[PDF] Top 20 Single cell sequencing: a distinct new field
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Single cell sequencing: a distinct new field
... sequence single TM7 bacterial cells from the soil and gained a deep insight into the evolution and metabolism of these cells ...the single cell genomic sequencing in other candidate uncultured ... See full document
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Understanding tumor ecosystems by single cell sequencing: promises and limitations
... five distinct types of tumor-resident fibroblasts were identified that expressed unique reper- toires of collagens and other extracellular matrix mole- ...two distinct subtypes of CAFs were identified, one ... See full document
14
Scalable microfluidics for single cell RNA printing and sequencing
... between cell-identifying ...during cell lysis and RNA capture, any imperfections in sealing and washing could lead to inter-well contamination as dem- onstrated in Figure S3 in Additional file ...both ... See full document
16
Single cell RNA sequencing of stem cell derived retinal ganglion cells
... RNA sequencing (RNA-seq) technology has allowed for the rapid quantification of individual gene ...the cell population being studied. Recent developments to isolate single cells, and barcode their ... See full document
9
A comparison of automatic cell identification methods for single cell RNA sequencing data
... assign cell identities including single-cell- specific and general-purpose ...available single-cell RNA se- quencing datasets of different sizes, technologies, species, and ...in ... See full document
19
Single Cell Genome and Group Specific dsrAB Sequencing Implicate Marine Members of the Class Dehalococcoidia (Phylum Chloroflexi) in Sulfur Cycling
... the distinct monophyletic clustering of the eight clone sequences with DsrA of DEH-C11 compared to other stable family-level lineages as shown by using all three approaches and on the fact that the best BLASTX ... See full document
15
Design and computational analysis of single cell RNA sequencing experiments
... cell’s distinct features may be due to interesting biological processes and not technical ...a cell that passes quality control individually but not in the group ...the single-cell ...of ... See full document
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Novel Methods in the Study of the Breast Cancer Genome: Towards a Better Understanding of the Disease of Breast Cancer
... heterogeneous cell populations in the spatial ...normal cell populations may contrib- ute to more than 50% of the total extracted DNA or RNA ...depth sequencing to obtain sufficient se- quenced data ... See full document
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Sensitivity to sequencing depth in single-cell cancer genomics
... Raw single-nucleotide variant (SNV) calls for the bulk datasets were obtained using the paired-sample variant- calling approach implemented in the VarDict software ...two distinct categories: “germline” ... See full document
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Sequencing of emerging canine distemper virus strain reveals new distinct genetic lineage in the United States associated with disease in wildlife and domestic canine populations
... Approximately 50 bp overlapping primer sets with ~1000 bp products were designed using Primer 3 [27, 28] to amplify complete CDV genome sequence (minus the extreme 5’ and 3’ non-coding ends) (Table 2). Two μL of RNA per ... See full document
10
Extraction of total RNA from single oocytes and single cell mRNA sequencing of swine oocytes
... of single oocytes are essential for a fine dissection of molecular features governing developmen- tal ...from single oocytes showed distinct peaks for 18S and 28S ribosomal ...from single ... See full document
5
Single-cell sequencing and tumorigenesis: improved understanding of tumor evolution and metastasis
... a single clonal expansion, but the other carcinoma was genetically heterogeneous, containing distinct clonal subpopulations of cells that were hypothesized to have originated early in tumor ...Further ... See full document
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Current Progresses of Single Cell DNA Sequencing in Breast Cancer Research
... first single cell DNA sequencing research in cancer was published by Navin ...100 single cells of a polygenomic triple negative (ER-/PR-/Her2-) breast cancer and 100 single cells of a ... See full document
12
High-resolution single-cell sequencing of malaria parasites.
... In the 48 h blood stage of the malaria lifecycle, parasites are haploid and in the earliest stage of their life cycle contain only a single copy of the genome. By the latest life cycle stage, they harbor 16 copies ... See full document
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The single-cell sequencing: new developments and medical applications
... gle-cell sequencing methods ...the single-cell level. Vitak et al. [3] proposed a single-cell combinatorial marker sequencing technique (SCI-seq) that can simultaneously ... See full document
9
SCOPE Seq: a scalable technology for linking live cell imaging and single cell RNA sequencing
... of single-cell RNA-Seq (scRNA-Seq) ...these new tools do not offer a straightforward way to directly link pheno- typic information obtained from individual, live cells to their expression ...live ... See full document
5
Single cell sequencing in stem cell biology
... [73]. New methods covering more layers of different omics are expected to emerge in the near ...individual cell, an ideal approach would be to use single-cell genome sequencing data to ... See full document
12
Single-Cell Epigenomics In Cancer Research
... bisulfite sequencing, both GpC sites (methylated in accessible chromatin regions by MTase) and endogenous CpG methylation sites can be simultaneously analysed, allowing profiling of chromatin accessibility and ... See full document
8
Single-cell RNA-sequencing of the brain
... CEL‑seq: cell expression by linear amplification and sequencing; CNS: central nervous system; COP: committed oligodendrocyte precursors; CPM: counts per million mapped reads; CV: coefficient of variation; ... See full document
14
Genotype free demultiplexing of pooled single cell RNA seq
... We used a single scRNA-seq BAM file from Zheng et al. [2] as a template for simulation. Additionally, we took 32 samples from genoptype information used in figure 2 supplementary data of Kang et al. [6], as the ... See full document
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