[PDF] Top 20 Targets of Small Interfering RNA Restriction during Human Immunodeficiency Virus Type 1 Replication

... genomic RNA but not the incoming HIV-1 genomic RNA, suggesting that this protection from siRNA degradation is related to viral ...capsid. During coevolu- tion of pathogen and host, various ... See full document

... Human immunodeficiency virus type 1 (HIV-1) gene expression is regulated by both cellular transcription factors and ...TAR RNA in conjunction with cyclin T1 and ... See full document

... role during human immunodeficiency virus (HIV) ...However, during late stages of infection, the viral receptor exerts inhibitory effects on the infectivity of the released particles ... See full document

... esiRNAs generated by Dicer more efficiently inhibit HIV-1 replication. To establish an alternative method for generating esiRNA libraries, we explored the ability of the enzyme Dicer to substitute for the ... See full document

... the human immunodeficiency virus type 1 (HIV-1) was first isolated 30 years ago, tremendous progress has been made in the prevention and treatment of ...novel targets and ... See full document

... the human immunodeficiency virus type 1 genome requires several cellular factors regulating transcription, alternative splicing, RNA stability, and intracellular localization of ... See full document

... Marburg virus-infected cells, suggesting that Rab9 is utilized in Marburg ...in human immunodeficiency virus (HIV) replication, previous reports suggested that the late endosome is an ... See full document

... various virus clones in Fig. 1, input cell-free virus samples were prepared from transfected 293T cells and inoculated into the cell lines indicated in Table ...Three restriction enzymes ... See full document

... of human immunodeficiency virus type 1 (HIV-1) infections occur via mucosal contact (40), and there are several reports indicating that the oral mucosa may be one route of ... See full document

... HIV-1 replication in some rodent cell lines. At least one restriction in cell lines from mice and hamsters is the inability of the viral transcription regulator, Tat, to activate transcription from ... See full document

... potential targets for ...viral RNA and the viral replication [25]. Virus resistance has been proven to be generated by RNAi triggered from exogenous DEN-specific ...the type II dengue ... See full document

... the virus to escape siRNA ...its RNA genome ...C virus replicons ...a small region of high tolerability for mismatches at the 3 ⬘ and 5 ⬘ ends of the target sequence and a region of fairly low ... See full document

... amount of ␤ -galactosidase produced via the viral LTR pro- moter using a chemiluminescent substrate (Gal Screen). The distribution of signals from all of the siRNAs and controls is shown in Fig. 1B, where the positive ... See full document

... and RNA-packaging defect of the DIS mutants is rela- tively minor, we wanted to obtain additional evidence for the loss of infectivity of these ...produced virus stocks revealed an approximately 10-fold re- ... See full document

... the replication of all 47 HIV-1 laboratory strains and clinical isolates examined with a 50% inhibitory concentration of ⬃ 10 ␮ M, a concentration that is 200- to 2,000-fold less than what affected cell ... See full document

... by small interfering RNAs (siRNAs), has been demonstrated in recent studies to be an effective strategy against human immunodeficiency virus type 1 ...viral RNA. ... See full document

... Current experimental data to support the role of hA3G deamination of DNA in either the degradation of viral DNA or in any other antiviral activity remain inconclusive. Since the deamination mutations in DNA result in the ... See full document

... HIV-1 replication in infants and ...HIV-1 RNA copy number following the initiation of antiretroviral therapy allowed the estimation of HIV-1 kinetic ... See full document

... No depletion of CD4+ human thymocytes was observed in implants infected with either HIV-lJRCSF or its nef mutant at 3 or 6 weeks postinfection compared with controls consisting of mock-i[r] ... See full document

... As astrocytic glial cells supported TAR-independent gene expression from an HIV-1 LTR in subgenomic reporter constructs, we evaluated whether unstimulated astrocytic glial cells could su[r] ... See full document