[PDF] Top 20 A View of the E2-CD81 Interface at the Binding Site of a Neutralizing Antibody against Hepatitis C Virus
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A View of the E2-CD81 Interface at the Binding Site of a Neutralizing Antibody against Hepatitis C Virus
... ABSTRACT Hepatitis C virus (HCV) glycoprotein E2 is considered a major target for generating neutralizing antibodies against HCV, pri- marily due to its role of engaging host ... See full document
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Hepatitis C Virus Envelope Glycoprotein E2 Glycans Modulate Entry, CD81 Binding, and Neutralization
... of E2 glycan re- moval on antibody ...of E2, indicating that this do- main is exposed on the virion ...the CD81 binding site, rendering it more accessible to CD81 as well ... See full document
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Mutations in Hepatitis C Virus E2 Located outside the CD81 Binding Sites Lead to Escape from Broadly Neutralizing Antibodies but Compromise Virus Infectivity
... broad neutralizing profile of this antibody against different genotypes and subtypes ...a site of variability at residue 431 on E2 that can lead to escape variants ...to ... See full document
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Identification of a Broadly Cross-Reacting and Neutralizing Human Monoclonal Antibody Directed against the Hepatitis C Virus E2 Protein
... e137 binding of the panel of E1-E2 mutants ...HCV E2 regions, since substitu- tions at conserved positions 416, 420, 529, 530, and 535 re- duced binding by greater than ...for CD81 ... See full document
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Hepatitis C Virus Hypervariable Region 1 Modulates Receptor Interactions, Conceals the CD81 Binding Site, and Protects Conserved Neutralizing Epitopes
... in virus-producing ...an E2-specific monoclonal antibody (AP33), or a polyclonal E1-specific antiserum and subjected to endo- -N- acetylglucosaminidase H (Endo H) ...the E2 protein which was ... See full document
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Mapping a Region of Hepatitis C Virus E2 That Is Responsible for Escape from Neutralizing Antibodies and a Core CD81-Binding Region That Does Not Tolerate Neutralization Escape Mutations
... broadly neutralizing HMAbs to overlapping epitopes of the E2 protein, which we designated domain B (21, 22, ...one antibody in particular that completely neutralized the diversity of HCV quasispecies ... See full document
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Identification and Characterization of Broadly Neutralizing Human Monoclonal Antibodies Directed against the E2 Envelope Glycoprotein of Hepatitis C Virus
... An extensive search of the HCV database identified a total of 28 mutations in the critical residues of the HCV1 and 95-2 epitope of the E2 glycoprotein. Interestingly, HCVpp could be generated for only five of ... See full document
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Neutralizing Monoclonal Antibodies against Hepatitis C Virus E2 Protein Bind Discontinuous Epitopes and Inhibit Infection at a Postattachment Step
... tide binding (9, 18), phage display (65), alanine-scanning mu- tagenesis of recombinant E1-E2 (33, 37, 49, 62) or E2 (34), or generation of neutralization escape mutants (19) to localize ... See full document
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Naturally selected hepatitis C virus polymorphisms confer broad neutralizing antibody resistance
... testing against diverse, representative panels of HCV ...characterize neutralizing breadth and resistance mechanisms of 18 previously described broadly neutralizing anti-HCV human ...in E2 ... See full document
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Antibody Response to Hypervariable Region 1 Interferes with Broadly Neutralizing Antibodies to Hepatitis C Virus
... the E2 glycoprotein of hepatitis C virus contributes to persistent infection by evolving escape mutations that attenuate binding of inhibitory antibodies and by blocking access of ... See full document
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Nonneutralizing Antibodies to the CD4-Binding Site on the gp120 Subunit of Human Immunodeficiency Virus Type 1 Do Not Interfere with the Activity of a Neutralizing Antibody against the Same Site
... infectious virus encounters its cellular ...virion- binding assays as surrogates for neutralization assays (39, 48), although we have not addressed these issues experimentally ...contain binding ... See full document
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Identification of the Hepatitis C Virus E2 Glycoprotein Binding Site on the Large Extracellular Loop of CD81
... myc binding was measured in a gamma counter following incubation with 125 I-labeled MAb 9E10 and washing of the ...entire CD81 molecule completely abolished E2 661 myc-binding ability while ... See full document
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Binding of Hepatitis C Virus E2 Glycoprotein to CD81 Does Not Correlate with Species Permissiveness to Infection
... the CD81 molecule from a primate genus (Sagui- nus) nonsusceptible to the infection ...the CD81 open reading frame was performed using primers com- plementary to the h-CD81 sequence, and the product ... See full document
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Identification of Conserved Residues in the E2 Envelope Glycoprotein of the Hepatitis C Virus That Are Critical for CD81 Binding
... CD4 binding (37), resi- dues whose involvement was later confirmed when the crystal structure of gp120 was obtained ...in CD81 binding may exhibit amino acid hetero- geneity that is contributed by ... See full document
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Expression and Characterization of a Minimal Hepatitis C Virus Glycoprotein E2 Core Domain That Retains CD81 Binding
... in binding to recom- binant dimeric MBP-LEL 113-201 for singly and multiply deleted E2 661 myc constructs, differences in binding to surface-ex- pressed CD81 were observed, perhaps reflecting ... See full document
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CD81-Dependent Binding of Hepatitis C Virus E1E2 Heterodimers
... 2003 Hepatitis C virus (HCV) is the leading cause of chronic liver disease ...recombinant E2 envelope proteins, used as surrogates for viral particles, were shown to bind specifically to human ... See full document
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Monoclonal Antibody AP33 Defines a Broadly Neutralizing Epitope on the Hepatitis C Virus E2 Envelope Glycoprotein
... AP33 antibody is highly conserved across all the major geno- types and that this antibody is capable of broad neutralization, at least in the HCVpp ...of neutralizing infection by patient-derived ... See full document
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Structure-Function Analysis of Hepatitis C Virus Envelope-CD81 Binding
... 2000 Hepatitis C virus (HCV) is a major human pathogen causing chronic liver ...human CD81 binds ...recombinant E2 protein and a recombinant soluble form of CD81 ...binds ... See full document
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Human Monoclonal Antibodies That Inhibit Binding of Hepatitis C Virus E2 Protein to CD81 and Recognize Conserved Conformational Epitopes
... HCV E2 with CD81 were obtained from an HCV-infected individual with a high- titer immune response to ...HCV E2 and CD81 in HCV infection. More impor- tantly, if CD81 is confirmed to be ... See full document
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Characterization of Hepatitis C Virus E2 Glycoprotein Interaction with a Putative Cellular Receptor, CD81
... of E2 661 . The open reading frame encoding E2 661 was PCR amplified from an existing cDNA cloned sequence (pBRTM/HCV1-3011; gift from ...the C-terminal epitope tag is ... See full document
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