunodeficiency Virus Type 1 Replication
... is required for efficient virus repli- ...and 5 ⬘ ss ...activity downstream of HIV-1 3 ⬘ ss A2 is localized to a 16-nt element within the vif coding ...binding site that was ... See full document
9
cis-acting sequences involved in human immunodeficiency virus type 1 RNA packaging.
... Retroviral RNA packaging is a specific process involving interactions among cis-acting sequences in the RNA genome and viral structural ...In human immunodeficiency virus ... See full document
5
Mechanisms of Human Immunodeficiency Virus Type 2 RNA Packaging: Efficient trans Packaging and Selection of RNA Copackaging Partners
... the RNA copack- aging in HIV-1, and we observed that ⬃ 45% of the viral particles are heterozygous or ⬃ 48% of the particles with RNA signals have two different RNAs, a frequency higher than that of ... See full document
10
Nonreciprocal Packaging of Human Immunodeficiency Virus Type 1 and Type 2 RNA: a Possible Role for the p2 Domain of Gag in RNA Encapsidation
... of human immunodeficiency virus types 1 (HIV-1) and 2 (HIV-2) to cross-package each other’s RNA was investigated by cotransfecting helper virus constructs with vectors ... See full document
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