[PDF] Top 20 Structural and Functional Characterization of an Influenza Virus RNA Polymerase-Genomic RNA Complex
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Structural and Functional Characterization of an Influenza Virus RNA Polymerase-Genomic RNA Complex
... eIF4F complex, and the eluted material was subjected to Ni 2⫹ -NTA agarose chro- ...three polymerase subunits, as documented by Western blotting with ...the polymerase heterotrimer. (A) Various ... See full document
11
Structural and Functional Characterization of Sapovirus RNA-Dependent RNA Polymerase
... initiate RNA synthesis on homopolymeric poly(C) templates in the presence of high concentrations of GTP ...important structural and functional properties that separate calicivirus ... See full document
14
Structural and functional characterization of Newcastle disease virus polycistronic RNA species.
... With the exception of the 1.57 x 106-dalton species, all the 22S-28S RNAs, as well as the 3.02 x 106-dalton species, were reduced slightly in size after this protocol, consistent with th[r] ... See full document
6
Interaction of the Influenza A Virus Nucleocapsid Protein with the Viral RNA Polymerase Potentiates Unprimed Viral RNA Replication
... viral polymerase potentiates unprimed viral RNA replication has not been de- ...the polymerase proteins leads to a confor- mational change in the polymerase complex that activates ... See full document
8
Involvement of Influenza Virus PA Subunit in Assembly of Functional RNA Polymerase Complexes
... three polymerase proteins, including PA, appear in the nucleus in late phases of infection (1) ...a complex between PB1 and PA ...of polymerase functions through the conformational change of ... See full document
Characterization of the RNA-fork model of virion RNA in the initiation of transcription in influenza A virus.
... endogenous RNA allowed us to carry out this detailed study of the function of the 5 9 end in the initiation of transcription, we cannot exclude the possibility that this additional mutation might have affected our ... See full document
8
Expression of Functional Influenza Virus RNA Polymerase in the Methylotrophic Yeast Pichia pastoris
... When RNA synthesis was carried out with the plus-strand c53 template, the 3P complex produced two products ...major RNA migrated slightly faster than c53. The synthesis of both RNA products ... See full document
11
Comprehensive Proteomic Analysis of Influenza Virus Polymerase Complex Reveals a Novel Association with Mitochondrial Proteins and RNA Polymerase Accessory Factors
... shared RNA-independent PA- binding partners since these proteins were present in all three purified sample preparations ...for influenza virus endocytosis ...the influenza virus ... See full document
13
Diversity of the RNA Polymerase in the H7N9 Influenza A Virus
... and structural analyses recog- nize PB1 as responsible for RNA chain elongation ...all RNA- dependent RNA polymerases and RNA-dependent DNA polymerases ...the complex inactive ... See full document
7
Association of the Influenza A Virus RNA-Dependent RNA Polymerase with Cellular RNA Polymerase II
... and influenza virus ...trimeric influenza virus polymerase complex or with the indi- vidual polymerase ...expressed influenza virus polymerase ... See full document
7
Identification and Characterization of the 480-Kilodalton Template-Specific RNA-Dependent RNA Polymerase Complex of Red Clover Necrotic Mosaic Virus
... RCNMV genomic RNAs, they should be much larger than 480 kDa, because the pre- dicted molecular masses of RNA1 and RNA2 are 1,340 kDa and 500 kDa, ...which genomic RNAs were released during electrophoresis ... See full document
12
Role of Ran Binding Protein 5 in Nuclear Import and Assembly of the Influenza Virus RNA Polymerase Complex
... A virus NP, the major component of vRNPs, is known to interact with several members of the importin ␣ family of proteins, and it was proposed that these import re- ceptors play a role in the nuclear import of ... See full document
9
The influenza A virus PB2 polymerase subunit is required for the replication of viral RNA.
... the complex may alter the confor- mation of either PB1 or PA proteins or may affect their inter- ...the polymerase interact to form a trimeric structure (11, 19, ...the complex have been studied by ... See full document
5
Differential activation of the influenza virus polymerase via template RNA binding.
... Template RNA containing only the 3 9 end did not efficiently promote mRNA binding by the ...the polymerase for isolated 3 9 end (25). However, template RNA containing only the 5 9 end of vRNA was ... See full document
5
The PB2 Subunit of the Influenza A Virus RNA Polymerase Is Imported into the Mitochondrial Matrix
... for influenza A virus protein PB1-F2, which is also targeted to the mitochondria and inhibits MAVS-mediated inter- feron expression by decreasing MMP ...regulate virus induced interferon expression ... See full document
10
Isolation and Properties of an RNA Polymerase from Influenza Virus-Infected Cells
... A, to lyse membranes prior to centrifugation in A portion offraction 4 from influenza virus-infected the discontinuous sucrose gradient, the yield BHK21-F cells, labeled from to 4 h with[r] ... See full document
8
Mechanism for inhibition of influenza virus RNA polymerase activity by matrix protein.
... added RNA template was suitable for the study of the mechanism of the anti-RNA synthesis activity of M1 ...The RNA synthesis system depending on the endogenous tem- plate in RNP complexes is thought ... See full document
7
The RNA Polymerase of Influenza Virus, Bound to the 5′ End of Virion RNA, Acts in cis To Polyadenylate mRNA
... incorporation assay (Fig. 3, lane 17). The 29 (G3C) mutant (717 nt long) was also synthesized and tested in the RT-PCR assay, in which it also failed to produce polyadenylated RNA under conditions in which cRNA ... See full document
6
VP3, a Structural Protein of Infectious Pancreatic Necrosis Virus, Interacts with RNA-Dependent RNA Polymerase VP1 and with Double-Stranded RNA
... Construction of expression vectors for two-hybrid, co-IP, and pull-down anal- yses. All PCR primers, cloning vectors, and resulting plasmid constructs are listed in Table 1. The primers were designed for directional ... See full document
12
Biochemical characterization of the fidelity of poliovirus RNA dependent RNA polymerase
... 20% gels were visualized on a Bio-Rad Personal Molecular Imager FX Phospho-Image analyzer and quantified using Quan-One software. Gel background was subtracted from each datapoint. Product yield in nM was determined by ... See full document
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