[PDF] Top 20 Structural insights into the function of type VI secretion system TssA subunits.
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Structural insights into the function of type VI secretion system TssA subunits.
... The type VI secretion system (T6SS) is a multi-protein complex that injects bacterial effector proteins into target ...present structural and biochemical studies on TssA ... See full document
17
Double Tubular Contractile Structure of the Type VI Secretion System Displays Striking Flexibility and Elasticity
... wall presumably has a direct impact on the cellular organelles that are anchored by transenvelope complexes, including many responsible for translocating virulence fac- tors. Here, we show that removal of cell wall ... See full document
11
Structural Insight into How Bacteria Prevent Interference between Multiple Divergent Type IV Secretion Systems
... With their strict conservation across the derived rickettsial families and strikingly higher rate of evolution than Rvh-I pro- teins (Fig. 2), it is likely that the functions of Rvh-II proteins are linked. It is also ... See full document
17
Structural Basis of Type 2 Secretion System Engagement between the Inner and Outer Bacterial Membranes
... promoting secretion of substrate proteins (toxins, effectors, and hydrolytic enzymes) across the outer ...These structural breakthroughs highlighted a perplexing problem, given that the secretins interact ... See full document
6
Unraveling the Self Assembly of the Pseudomonas aeruginosa XcpQ Secretin Periplasmic Domain Provides New Molecular Insights into Type II Secretion System Secreton Architecture and Dynamics
... Further experiments performed under reducing conditions revealed that secretin func- tionality is fully recovered upon reduction of the artificial disulfide bridge. This locking- mediated loss of activity indicates that ... See full document
17
Molecular and Structural Analysis of the Helicobacter pylori cag Type IV Secretion System Core Complex
... Bacterial type IV secretion systems (T4SSs) can function to export or import DNA, and can deliver effector proteins into a wide range of target ...the structural organization of T4SSs that ... See full document
10
Understanding the sequential activation of Type III and Type VI Secretion Systems in Salmonella typhimurium using Boolean modeling
... A structural comparison of Vibrio FlrC and Salmonella YfhA (having 62% sequence similarity) was performed to check the extent of their structural homology, which would imply a similar ... See full document
12
Physiological Heterogeneity Triggers Sibling Conflict Mediated by the Type VI Secretion System in an Aggregative Multicellular Bacterium
... a structural component of the delivery tube and the other acts as a specific adaptor for TsxE ...a structural component, as its gene is part of the core T6SS gene cluster, whereas vgrG2 is adjacent to ... See full document
15
Structural organisation of the type IV secretion systems
... which type IV secretion (T4S) systems form the most functionally ver- satile class ...of secretion systems from the ...T4S system to inject virulence proteins into mammalian host cells [8–10] ... See full document
9
Genetic Dissection of the Type VI Secretion System in Acinetobacter and Identification of a Novel Peptidoglycan Hydrolase, TagX, Required for Its Biogenesis
... apparatus function (32, 33), including a membrane complex and other essential structural elements (20, ...secretory system passes through the PG layer of the T6SS-producing ... See full document
17
The Tip of the VgrG Spike Is Essential to Functional Type VI Secretion System Assembly in Acinetobacter baumannii
... The type VI secretion system (T6SS) is a critical weapon in bacterial war- fare between Gram-negative ...sential structural component of all T6SSs reported to ...unknown ... See full document
14
Identification of Mammalian Proteins That Collaborate with Type III Secretion System Function: Involvement of a Chemokine Receptor in Supporting Translocon Activity
... Flow cytometric assay for YopE GAP activity using a FRET read- out. We previously developed fluorescence resonance energy transfer (FRET) assays that allow image analyses of Y. pseudotu- berculosis YopE GTPase-activating ... See full document
13
Structural and biochemical insights into the function and evolution of sulfoquinovosidases
... the function and properties of SQases, we mapped their physical locations onto the X-ray structure of the two SQases with PNPSQ bound and used in silico methods to evaluate the structural e ff ects of their ... See full document
9
Structural and biochemical insights into the function and evolution of sulfoquinovosidases
... the function and properties of SQases, we mapped their physical locations onto the X-ray structure of the two SQases with PNPSQ bound and used in silico methods to evaluate the structural effects of their ... See full document
22
A Novel Mechanism for Protein Delivery by the Type 3 Secretion System for Extracellularly Secreted Proteins
... ABSTRACT The type 3 secretion system (T3SS) is essential for bacterial virulence through delivering effector proteins directly into the host cytosol. Here, we identi- fied an alternative delivery ... See full document
19
Negative Autogenous Control of the Master Type III Secretion System Regulator HrpL in Pseudomonas syringae
... The type III secretion system (T3SS) is a principal virulence determi- nant of the model bacterial plant pathogen Pseudomonas ...extracytoplasmic function sigma factor HrpL coordinates the ... See full document
16
Postreplication Roles of the Brucella VirB Type IV Secretion System Uncovered via Conditional Expression of the VirB11 ATPase
... was either induced with ATc (100 ng/ml) for 4 h prior to infection or induced at the time of infection either for the duration of the experiment or for 6 h only for HA-VirB11 stability experiments. An MOI of 500 was used ... See full document
10
Peptidomimetic Small Molecules Disrupt Type IV Secretion System Activity in Diverse Bacterial Pathogens
... inhibit secretion of IL-8 and activa- tion of NF-B signaling in ...of type 1 pili or curli in ...IL-8 secretion and NF-B ac- tivation (C10 and KSK85 [Table 1]) in a dose-dependent manner ...IL-8 ... See full document
10
Extracytoplasmic stress responses induced by a model secretin : a dissertation presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Biochemistry at Massey University, Manawatū, New Zealand
... for secretion of effectors through type 2 and 3 secretion systems in ...the secretion system alone (sans secreted effector proteins) or by the secretins alone (without the accompanying ... See full document
244
Self Labeling Enzyme Tags for Analyses of Translocation of Type III Secretion System Effector Proteins
... A series of enzymes is compatible with translocation if fused to effector proteins, and enzymes such as  -lactamase, adenylate cyclase, Cre-Lox, and luciferases have been used to quantify translocation of effectors into ... See full document
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