[PDF] Top 20 T-cell activation is required for efficient replication of human herpesvirus 6.
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T-cell activation is required for efficient replication of human herpesvirus 6.
... Most notably, the yield of infectious virus was 2 to 3 logs lower in the T-cell cultures which were treated with PHA in the absence of exogenous IL-2, indicating that the addition of mit[r] ... See full document
5
Human cytomegalovirus, human herpesvirus 6 and human herpesvirus 7 infection of the immunocompromised host
... and T-cell activation genes, such as those of IL-2 and the IL-2 receptor, is ...IL-2-sdmulated T-cell proliferation and the generation of antigen-specific cytotoxic ...the ... See full document
215
The simultaneous presence and expression of human hepatitis C virus (HCV), human herpesvirus 6 (HHV 6), and human immunodeficiency virus 1 (HIV 1) in a single human T cell
... Our TEM analysis showed HHV-6A and HCV in the same cells, with extracellular HIV-1 particles budding or adja- cent to the infected cells. Since HIV-1 assembly is com- pleted at the plasma membrane level, their presence ... See full document
8
T-Cell Growth Transformation by Herpesvirus Saimiri Is Independent of STAT3 Activation
... receptor activation, they are recruited to a receptor where they are tyrosine phosphor- ylated by receptor tyrosine kinases (RTK) or receptor-associ- ated tyrosine kinases, such as JAK or Trk kinases (reviewed in ... See full document
8
The Cellular Protein SPT6 Is Required for Efficient Replication of Human Cytomegalovirus
... different cell types or expression vectors in the heterokaryon ...viral replication, UL69 shuttling activity, SPT6 binding, or UL69’s ability to interact with eIF4a1, URH49, or wild-type ... See full document
10
Viral Replication-Independent Blockade of Dendritic Cell Maturation and Interleukin-12 Production by Human Herpesvirus 6
... 2% human serum at 250 ⫻ g for 5 min and then incubated for 30 min on ice with primary ...2% human serum, the cells were incubated for an addi- tional 30 min with the appropriate secondary antibody ... See full document
7
Host Cell Nucleolin Is Required To Maintain the Architecture of Human Cytomegalovirus Replication Compartments
... viral replication compartments, the subnuclear structures in which the viral DNA genome is ...of replication compartments is poorly ...with human cytomegalovirus (HCMV) showed that the viral DNA ... See full document
11
Immediate Activation Fails To Rescue Efficient Human Immunodeficiency Virus Replication in Quiescent CD4+ T Cells
... DNA PCR was performed as previously described (21) using a primer-probe pair that amplifies cellular -globin sequences and one that amplifies full-length HIV reverse transcripts (the long terminal repeat-gag junction), ... See full document
9
Human Herpesvirus 6 Suppresses T Cell Proliferation through Induction of Cell Cycle Arrest in Infected Cells in the G2/M Phase
... HHV-6 replication is CD4 ⫹ T lymphocytes, a pivotal cell type in the generation of humoral and cell-mediated adaptive immune ...as human immunodeficiency virus ...induces ... See full document
10
Regulation of the herpesvirus saimiri oncogene stpC, similar to that of T-cell activation genes, in growth-transformed human T lymphocytes.
... formed human T ...formed T cells appear to utilize stimulation signals in order to influence both the Lck- and Ras-dependent signal transduction pathways via increased expression of Tip and ... See full document
8
TCR-triggered extracellular superoxide production is not required for T-cell activation
... + T cells were purified from peripheral blood mononuclear cells by non-T cell depletion using human naïve CD4 + T-cell isolation kit and AutoMACS (all from Miltenyi ...Biotec). ... See full document
10
Herpesvirus Saimiri-Transformed Human CD4+ T-Cell Lines: an Efficient Target Cell System for the Analysis of Human Immunodeficiency Virus-Specific Cytotoxic CD8+T-Lymphocyte Activity
... against human immunodeficiency virus (HIV) infection (3, 25, ...(5, 6, 13, 15, 16, 22, 29, 30, ...target cell system allows the detection of a CTL activity restricted by the complete set of a ... See full document
5
Capsid coding sequence is required for efficient replication of human rhinovirus 14 RNA.
... RPA. The RNase protection assay (RPA) was carried out with reagents and protocols supplied with the lysate RNase protection kit (United States Biochemi- cals). Transfected-cell monolayers in 60-mm dishes were ... See full document
12
Rapamycin-Resistant mTORC1 Kinase Activity Is Required for Herpesvirus Replication
... in cell lysates were resolved on 10% SDS-contain- ing polyacrylamide gels, with the following exceptions: for 4EBP1 blots, lysates were resolved on 15% gels, and for eIF4G analysis, lysates were resolved on 8% ... See full document
10
Downregulation of the T-Cell Receptor Complex and Impairment of T-Cell Activation by Human Herpesvirus 6 U24 Protein
... ⫹ T cells, and further study of the role of U24 in roseolavirus infection is ...downregulate cell surface B-cell receptor BCR expression by blocking its transport to the cell surface ...(30). ... See full document
7
The Human Herpesvirus 6 G Protein-Coupled Receptor Homolog U51 Positively Regulates Virus Replication and Enhances Cell-Cell Fusion In Vitro
... HHV-6-susceptible T cells prior to exposing the cells to infectious ...the replication and attach- ment of other human herpesviruses ...HHV-6 replication and cytopathic effect in ... See full document
11
Herpesvirus Interference with Major Histocompatibility Complex Class II-Restricted T-Cell Activation
... two human gammaherpesviruses (22, ...II-restricted T-cell activation, can now be explored for cells undergoing replication of these ...EBV replication, a reduction in the sur- ... See full document
8
Human Herpesvirus 6 Infects Dendritic Cells and Suppresses Human Immunodeficiency Virus Type 1 Replication in Coinfected Cultures
... are required; HHV-6 MOIs of ,0.001, heat-inactivated HHV-6, and HHV- 6 neutralized with MAbs fail to suppress HIV replication ...(Fig. 6 and ...HIV replication in DC when ... See full document
10
TORC1 and TORC2 Coactivators Are Required for Tax Activation of the Human T-Cell Leukemia Virus Type 1 Long Terminal Repeats
... Plasmids. Human cDNAs containing complete coding regions for TORC1, TORC2, and TORC3 were derived from expressed sequence tag clones (IMAGE clone identification ...(5, 6, ... See full document
8
Interactome Analysis of the Influenza A Virus Transcription/Replication Machinery Identifies Protein Phosphatase 6 as a Cellular Factor Required for Efficient Virus Replication
... infected cell is necessary for elucidating the molecular mechanisms that under- lie virus ...host cell (reviewed in references 10, 73, and ...host cell nucleus and recruit host factors to enable them ... See full document
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