[PDF] Top 20 Surface Loop Dynamics in Adeno-Associated Virus Capsid Assembly

... HI loop is a prominent domain on the adeno-associated virus (AAV) capsid surface that extends from each viral protein (VP) subunit overlapping the neighboring fivefold ...unique ... See full document

... Additional amino acids located in close proximity to N470 are required for AAV9 galactose binding. Additional AAV9 mu- tants were generated to determine whether any of the amino acids structurally surrounding N470 are ... See full document

... AAV1 capsid VP3 monomer (PDB ID, ...rior capsid surfaces are labeled. (B) Radially color-cued (from capsid center to surface, blue to green to yellow to red) surface representation of ... See full document

... of virus with acidic pH in vitro does not in itself induce exposure of the VP1 N terminus, passage through acidic endosomes appears to be essential ...minute virus of mice (MVM) undergoes cleavage of its ... See full document

... with capsid- specific antibodies and was confirmed by visualization of the pro- teins in the 60S fractions by electron microscopy ...rather surface interactions as they occur at the 3-fold and 5-fold ... See full document

... become surface exposed when capsids are heated, suggest- ing that this may be mimicking what is happening within the cell to expose the phospholipase domain of Vp1 (2, ...become surface exposed on the ... See full document

... the adeno-associated virus capsid allows researchers to understand capsid domains and develop efficient vectors for gene ...HI loop, a previously characterized capsid ... See full document

... 3 Capsid lumenal surface charge influences AAP-VP assembly ...lumenal surface with each monomer differentially ...E) Surface charge predictions for (C) Anc81, (D) the Anc81 E413Q single ... See full document

... AAV capsid shell is assembled from 60 copies (in total) of viral proteins (VP), VP1 ( ⬃ 87 kDa), VP2 ( ⬃ 73 kDa), and VP3 ( ⬃ 61 ...AAV capsid includes 2-, 3-, and 5-fold symmetric interactions among the VP ... See full document

... abundant capsid proteins, VP1 (⬃87 kDa) and VP2 ( ⬃ 73 kDa), containing unique N-terminal exten- sions (VP1u) containing a phospholipase A2 (PLA2) domain and nuclear localization signals (VP1/2 common region), ... See full document

... events associated with endosomal processing of the parvovirus capsid, such as the externalization of VP1u and VP2 N termini or genome uncoating, since the 5-fold pore is predicted to be a portal for these ... See full document

... apical surface of differentiated human airway epithelia but achieve poor transgene expression, in part due to ubiquitination of the AAV capsid after virion internalization and endosomal release ... See full document

... Interestingly, although the large Rep protein of B-AAV, like its AAV2 counterpart, functions as a transcriptional activator, it has no obvious role in the processing of B-AAV P41-gener- ated RNA. This is in contrast to ... See full document

... during virus production, they serve as rescue signals mediating excision of the embedded genome from the vector plasmid, before initiating amplification of the rescued DNA, by assuming a hairpin shape and ... See full document

... virion surface contains highly repetitive se- quences or carbohydrates, a TI B cell response can be induced from AAV infection only via intraspleen injection in mice ...AAV capsid mounts a very strong TD ... See full document

... Early stages of infection with mixed virions. When cells were transfected with mutant proviral DNA alone, the amounts of particles released were too small to determine if the mutants were functional for early steps of ... See full document

... of adeno- virus, implying that AAV alone is capable of penetration into the cytosol, it was of interest to determine synergism or com- petition of endosomal escape following adenovirus and AAV ... See full document

... AAV1 capsid respon- sible for its ability to transduce skeletal muscle by systemati- cally exchanging domains between AAV1 and ...entire capsid genome and thus there are numerous regions where homologous ... See full document

... inhibited capsid assem- bly could be due to the absence of sequences required for triplex formation or to the lack of sequences involved in at- tachment of the triplex to the major capsid-scaffold protein ... See full document

... examined capsid cell entry and nuclear trafficking in the presence of Ad coinfection by confocal microscopy as a function of time ...Wild-type virus was observed entering the nucleus as early as 8 h ... See full document