[PDF] Top 20 Structure-Based Mutational Analysis of the Hepatitis C Virus NS3 Helicase
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Structure-Based Mutational Analysis of the Hepatitis C Virus NS3 Helicase
... RNA helicase activity and the ...wild-type helicase) still exhibited sufficiently high RNA helicase activity (for example, H293A and ...previous mutational studies (21, ...The structure ... See full document
9
NS3 from Hepatitis C Virus Strain JFH-1 Is an Unusually Robust Helicase That Is Primed To Bind and Unwind Viral RNA
... ABSTRACT Hepatitis C viruses (HCV) encode a helicase enzyme that is essential for viral replication and assembly (nonstructural protein 3 ...This helicase has be- come the focus of extensive ... See full document
16
The NS3 Helicase and NS5B-to-3′X Regions Are Important for Efficient Hepatitis C Virus Strain JFH-1 Replication in Huh7 Cells
... Core protein and infectious-chimeric-virus secretion from chimeric J6CF RNA-transfected cells. Finally, we tested whether chimeric RNA-transfected cells could secrete infec- tious virus particles. Figure 5C ... See full document
11
Specific Interaction of Hepatitis C Virus Protease/Helicase NS3 with the 3′-Terminal Sequences of Viral Positive- and Negative-Strand RNA
... Hepatitis C virus (HCV) is the primary causative agent of parenterally transmitted non-A, non-B hepatitis and affects a significant part of the world’s ...chronic hepatitis, cirrhosis ... See full document
14
Stimulation of Hepatitis C Virus (HCV) Nonstructural Protein 3 (NS3) Helicase Activity by the NS3 Protease Domain and by HCV RNA-Dependent RNA Polymerase
... the NS3 protease, HCV RdRp (NS5B) was found to strikingly stimulate the NS3 helicase activity by a specific protein-protein interaction with the NS3 protease do- main ...NS3FL helicase ... See full document
11
Mutations That Affect Dimer Formation and Helicase Activity of the Hepatitis C Virus Helicase
... the helicase to assay dimerization and helicase ...in helicase activity of the three mu- tants is not due to their inability to bind the substrate, since all three mutants shifted the substrate to a ... See full document
10
Protease Inhibitors Block Multiple Functions of the NS3/4A Protease-Helicase during the Hepatitis C Virus Life Cycle
... of NS3 has been the focus of drug development efforts, NS3 is a bifunctional enzyme with sep- arate protease and helicase domains connected by a flexible ...The helicase domain has NTPase and ... See full document
9
Structurally Conserved Amino Acid W501 Is Required for RNA Helicase Activity but Is Not Essential for DNA Helicase Activity of Hepatitis C Virus NS3 Protein
... the helicase sub- strate ...the NS3 and the NS5B proteins. This speculation was based on the fact that HCV helicase strongly prefers poly(U) for the stim- ulation of ATPase activity (8, 34, ... See full document
12
Intracytoplasmic stable expression of IgG1 antibody targeting NS3 helicase inhibits replication of highly efficient hepatitis C Virus 2a clone
... A replication defective adenovirus construct carrying the gene for the recombinant human antibody (Ad-IgG1) was prepared using standard PCR and cloning methods. We used the pFab-CMV-NS3 (L+H) plasmid construct to ... See full document
12
Isolation and Characterization of Monoclonal Antibodies That Inhibit Hepatitis C Virus NS3 Protease
... investigating NS3 protease inhibitors, such as substrate or product mimetics (27, 37), high-throughput screening of chem- ical compounds (19), and a selection of affinity ligands from a large pool of ... See full document
9
Hepatitis C Virus Core Protein Interacts with Cellular Putative RNA Helicase
... and C), suggesting that FLAG z CAP-Rf, presumably CAP-Rf, could interact with both full-length/ma- tured and truncated forms of HCV core protein in cells, a result consistent with colocalization study ...and ... See full document
13
X-Ray Structure of the Pestivirus NS3 Helicase and Its Conformation in Solution
... and NS3 have enzymatic activities. NS3 has a molecular mass of about 76 kDa and features two main functional domains, each with different enzymatic ...The C-terminal do- main, about two-thirds of ... See full document
16
Generation and Characterization of a Hepatitis C Virus NS3 Protease-Dependent Bovine Viral Diarrhea Virus
... HCV NS3 protease ...addressed. Based on a prediction that the HCV NS3 protease might be deleted to yield a virus without Npro, we created a molecular clone lack- ing Npro (BVDV⫺Npro) in which ... See full document
9
Over-expression and characterization of NS3 and NS5A of Hepatitis C virus genotype 3a
... HCV NS3 genotype 3a or the full length NS5A of any genotype in protein data ...dary structure of NS3 and NS5A-T we used data from CD and FT-IR spectroscopy which indicate that both proteins fold into ... See full document
12
Substrate determinants for cleavage in cis and in trans by the hepatitis C virus NS3 proteinase.
... the hepatitis C virus polyprotein is accomplished by a series of cotranslational and posttranslational cleavages mediated by host cell signalases and two virally encoded ...the NS3 proteinase ... See full document
8
Nuclear localization of the NS3 protein of hepatitis C virus and factors affecting the localization.
... between NS3 and NS4A is considerably weak. The weak interaction between NS3 and NS4A of strain M094AJ might be explained by a structural difference in an amino-terminal portion of NS3 between HCV ... See full document
8
Crystal Structure of the NS3 Protease-Helicase from Dengue Virus
... dengue virus, a disease against which neither a vaccine nor specific antiviral therapies currently ...the C terminus of ...flavivirus NS3 polypeptide is still lacking. We report a crystallographic ... See full document
11
Polynucleotide modulation of the protease, nucleoside triphosphatase, and helicase activities of a hepatitis C virus NS3-NS4A complex isolated from transfected COS cells.
... GB hepatitis agents, suggesting that this region may be involved in genome ...that NS3 binds poly(U) extremely tightly (15, 22, 37, 42), the ability of the enzyme to translocate from this site in the genome ... See full document
9
Activity of purified hepatitis C virus protease NS3 on peptide substrates.
... the NS3 protease domain in E. coli, we chose a T7-RNA polymerase- based expression ...8 C to the desired optical density, after which the temperature was lowered to 30 8 ...of NS3 protease ... See full document
7
The NS3 proteinase domain of hepatitis C virus is a zinc-containing enzyme.
... Cu). Analysis of the metal content of the purified NS3 proteinase domain indicated the presence of a single zinc atom per molecule of enzyme which, in turn, implied the existence of a metal binding ... See full document
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