[PDF] Top 20 Structural and functional characterization of human immunodeficiency virus tat protein.
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Structural and functional characterization of human immunodeficiency virus tat protein.
... The diminished ability of the mutant proteins with substitution of the basic residues to transactivate gene expression and the lack of detectable protein in the nucleus suggested the pre[r] ... See full document
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Structural and Functional Characterization of the Mumps Virus Phosphoprotein
... P protein, a single vector was constructed to express the MuV P protein in ...P protein was further purified by size-exclusion chromatog- raphy and analyzed by ...P protein was shown to be ... See full document
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Structural and Functional Characterization of Membrane Fusion Inhibitors with Extremely Potent Activity against Human Immunodeficiency Virus Type 1 (HIV-1), HIV-2, and Simian Immunodeficiency Virus
... and human serum albumin at a dose-dependent manner, and it exhibited extremely low cytotoxicity and a high genetic barrier to ...with human serum ...with human serum albumin (HSA) precoated in the ... See full document
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Functional Characterization of the Human Immunodeficiency Virus Type 1 Nef Acidic Domain
... the structural fea- tures of Nef required for MHC-I downregulation will be neces- sary before a complete mechanism can be ...Nef protein (Protein Data Bank entry ... See full document
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Structural and functional analysis of the human immunodeficiency virus type 2 Rev protein.
... Structural and functional characterization of HIV-2 Rev protein mutants Plasmid Mutationa pSV2neo BLpSVHIV-2Rev BLpSVA35-37 BLpSVA39-43 BLpSVA43-49 BLpSVA46-49 BLpSVA61-64 BLpSVA73-74 BL[r] ... See full document
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Functional Characterization of the Human Immunodeficiency Virus Type 1 Genome by Genetic Footprinting
... N-terminal  hairpin (positions 1208 to 1228) and the cyclo- philin A-binding region (1443 to 1472) of capsid were able to form viral particles but were defective in a step in early infec- tion (Fig. 11 and 12). X-ray ... See full document
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Purification and structural characterization of the putative gag-pol protease of human immunodeficiency virus.
... coli-expressed plO protein for amino acid sequencing, the bacterial extracts labeled with [35S]Met and [3H]Leu or [3H]Val were fractionated by RP HPLC and individual fractions were immun[r] ... See full document
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Inhibition of human immunodeficiency virus type 1 and type 2 Tat function by transdominant Tat protein localized to both the nucleus and cytoplasm.
... HIV-1 Tat protein and its functional domains (19, 28, 41, 57, 58) are shown in ...of Tat. To evaluate the ability of the mutant Tat proteins to activate HIV gene expression and to ... See full document
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Modulation of Sp1 Phosphorylation by Human Immunodeficiency Virus Type 1 Tat
... inactive Tat mutants that compete with wild-type Tat for Sp1 contact and ones that repress HIV-1 replication in cultured T cells ...wild-type Tat-Sp1 contact is physiologically important for pro- ... See full document
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Activation of transcription factor NF-kappaB by the Tat protein of human immunodeficiency virus type 1.
... 2. Protein-DNA interactions at the HIV-1 LTR in HL3T1 cells before and after Tat lipofection, analyzed by in vivo DMS and DNase I ...relevant protein binding sites in the 39 ...three ... See full document
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Potentiation of human immunodeficiency virus type 1 Tat by human cellular proteins.
... with Tat leads to increased ...HIV-1 Tat protein in cell extracts from HeLa cells is dependent on functional Tat and on the TAR element (14, 16) and is due to the increased processivity ... See full document
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Functional Surfaces of the Human Immunodeficiency Virus Type 1 Capsid Protein
... the structural Gag polyprotein or its proteolytic products (see references 31, 41, 54, 79, 85, 90 and 97 for ...unprocessed human im- munodeficiency virus type 1 (HIV-1) Gag molecules assemble ... See full document
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Molecular cloning and characterization of a cellular protein that interacts with the human immunodeficiency virus type 1 Tat transactivator and encodes a strong transcriptional activation domain.
... simian virus 40 nuclear localization signal (PKKKRKV) at the N terminus, to generate pCMV-nucl-ATG-TAP208–260; the correct orientation was established by PCR analysis with primers 18 (upstream) and 8 ...of ... See full document
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Interaction of Human Immunodeficiency Virus Type 1 Tat with the Transcriptional Coactivators p300 and CREB Binding Protein
... The human immunodeficiency virus type 1 (HIV-1) transac- tivator of transcription (Tat) stimulates viral gene expression through an RNA element in the viral long terminal repeat (LTR) (14, ... See full document
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Functional Domains of Tat Required for Efficient Human Immunodeficiency Virus Type 1 Reverse Transcription
... lack tat can be complemented by the expression of a functional tat gene in the cell lines producing the mutant ...HIV-1 Tat is required at early stages of reverse transcription, al- though its ... See full document
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Synthesis of functional human immunodeficiency virus tat protein in baculovirus as determined by a cell-cell fusion assay.
... Abundant synthesis of functional human T-cell leukemia virus type I p4Y protein in eucaryotic cells by using a baculovirus expression vector.. A baculovirus vector can express intron-con[r] ... See full document
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Human immunodeficiency virus Tat induces functional unresponsiveness in T cells.
... with human immunodefi- ciency virus type 1 (HIV-1) is a remarkable loss of cell- mediated ...A functional abnormality of both CD4 1 and CD8 1 T cells in vivo in the early stages of HIV-1 infection ... See full document
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Functional Differences between Human and Bovine Immunodeficiency Virus Tat Transcription Factors
... bTat protein binds CycT1 ...fusion protein that can be readily detected using an anti-Rev ...fusion protein can potently activate an HIV-1 LTR in which the hTAR element has been replaced with Rev ... See full document
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Molecular determinants for cellular uptake of Tat protein of human immunodeficiency virus type 1 in brain cells.
... exogenous Tat to be taken up by cells has generated considerable interest due to the potential biotech- nological applications whereby Tat can be used as a vehicle for delivering heterogeneous proteins and ... See full document
5
Structural analysis of wild-type and mutant human immunodeficiency virus type 1 Tat proteins.
... These mutants, wild type in structure but reduced in activity, identify residues in the wild-type protein that may directly contact other molecules during Tat function... STRUCTURE OF HI[r] ... See full document
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