[PDF] Top 20 Three-Dimensional Stem Cell Bioprinting

... Although stem cells are capable of regenerating tissues, current research trends tend to side on developing fully functional organs and other clinical uses including in situ stem cell repair through ... See full document

... gland-resident stem cells (SGSCs) are salivary gland-resident tissue stem cells which are essential for salivary gland homeostasis and regeneration ...into three-dimensional (3D) spheroid-like ... See full document

... The formation of newly formed blood vessels within tissue-engineered skin substitutes is a key factor in evalu- ating their vascularization capability. CD31, as a trans- membrane protein, is a good marker of endothelial ... See full document

... of stem cells was used to increase the implanted ADSC retention in the infarcted ...enhanced cell retention ...transplantation. Three-dimensional cell masses (3DCMs), a type of spheroid ... See full document

... pluripotent stem cells to create three-dimensional skin equivalents for drug ...pluripotent stem cells (iPSCs), from which various cell types (including fi broblasts and keratinocytes) ... See full document

... for processing and isolation of cells. More work needs to be done on the molecular characterization of these cells and how the effect of the tissue they originate from affects their commitment. As Shafiee et al. ... See full document

... breast-cancer stem-like cells (BCSC), express markers similar to mammary stem cells, and are highly resistant to ...and stem-like properties of breast cancer cell lines grown on nanofiber ... See full document

... the cell culture reached 70% confluence, the com- plete culture medium was replaced with a specific differen- tiation medium that contained 90% StemPro Osteocyte/ Chondrocyte Differentiation Basal Medium ... See full document

... cells could survive and mature gradually in vivo. However, some grafted cells clumped together in the subretinal space. The absence of Ki67 immunostaining suggested transplanted 3D-RPE-60d cells were quiescent 4 weeks ... See full document

... postnatal stem cell therapy, (c) pulp implantation, (d) scaffold implantation, (e) injectable scaffold delivery, (f ) three-dimensional cell printing, and (g) gene ... See full document

... mesenchymal stem cell-conditioned media enhanced the proliferation of endothelial stem cells in a dose-de- pendent manner, and anti-vascular endothelial growth factor antibodies partly attenuated ... See full document

... Characterization of the MSC immunophenotype was per- formed as described previously [7]. Continuously proliferat- ing MSC were harvested and analyzed for cell surface marker expression by flow cytometry. After ... See full document

... 3D cell culture due to its simplicity and homogeneity of the cultured spheroids [75, ...the cell spheroids formed in such platform are believe to closely resemble in vivo tissues [75, ...the cell ... See full document

... natural cell microenvironment; however, it is equally necessary that the gels maintain cell viability and phenotype while permitting reisolation without stress, especially for use in the stem ... See full document

... of cell biology and engineering, providing an environment in which cells and bioactive molecules can interact synergistically in order to promote tissue ...tailored three-dimensional scaffolds, that ... See full document

... RT-PCR was used to evaluate the expression of albumin (ALB), Alpha-fetoprotein (AFP), Tyrosine amino- transferase (TAT), and cytochrome P4507A1 (CYP7A1) in differentiated cells on the scaffold. Total RNA was isolated ... See full document

... of cell-laden hydrogel constructs for vascular applications ...that three-armed polycaprolactone (PCL) oligomers of various molecular weights were synthesized, end-functionalized with methacrylic an- ... See full document

... ml −1 in six-well plates and were allowed to attach and acquire normal morphology. Then the cells were transduced using a lentiviral vector which expresses enhanced green fl uorescent protein under control of the SFFV ... See full document

... After three days all cell cultures formed spheroid, three- dimensional cell units in high density (5 × 10 6 cells/ml), which appeared as oval ... See full document

... direct cell–cell contact is not neces- sary for phenotypic changes in MSC ...direct cell–cell contact resulting in an interaction effect [20, ...that stem cell differentiation ... See full document