[PDF] Top 20 Structure-Function Analysis of Hepatitis C Virus Envelope-CD81 Binding
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Structure-Function Analysis of Hepatitis C Virus Envelope-CD81 Binding
... its C terminus, was fused to ...its C terminus by cellular proteases ...its C terminus, LEL was purified by immobilized metal affinity chromatogra- phy, allowing separation of the full-length LEL ... See full document
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Functional Analysis of Hepatitis C Virus Envelope Proteins, Using a Cell-Cell Fusion Assay
... HCV envelope proteins are anchored to the membrane via a single C-terminal transmembrane (TM) domain and contain an N-terminal ectodomain (36) ...the C-terminal region of the core ...the virus ... See full document
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Structure-Based Mutational Analysis of the Hepatitis C Virus NS3 Helicase
... ATP binding activity would be covalently bound to ATP and became ...specific binding of ATP to the helicase ...ATP binding ability, indicating their loss of ATPase ac- tivity, yet the other three ... See full document
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Human Monoclonal Antibodies That Inhibit Binding of Hepatitis C Virus E2 Protein to CD81 and Recognize Conserved Conformational Epitopes
... Hepatitis C virus (HCV), a member of the family Flaviviridae, expresses its proteins from a ...The virus is highly variable, with more than nine distinct genotypes (1, ...the envelope ... See full document
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Computational Prediction of the Heterodimeric and Higher-Order Structure of gpE1/gpE2 Envelope Glycoproteins Encoded by Hepatitis C Virus
... residues C terminal to the AR2 epitope belong to a set of E2 residues (residues 483 to 491 and 523 to 545) that, when mutated, were shown previously to substantially decrease the binding of the E1-specific ... See full document
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Hepatitis C Virus Induces Epidermal Growth Factor Receptor Activation via CD81 Binding for Viral Internalization and Entry
... For analysis of the effect of TGF- ␣ on expression and localization of EGFR and the HCV host receptors, cells were treated with 5 nM TGF- ␣ for 15 min with or without 1 h pretreatment with 10 M ...EEA1, ... See full document
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Pseudotyping retrovirus like particles vaccine candidates with Hepatitis C virus envelope protein E2 requires the cellular expression of CD81
... of CD81, HCV envelope proteins and MLV structural protein p30 was evaluated in all engineered cell lines and derived ...in CD81-positive and in CD81-negative cells is ...the analysis of ... See full document
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Identification of Conserved Residues in Hepatitis C Virus Envelope Glycoprotein E2 That Modulate Virus Dependence on CD81 and SRB1 Entry Factors
... on virus assembly also suggests that these res- idues are not involved in interactions with HCV glycoprotein E1, and the lack of effect on virus entry and interaction with CD81 is in agreement with ... See full document
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CD81 and Claudin 1 Coreceptor Association: Role in Hepatitis C Virus Entry
... viral envelope gp (1, 31, 69, ...between CD81 and CLDN1 and inhibited HCV infection ...between CD81-CD81, suggest- ing that the viral gps modulate CD81 association with ...E1-E2 ... See full document
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Hepatitis C Virus Hypervariable Region 1 Modulates Receptor Interactions, Conceals the CD81 Binding Site, and Protects Conserved Neutralizing Epitopes
... the hepatitis C virus (HCV), which likely contributes to immune escape, is most pro- nounced in hypervariable region 1 (HVR1) of viral envelope protein ...for virus replication in vitro ... See full document
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CD81 Is a Central Regulator of Cellular Events Required for Hepatitis C Virus Infection of Human Hepatocytes
... addition, CD81 has been shown to play a role in the infection of primary human hepatocytes by serum- derived HCV ...(45). CD81 is an integral membrane protein that shows the structure common to all ... See full document
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Characterization of the Envelope Glycoproteins Associated with Infectious Hepatitis C Virus
... or CD81 pulldown (E1 and E2 proteins), SDS-PAGE, and Western blotting with specific ...input virus preparation ...one CD81 form ( * ) during SDS-PAGE (as observed by Ponceau staining) (data not ... See full document
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Binding of the Hepatitis C Virus E2 Glycoprotein to CD81 Is Strain Specific and Is Modulated by a Complex Interplay between Hypervariable Regions 1 and 2
... single binding site only if a head-to-tail dimer is postulated, and this was indeed one of the conclusions derived from our model (44), which was once again indirectly supported by the SFV E1 ...recognizing ... See full document
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Mutations in Hepatitis C Virus E2 Located outside the CD81 Binding Sites Lead to Escape from Broadly Neutralizing Antibodies but Compromise Virus Infectivity
... (52). Analysis revealed 25 amino acid changes that are outside the HVR1 region among the variants in relationship to the reference H77c isolate (GenBank accession ...E2 structure (18), we reasoned that the ... See full document
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Hepatitis C Virus E2-CD81 Interaction Induces Hypermutation of the Immunoglobulin Gene in B Cells
... E2-CD81 binding induces activation-induced cytidine deami- nase (AID) and error-prone DNA polymerase ...E2-CD81 binding. Semi- quantitative RT-PCR analysis showed that the ... See full document
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Characterization of Hepatitis C Virus E2 Glycoprotein Interaction with a Putative Cellular Receptor, CD81
... ecule CD81 could interact with E2, suggesting that it may be the cellular receptor for ...HCV. CD81 is a member of the tetraspanin, or transmembrane 4, family, which traverses the membrane four times and ... See full document
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Different Domains of CD81 Mediate Distinct Stages of Hepatitis C Virus Pseudoparticle Entry
... The CD81 tetraspanin was first identified as a hepatitis C virus (HCV) receptor by its ability to bind the soluble ectodomain of envelope glycoprotein E2 ...that CD81 is ... See full document
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Characterization of Hepatitis C Virus (HCV) and HCV E2 Interactions with CD81 and the Low-Density Lipoprotein Receptor
... and CD81 at various time points following cell culture ...Nonspecific binding was ruled out using a mouse IgG1 isotype control antibody. CD81 was expressed at a constant level (98%), and expression ... See full document
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Tupaia CD81, SR-BI, Claudin-1, and Occludin Support Hepatitis C Virus Infection
... Hepatitis C virus (HCV)-related research has been hampered by the lack of appropriate small-animal ...Human CD81, scavenger receptor class B type I (SR-BI), claudin 1 (CLDN1), and occludin ... See full document
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Identification of the Hepatitis C Virus E2 Glycoprotein Binding Site on the Large Extracellular Loop of CD81
... and C. The inset shows the results of nonreducing SDS-PAGE analysis of peak fractions (putative MBP- LEL 113-201 dimer [circles] and MBP-LEL 113-201 monomer [triangles]) ... See full document
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