[PDF] Top 20 The C Terminus of the Herpes Simplex Virus UL25 Protein Is Required for Release of Viral Genomes from Capsids Bound to Nuclear Pores
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The C Terminus of the Herpes Simplex Virus UL25 Protein Is Required for Release of Viral Genomes from Capsids Bound to Nuclear Pores
... a C-terminally truncated pUL25 protein ...extending from a rigid core composed of several tightly packed alpha helices ...three C-terminal amino acids removed one of the six unstructured loops ... See full document
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Residues of the UL25 Protein of Herpes Simplex Virus That Are Required for Its Stable Interaction with Capsids
... The herpes simplex virus 1 (HSV-1) UL25 gene product is a minor capsid component that is required for encapsidation, but not cleavage, of replicated viral ...DNA. UL25 is ... See full document
13
A Domain of Herpes Simplex Virus pUL33 Required To Release Monomeric Viral Genomes from Cleaved Concatemeric DNA
... released from the concatemer. Because type A capsids that result from aborted packaging events were not detected, it is likely that the U L 33 mutants abort the packaging reaction after DNA cleavage ... See full document
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Role of the UL25 Protein in Herpes Simplex Virus DNA Encapsidation
... The herpes simplex virus protein UL25 attaches to the external vertices of herpes simplex virus type 1 capsids and is required for the stable ... See full document
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The UL14 Tegument Protein of Herpes Simplex Virus Type 1 Is Required for Efficient Nuclear Transport of the Alpha Transinducing Factor VP16 and Viral Capsids
... Electron microscopy. Cells were grown to monolayers on sapphire disks coated with carbon and infected with wild-type or UL14D virus. At 16 to 24 h postin- fection, the cells were washed in mammalian Ringer ... See full document
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The UL25 Gene Product of Herpes Simplex Virus Type 1 Is Involved in Uncoating of the Viral Genome
... ts1249 capsids by immunofluorescence revealed that ts1249 was able to enter the cell at the NPT, and this result was supported by the finding that cells coinfected with ts1249 and the defective virus in1383 ... See full document
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Uncoupling Uncoating of Herpes Simplex Virus Genomes from Their Nuclear Import and Gene Expression
... Incoming capsids of herpes simplex virus type 1 (HSV-1) enter the cytosol by fusion of the viral envelopes with host cell membranes and use microtubules and microtubule motors for ... See full document
13
Residues of VP26 of Herpes Simplex Virus Type 1 That Are Required for Its Interaction with Capsids
... the C terminus of the molecule, which encompasses a region of highly conserved residues, abolished binding to the capsid and the localization of GFP to the nucleus in characteristic large ...spanning ... See full document
14
Role of Herpes Simplex Virus 1 Immediate Early Protein ICP22 in Viral Nuclear Egress
... the nuclear capsids is signifi- cantly ...exit from the perinuclear space. This suggests that the rate of viral egress from the nucleoplasm decreases in the absence of ICP22 compared to ... See full document
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Release of the catalytic domain N(o) from the herpes simplex virus type 1 protease is required for viral growth.
... m100 virus, ...B capsids (6, 11, 44), plays a structural role in addition to its enzymatic activity in the capsids, and this role can be achieved only after release from ... See full document
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The Amino Terminus of Herpes Simplex Virus 1 Glycoprotein K (gK) Is Required for gB Binding to Akt, Release of Intracellular Calcium, and Fusion of the Viral Envelope with Plasma Membranes
... enveloped capsids or by fusion of the viral envelope with cellular membranes, or by both ...of viral envelopes with cellular plasma mem- branes is associated with binding to phosphorylated Akt and ... See full document
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Functional inaccessibility of quiescent herpes simplex virus genomes
... the viral gene expression program subsequent to the immediate-early phase ...eGFP from the HCMV IE pro- moter does not necessarily imply productive infection with ...of viral DNA repli- cation ... See full document
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Proteolytic Cleavage of the Amino Terminus of the UL15 Gene Product of Herpes Simplex Virus Type 1 Is Coupled with Maturation of Viral DNA into Unit-Length Genomes
... r protein was the predominant form detected in capsid-containing frac- ...B capsids from Vero cells infected with HSV-1(F), HSV-1(⌬U L 17), hr64 (U L 32⫺), and Cos-U L 33⫺ (panels A, B, C, and ... See full document
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Herpes Simplex Virus Type 1 UL34 Gene Product Is Required for Viral Envelopment
... 34 protein with membranes in the infected cell and its expression as a gamma-1 gene suggest a role in maturation or egress of the virus particle from the ...recombinant virus that fails to ... See full document
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Nucleolin Is Required for Efficient Nuclear Egress of Herpes Simplex Virus Type 1 Nucleocapsids
... recombinant virus expressing capsid protein VP26 fused to an FP showed that the number of mature capsids in the cytoplasm and at the surface of Vero-shNuc cells was significantly lower than in ... See full document
12
Herpes Simplex Virus Type 1 DNA-Packaging Protein UL17 Is Required for Efficient Binding of UL25 to Capsids
... UL17, UL25, and UL6, only UL17 and UL6 appear to be components of the procapsid, with UL25 being added ...or UL25 with capsids was dependent on the other two packaging proteins, B ... See full document
9
Assembly of herpes simplex virus capsids using the human cytomegalovirus scaffold protein: critical role of the C terminus.
... the C-terminal end through proteolytic cleavage by the protease prior to capsid ...complete capsids and have used this correlation to define the minimal domain within the C terminus of the ... See full document
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The Product of the Herpes Simplex Virus Type 1 UL25 Gene Is Required for Encapsidation but Not for Cleavage of Replicated Viral DNA
... wild-type virus in the mutant stocks is due to recombination that occurs between homologous se- quences present in the mutant viral genome and the wild-type sequences in the transformed cell ...KUL25NS ... See full document
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The N Terminus and C Terminus of Herpes Simplex Virus 1 ICP4 Cooperate To Activate Viral Gene Expression
... Activate Viral Gene Expression Lauren ...transcription from most viral ...one C terminal, are largely responsible for the activation functions of ...the C-ter- minal activation domain ... See full document
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Structural Characterization of the UL25 DNA-Packaging Protein from Herpes Simplex Virus Type 1
... DNA genomes as high-molecular-weight concatemers which are subsequently cleaved into unit-length genomes by a complex mechanism that is tightly coupled to DNA insertion into a preformed capsid structure, ... See full document
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