[PDF] Top 20 Stem cells living with a Notch

... between Notch and Wnt signaling in the intestine under physiological conditions is not clear, but Notch signaling also plays an important role in the maintenance of ISCs as well as their commitment towards ... See full document

... of Notch pathway activation, we asked if the expression of NOTCH1 is essential for survival, migration and stem-like feature of HNSCC cells in ...of Notch pathway transcript expression by ... See full document

... in stem cells, we sought to investi- gate its ...HEK293T cells and then treated the cells with trichostatin A (TSA), an inhibitor of histone deacety- lases (HDACs) I and II and nicotinamide ... See full document

... progenitor cells generated by DSL-treated mammospheres was increased approximately 13-fold compared with cells from mammos- pheres cultured in the absence of DSL ...progenitor cells generated by ... See full document

... Whereas NOTCH signaling is dispensable for the earlier yolk sac–derived primitive and erythromyeloid phases of hematopoie- sis, knockout and chimeric analysis in murine embryos demon- strated that NOTCH1 signaling ... See full document

... We generated mouse BEND6 constructs in pCAG-IRES-GFP or pCAG- IRES-DsRed (Matsuda and Cepko, 2004) and sh-mBEND6 constructs in pll3.7-IRES-DsRed (Rubinson et al., 2003). Previously described plasmids included ... See full document

... Gliomas are defined as brain tumors of glial origin. Based on histology, gliomas have been classified into astrocytoma (70%), oligodendroglioma (10% to 30%), mixed oligoastrocytoma and ependymoma (<10%). Low- grade ... See full document

... germ cells entering meiosis decreased and the rate of meiotic prophase progression ...that stem cell number declined and suggesting there was an age-related downregulation of GLP-1/Notch ...germline ... See full document

... the cells were cultured near to 80% confluency, they were switched to serum free medium, after 24 h, the medium was har- vested and centrifuged at 3000 rpm for 20 ...OS cells was direct loaded onto the ... See full document

... follicle stem cells lacking Notch signaling contribute to epidermal homeostasis under normal ...follicle stem cells during the second telogen (~P50) and to determine their fate 35 days ... See full document

... Müller cells was performed as previously described ...Müller cells attached to the bottom of the flask. The cells were trypsinized and cultured in DMEM containing 20% FBS for six days to further ... See full document

... To overexpress DLL4, the full-length of DLL4 sequence was synthesized with the sequence released in Genebank (Accession number: NM_019074.2) as a template and inserted to the BamHI and XhoI sites of empty pcDNA3.1 vector ... See full document

... mesenchymal stem cells (BMSCs). This study aims to explore the role of Notch signaling pathway in mechanical stimulation of infrasound altering BMSCs ...inhibit Notch signaling pathway or ... See full document

... on Notch signaling and germ cell differentiation [9–13], the roles of these signal- ing molecules in vivo germ cell differentiation have not been ...of Notch signal- ing in the in vitro differentiation of ... See full document

... experiments, cells in 96-well plates were treated with phosphate-buffered saline (PBS) or recombinant rat JAG1 (R&D Systems) diluted in PBS to a final concentration of 10 μ g/ml and incubated overnight at ... See full document

... Figure 1. MiR-1275 is overexpressed in LUAD and correlates with tumor progression and poor survival in LUAD patients. (A) Heat map showing 8 top upregulated miRNAs in the microarray (fold change ≥2, P ＜ 0.05). (B) ... See full document

... Expression profile data were generated using limb skeletal muscle stem/progenitor cells from transgenic Tg:Pax7-nGFP mice (R.S. and S.T., unpublished). The full dataset will be published elsewhere, but is ... See full document

... Abstract: Recently, the human gene NOTCH1 has been found to be implicated in cancer cell metastasis and the maintenance of cancer stem cells. However, for breast cancer in particular, an association between ... See full document

... enhanced stem phenotype and resistance to PTX in breast cancer cells induced by HIF-2α is dependent on Notch pathway activation, we also tested changes in Notch pathway-related proteins in ... See full document

... basal stem/progenitor cell subpopulation after ...High cells [43]. Furthermore, Notch signaling was observed to reduce in vitro and in vivo proliferation of CD29 High cells while pro- moting ... See full document