[PDF] Top 20 Structure and Leukemogenic Activity of a Murine Leukemia Virus

... Sinai School of Medicine of the City Ulliversity of New York, New York, New York 10029 Purified Friend viruses obtained from chronically infected tissue cultures were studied under the e[r] ... See full document

... Moloney murine leukemia virus (Mo-MLV) RNase H domain was performed to provide information about its structure and mechanism of ...resolution structure resembles the known structures of ... See full document

... Fragments e, f, and g, which are the carboxyl-terminal regions of fragments E, F, and G, respectively, obviously contain a carbohydrate attachment site sequence because of the size diffe[r] ... See full document

... We conclude that the elongation products of strong-stop DNA are complementary at their 5' ends to sequences present at the 5' end of the Mo-MuLV genome, complementary at their 3' ends to[r] ... See full document

... To examine the genome structure of the A-MuLV variants in transformed lymphoid cells, DNA was isolated from a variety of cloned cell lines prepared by infection of bone marrow cells in v[r] ... See full document

... Discrimination between additional amino presence of these two Asp-Pro bonds, because acid sequences and attached carbohydrates was all fragments produced by cleavage at this site made po[r] ... See full document

... DISCUSSION The data presented demonstrate that the Moloney MuLV glycoprotein precursor Pr8Oenv is a molecule with a apoprotein portion having a molecular weight of 60,000 and at least tw[r] ... See full document

... The disintegration endonuclease product results from cleavage of the LTR from the LTR-target sequence junction and can be detected with substrates having a 5'-end-labeled B strand right [r] ... See full document

... double-helical structure of the DNA at the terminus to allow access of the nucleophile to the penultimate phosphodiester ...double-helical structure of substrates in end processing (41a) and in ... See full document

... Several previous studies have outlined the organization and composition of PICs. In the case of MoMuLV, PICs are known to be large particles (around 160S) potentially containing sev- eral viral proteins (2). In the case ... See full document

... transcriptional activity in all the cell lines stud- ied, even in a Friend erythroleukemia cell ...the leukemogenic potential of the virus in the corresponding tissue has been suggested (3, 4, ... See full document

... Studies on the infectivities of MXMRV vs XMRV in different human cells indicated that some cell lines (e.g. HeLa) showed substantially higher susceptibility/ infectibility for MXMRV than for XMRV (≥ 20-fold, Table 3), ... See full document

... 4242-4251 0022-538X/90/094242-10$02.00/0 Copyright © 1990, American Society for Microbiology Temperature-Sensitive Mutants of Abelson Murine Leukemia Virus Deficient in Protein Tyrosine [r] ... See full document

... The AMuLV strains encoding P160, P120, P100, and P90 all transform fibroblasts, and high-titer transforming virus stocks are obtained from using the agar transformation assay 11 and BALB[r] ... See full document

... These results show that at the time of embryo transfer the BXH-2 V- mice had not acquired additional endogenous ecotropic proviruses in the germ line and that in the BXH-2 strain the exo[r] ... See full document

... 2843 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest On the basis of earlier studies with both detergent-disrupted virions the endogenous reaction and an in vitro recon[r] ... See full document

... of leukemia can be further distin- guished by identifying the phenotypes of the cells responsible for the gross spleen ...esterase activity, a marker of the erythroid lineage, and Sudan black-positive ... See full document

... Failure of the eco- and the xenotropic virus isolates to induce leukemia did not depend on the dose of virus inoculated or on the route of inoculation; inoculation of high titers 106 of [r] ... See full document

... Intraperitoneal inoculation of Mo+PyF101 M-MuLV led to high levels of infectious centers in bone marrow, comparable to findings for animals infected with wild-type M-MuLV as early as 10 [r] ... See full document

... B DEAE-Sepharose column fractions from virus disrupted with 0.5 M KCI and detergent: lanes A and B contained most of the reverse transcriptase activity; lanes B and C contained most of t[r] ... See full document