[PDF] Top 20 Specific proteolytic cleavage of recombinant Norwalk virus capsid protein.
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Specific proteolytic cleavage of recombinant Norwalk virus capsid protein.
... a specific cleavage of rNV cap- sid protein. Because specific proteolytic cleavage of a viral capsid protein is a common property in the life cycles of other ... See full document
6
Development of Norwalk Virus-Specific Monoclonal Antibodies with Therapeutic Potential for the Treatment of Norwalk Virus Gastroenteritis
... strain Norwalk virus (NV) (genogroup ...with Norwalk recombinant virus-like particles (rVLPs) were recovered, with estimated binding affinities in the subnanomolar ...a specific ... See full document
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Biochemical characterization of a smaller form of recombinant Norwalk virus capsids assembled in insect cells.
... NV capsid protein or by a cleaved or degraded fragment of this protein, 23- and 38-nm VLPs that were purified and stored in the presence of the protease in- hibitors aprotinin (Sigma, ...same ... See full document
7
Cleavage of the Feline Calicivirus Capsid Precursor Is Mediated by a Virus-Encoded Proteinase
... undergoes proteolytic processing during infection to produce the mature nonstructural ...structural protein that is translated as a 73-kDa precursor protein that is rapidly cleaved to yield the ... See full document
9
Specific in vitro cleavage of a Leishmania virus capsid-RNA-dependent RNA polymerase polyprotein by a host cysteine-like protease.
... Leishmania virus particles isolated from infected ...RNA virus 1-4 transcripts, we have developed an in vitro cleavage assay system to explore the possibility that the fusion polyprotein is ... See full document
8
Proteolytic cleavage analysis at the Murray Valley encephalitis virus NS1 2A junction
... NS1-2A cleavage was mostly abolished due to a Val to Phe substi- tution at the P3 residue in the octapeptide (see below), did not generate detectable NS2A, but NS1-2A precur- sor bands of increased intensity ... See full document
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Interaction of Recombinant Norwalk Virus Particles with the 105-Kilodalton Cellular Binding Protein, a Candidate Receptor Molecule for Virus Attachment
... Norwalk virus (NV), responsible for outbreaks of acute gastroenteritis, comprises the species of the genus Norwalk-like viruses in the family ...models, virus-like particles (VLPs) generated ... See full document
9
Proteolytic Cleavage of the Fusion Protein but Not Membrane Fusion Is Required for Measles Virus-Induced Immunosuppression In Vitro
... H protein, the cells were subse- quently stained using an H-specific antibody ...F protein with HLA-DR molecules were observed only to a minimal extent ...H protein cocapped with both the ... See full document
9
Leader of the Capsid Protein in Feline Calicivirus Promotes Replication of Norwalk Virus in Cell Culture
... Plasmid construction and generation of region-specific antisera. Standard recombinant DNA methods were used for the construction of plasmids. The con- sensus full-length clone of NV, pNV101, has been ... See full document
12
Expression and Self-Assembly of Norwalk Virus Capsid Protein from Venezuelan Equine Encephalitis Virus Replicons
... NLV capsid genes from VEE ...NV capsid gene inserted into a polycloning site (PCS) under control of the subgenomic 26S ...(VEE capsid and envelope glycoproteins) result in the packaging and release ... See full document
8
Proteolytic activity of hepatitis A virus 3C protein.
... Translation of T7 transcripts of these plasmids generates proteins containing 11 amino acids of vector sequences and 8 amino acids from the predicted C terminus of HAV protein 2C fused t[r] ... See full document
6
Identification of domains in rubella virus genomic RNA and capsid protein necessary for specific interaction.
... to capsid protein were constructed by using Bal 31 nuclease ...the capsid protein binding region in the viral ...Purified capsid protein (3 pmol per strip) immobilized on ... See full document
7
Norwalk Virus Minor Capsid Protein VP2 Associates within the VP1 Shell Domain
... Given that VP2 does not interact with VP1 at all of the icosa- hedrally equivalent positions, it is indeed challenging to deter- mine precisely how VP2 is oriented inside the capsid. Although several studies had ... See full document
8
Inhibition by zinc of rhinovirus protein cleavage: interaction of zinc with capsid polypeptides.
... Since at later times of infection a pool of capsid protein and viral RNA is present in infected cells 1, the rapid inhibition of virus formation by zinc suggested that zinc might be inte[r] ... See full document
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Maturation of giardiavirus capsid protein involves posttranslational proteolytic processing by a cysteine protease.
... 100-kDa protein was predominantly labelled after the 15-min pulse ...This protein could be immunoprecipitated by a -NT, suggesting that the sequence encoding peptide NT, lo- cated at the very beginning of ... See full document
6
Proteolytic cleavage of the E2 glycoprotein of murine coronavirus: host-dependent differences in proteolytic cleavage and cell fusion.
... Thus, host-dependent differences in the precise location of the cleavage site of E2, the rate of transport of cleaved E2 to the cell membrane, or the response of the cell membranes to th[r] ... See full document
9
Production of a Recombinant Major Inner Capsid Protein for Serological Detection of Epizootic Hemorrhagic Disease Virus
... VP7 protein from SF21 cells infected with recombinant ...crude recombinant virus Ac-Bac-EHDV1-VP7-N-His-infected cell lysate (lane 1) with an Ni-NTA purified VP7-1 N-His preparation (lane ... See full document
6
Recombinant expression of beak and feather disease virus capsid protein and assembly of virus like particles in Nicotiana benthamiana
... Recombinant BFDV CP expressed in insect cell culture spontaneously assembles into particles ranging in size from 16 to 22 nm in diameter, which falls within the size range reported for infectious virions (10–22 nm ... See full document
12
Mutations affecting cleavage at the p10 capsid protease cleavage site block Rous sarcoma virus replication
... were harvested three days post transfection and viewed at a magnification of 11,000× (Fig. 3, left) and 37,000× (Fig. 3, right). We were only able to examine wild type, M239F and M239G particles by EM, as we were unable ... See full document
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Expression, self-assembly, and antigenicity of the Norwalk virus capsid protein.
... 6527 Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest Norwalk virus capsid protein was produced by expression of the second and third open reading frames of the Norwalk v[r] ... See full document
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