[PDF] Top 20 Spa 1 regulates the maintenance and differentiation of human embryonic stem cells

... ABSTRACT Human embryonic stem cells (hESCs) are pluripotent, whereby they can proliferate endlessly and differentiate into many different cell ...dynamics. Spa-1 is a principal ... See full document

... altered differentiation and adaptation. Cultures of hES cells are typically heterogeneous with mixtures of the undifferen- tiated stem cells and their differentiated ...hES cells form a ... See full document

... Total RNA was extracted using an RNeasy Mini Kit (Qiagen, Germany) and quantified using an ND1000 Spectrophotometer (NanoDrop Technologies, Inc., DE, USA). Then 500 ng was subjected to reverse transcrip- tion for cDNA ... See full document

... fragments are then placed into tissue and protected from direct trauma during wound healing. Resumption of mea- surable, normal parathyroid function occurs in 6–10 weeks for patients who otherwise have no endogenous ... See full document

... anti- differentiation cytokines such as BMP4 and LIF (Martin, 1981, Qi et ...ICM cells and is involved in the maintenance of pluripotency in the mouse embryo (Nichols et ...neural ... See full document

... feeder cells have been adopted for the culture of human embryonic stem cells (hESCs) to improve their attachment and provide them with stemness-supporting ...feeder cells differ ... See full document

... stem cells divide asymmetrically [71]. The cleavage planes of these cells are supposed to circumvent the cadherin hole to the daughter ...of embryonic distal lung epithelial ...daughter ... See full document

... ␣ regulates HCMV infection in hESC-derived NPC remains to be ...during differentiation, which could be further analyzed by global gene expression ...subsequent differentiation time points (Fig. ... See full document

... affecting human neural tube formation under controlled conditions (Gerrard et ...progenitor cells, in our system (stage ...neuronal differentiation in a reproducible way (Bain et ...neuronal ... See full document

... ES cells contribute to all cell types in the chimeric progeny mice, in- cluding the germ ...ES cells are born (3,4). (B) Subcutaneous injection of embry- onic stem cells into syngeneic mice ... See full document

... of human embryonic stem cells (hESCs) was first described by Sato and colleagues [20], identifying about 900 genes including PSMB8/β5i among the top ...the maintenance of ...pluripotent ... See full document

... in differentiation) to ~8% (relative to total EBs counted later in ...cardiac differentiation with batch selected serum (He et ...endoderm-like cells (Passier et ... See full document

... A549 cells (AEC) were purchased from ATCC, Rockville, USA and cultured in DMEM (Gibco, Invitrogen, USA) supplemented with 10% FBS (Lonza, Belgium), 1% L-glutamine (Lonza, Belgium) and 1% NEAA ... See full document

... element (TRE)-controlled Tcf7l1 transgene along with a tetracycline-controlled transactivator (tTA) transgene (Fig. 2a). In this model, supplemental doxycycline (dox) silences Tcf7l1 transgene expression, hence achieving ... See full document

... CA), 1% penicillin-streptomycin (Invitrogen), 10% fetal bovine serum (Sigma- Aldrich) and 1% l-glutamine ...hESCs, cells were grown in MEF-CM which lacked L- lysine and L-arginine, but contained 13 C ... See full document

... control human embryonic stem cell (hESC) line H9 (WiCell) [36] were maintained in the undifferentiated state using TeSR-E8 medium (Stem Cell ...H9 cells were not peculiar to the latter. ... See full document

... inductive differentiation of hESCs The hESC, ZZU-hESC-2, established by our cen- ter was used in this ...(KOSR), 1% non-essential amino acids (NEAA), 2mM L-glutamine, ... See full document

... mES cells via overexpression of six factors, Sry, Sox9, SF1, WT1, GATA4, and ...5 cells/cm 2 ...Sertoli cells from human em- bryonic stem cells by reducing the size of the ... See full document

... the human body with numerous mechanical and haematological ...mesenchymal stem cells has raised a major research ...mesenchymal stem cells (MSCs) in the osteogenic culture ...and ... See full document

... A3-1 cells using: sense (5’-TAA GAA TTC ACC ATG GCA TCC CAC ACG GCT GAT GC-3’) and antisense (5’-TTA CGC CGG CGT TAA GAC TCC ATC TGT GTT TCT CTT C-3’) oligonucleotide ... See full document