[PDF] Top 20 Structure and Assembly of Intracellular Mature Vaccinia Virus: Isolated-Particle Analysis

... (left particle) and bottom (right particle) aspects of the ...IMV particle that is evidently eviscerating viral tubules (arrow) that are seen outside the particle, leaving a cavity (arrowhead) ... See full document

... during assembly, is functionally and structurally continuous with smooth-ER membranes; the latter are themselves continuous with the in- termediate compartment (IC) between the ER and the Golgi complex (6, 18, 31, ... See full document

... 28), vaccinia virus has been the most intensively studied member of the ...Poxviridae. Vaccinia virus is the focus of renewed interest arising from the possibil- ity of deliberate smallpox ... See full document

... Vaccinia virus (VV) undergoes a proteolytic processing to evolve from immature virus particles into intracellular mature virus ...a mature virus ...in mature ... See full document

... in assembly, consistent with the findings of earlier studies that used tunicamycin (69) and monensin (53) to inhibit glycosylation in infected ...(40) isolated a mutant of the porcine TGEV in which the sole ... See full document

... the mature core, the VP3 shell is fundamental to the structure of the particle and dictates the correct assembly of BTV ...atomic structure, while the complete structure of the B ... See full document

... viral assembly factories as well as in much smaller dispersed cytoplasmic puncta representing individual virus cores ...cell-associated virus), there were noticeably fewer puncta of B5 staining ... See full document

... the mature-virus nucleocapsid ...the isolated nucleocapsid ...the isolated nucleocapsid relative to the mature-virus nucleocapsid and the redistribution of density that occurs ... See full document

... the virus behaved similarly to a VV strain that grows permissively in these ...thorough analysis of its assembly in cultured human ...less virus to obtain a similar number of infected cells ... See full document

... recombinant virus designed to regulate the expression of the A5L gene was constructed in two steps, starting with the parent virus (vT7lacOI), which contains the lacI and T7 RNA polymerase ...resulting ... See full document

... EM analysis of negatively stained isolated HIV-1 cores re- vealed that they were not collapsed but exhibited a defined three-dimensional ...detailed analysis of their structure will require ... See full document

... SDS-PAGE and Western blot analyses of EV and IMV viri- ons. EEV and IMV particles were purified on CsCl gradients in which the two particles sediment differentially because of the extra membrane possessed by EV. ... See full document

... Sizes of IMV particles and core envelope in negatively stained or vitrified preparations Virus particle Length nm Width IMV, negative stained IMV, vitreous Core membrane, vitreous 363 ± [r] ... See full document

... our analysis with p32, which is present in purified IMV and for which a transmembrane domain has been postulated on the basis of its predicted amino acid sequence ...of vaccinia virus-infected cells, ... See full document

... of vaccinia virus mRNA capping enzyme is an autonomous bifunctional domain with triphosphatase and guanylyltransferase ...fibroma virus, molluscum contagiosum virus, and African swine fever ... See full document

... variola virus in humans (9). This close host-virus relationship is particularly important for evaluating the role of the complement system, given the species specificity of many complement proteins, ... See full document

... WR virus infections do not induce substantial expression of immunomodulatory genes in various cell lines (44, 63, 64), primary fibroblasts (65), and human/mouse monocyte cells (37, 40, ...data analysis ... See full document

... EM analysis were conducted in derivative human HT1080 cells that expressed Gag/Gag-Pol alone (HT-Fly ...of intracellular VLPs displaying similar Gag-labeling ...MVB. Analysis of several cell sections ... See full document

... Infectious intracellular mature vaccinia virus virions (IMV) form in juxtanuclear factory regions of the cytoplasm (5, 19) and are wrapped by a double membrane derived from trans-Golgi or ... See full document

... successfully isolated (designated vA6L-mut1 or ...ORF virus (ORFV) (Parapoxvirus), fowlpox virus (FWPV) (Avipoxvirus), sheeppox virus (SPPV) (Capripoxvirus), Shope fibroma virus (SFV) ... See full document