[PDF] Top 20 Simultaneous Detection of Influenza Viruses A and B Using Real Time Quantitative PCR
Has 10000 "Simultaneous Detection of Influenza Viruses A and B Using Real Time Quantitative PCR" found on our website. Below are the top 20 most common "Simultaneous Detection of Influenza Viruses A and B Using Real Time Quantitative PCR".
Simultaneous Detection of Influenza Viruses A and B Using Real Time Quantitative PCR
... Virus isolation and growth. Confluent tertiary rhesus monkey kidney cells were inoculated with 100 l of each clinical sample. After absorption for 1 h at room temperature, the inoculum was removed and 5 ml of fresh ... See full document
5
Development and Validation of a One Step Real Time PCR Assay for Simultaneous Detection of Subtype H5, H7, and H9 Avian Influenza Viruses
... the detection of AI infection, with VI in eggs universally rec- ognized as the gold ...is time consuming and requires facilities ...a real-time hydrolysis probe-based RT-PCR assay for ... See full document
5
Development of a duplex TaqMan real time RT PCR assay for simultaneous detection of newly emerged H5N6 influenza viruses
... duplex real-time RT- PCR assay was developed to identify H5N6 subtype ...hands-on time. Contrasting to single probe rRT- PCR, this method introduced two different labeled probes so that ... See full document
7
Evaluation of a Multiplex Real Time Reverse Transcriptase PCR Assay for Detection and Differentiation of Influenza Viruses A and B during the 2001 2002 Influenza Season in Israel
... the time of receipt of the ...multiplex influenza virus TaqMan assay in Israel is about ...evaluated using a large number of clinical sam- ...the influenza virus B-specific probe, ... See full document
7
Real time reverse transcription PCR based sequencing independent pathotyping of Eurasian avian influenza A viruses of subtype H7
... In total, 75 samples positive for AIV of subtype H7 were used. Based on nucleotide sequence analysis and/or IVPI, 49 samples were classified as LPAIV and 15 as HPAIV (Table 4, Additional file 1: Figure S1). They were of ... See full document
10
Simultaneous detection of eight immunosuppressive chicken viruses using a GeXP analyser based multiplex PCR assay
... Specific PCR amplicons for each virus were individu- ally cloned into the pGEM-T vector (Promega, Madison, USA), and the plasmids were purified and ...ssRNA using the RiboMAXLarge Scale RNA Production Sys- ... See full document
12
Quantitative detection of human Malawi polyomavirus in nasopharyngeal aspirates, sera, and feces in Beijing, China, using real time TaqMan based PCR
... different detection methods used. The detection of MWPyV in the feces of children with diarrhea indicated that MWPyV may not be a strong can- didate for gastroenteritis in children because of the co- ... See full document
8
Simultaneous Detection, Subgrouping, and Quantitation of Respiratory Syncytial Virus A and B by Real Time PCR
... were influenza virus [A/New Caledonia/20/99 (H1N1), A/Sydney/5/97(H3N2), and ...94 viruses derived from 175 well-characterized clinical respiratory specimens, described previously, were ex- amined ...1; ... See full document
6
A triplex quantitative real time PCR assay for differential detection of human adenovirus serotypes 2, 3 and 7
... differential detection of HAdV2, 3, and 7 from other serotypes in China should be of great significance in clinical ...Species B HAdV types 3 and 7 have a high probability of disease ...C viruses ... See full document
6
Quantitative Detection of Borrelia burgdorferi by Real Time PCR
... by real-time PCR up to 7 months after infection (data not ...protein PCR applying phosphoimage analysis of radioactively labelled PCR products ... See full document
6
Real Time PCR for Quantitative Detection ofToxoplasma gondii
... Several PCR-based techniques have been developed as alternative diagnostic measurements for ...gondii detection originated with Burg et al. in 1989 (2), who combined PCR amplification with Southern ... See full document
5
Real Time Quantitative PCR Assays for Detection and Monitoring of Pathogenic Human Viruses in Immunosuppressed Pediatric Patients
... 23 real-time PCR assays based on TaqMan technology has been developed for the detection and monitoring of 16 different viruses and virus families including human polyomaviruses BK virus ... See full document
10
A sensitive one step real time PCR for detection of avian influenza viruses using a MGB probe and an internal positive control
... the detection limit of the assay as a pos- itive ...purified using the QIAquick gel extraction kit (Qiagen) and ligated to the ...vector using the TOPO™ TA cloning kit (Invitrogen Corp, Carlsbad, ... See full document
8
Simultaneous detection and differentiation by multiplex real time RT PCR of highly pathogenic avian influenza subtype H5N1 classic (clade 2 2 1 proper) and escape mutant (clade 2 2 1 variant) lineages in Egypt
... avian influenza virus (HPAIV) of subtype H5N1 in Egypt continues to devastate the local poultry industry and poses a permanent threat for human ...Egypt. Viruses of the ...escaped detection by ... See full document
8
Development of real time PCR for detection and quantitation of Dengue Viruses
... Three hundred and eight blood samples were used to eval- uate the usefulness of the qRT-PCR assay for its diagnostic potential. The blood samples were obtained within 10 days post onset of symptoms. The sera were ... See full document
8
Real Time Quantitative PCR for Detection of Helicobacter pylori
... The classical protocol for isolating nucleic acid from rela- tively large amounts of tissue (200 mg to 1 g) is the proteinase K-phenol method. For isolating nucleic acids from small amounts of tissue, there are no ... See full document
9
Hepatitis E Virus (HEV) Detection and Quantification by a Real Time Reverse Transcription PCR Assay Calibrated to the World Health Organization Standard for HEV RNA
... A real-time reverse transcription-quantitative PCR (RT-qPCR) assay utilizing Pleiades probe chemistry and an RNA internal control for the simultaneous detection and quantification ... See full document
10
Multiplex reverse transcription PCR for surveillance of influenza A and B viruses in England and Wales in 1995 and 1996
... for detection, typing, and subtyping of the hemagglutinin gene of influenza type A (H3N2 and H1N1) and type B viruses was developed and applied prospectively to virological surveillance of ... See full document
7
Impact of Early Detection of Respiratory Viruses by Multiplex PCR Assay on Clinical Outcomes in Adult Patients
... espiratory viruses can cause not only mild upper respiratory tract infections but severe pneumonia, especially in immuno- compromised ...for detection of viral pathogens are increasingly used due to their ... See full document
8
Design and Validation of an H5 TaqMan Real Time One Step Reverse Transcription PCR and Confirmatory Assays for Diagnosis and Verification of Influenza A Virus H5 Infections in Humans
... of influenza viruses belonging to all three H5 HA clades, three degenerate bases were incor- porated into the forward primer and one was incorporated into the probe ...The influenza A H5N3 virus ... See full document
9
Related subjects