[PDF] Top 20 In Vitro Analysis of Human Immunodeficiency Virus Type 1 Minus-Strand Strong-Stop DNA Synthesis and Genomic RNA Processing
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In Vitro Analysis of Human Immunodeficiency Virus Type 1 Minus-Strand Strong-Stop DNA Synthesis and Genomic RNA Processing
... the RNA- DNA heteroduplex and then reassociate, potentially undergo- ing multiple rounds of DNA synthesis and/or RNA cleavage on either the same or different ...of DNA ... See full document
15
Template Dimerization Promotes an Acceptor Invasion-Induced Transfer Mechanism during Human Immunodeficiency Virus Type 1 Minus-Strand Synthesis
... FIG. 1. RNA templates used in the study and their dimerization ...the genomic RNA that correspond to the donor and acceptor RNA templates are indicated, along with the positions of the ... See full document
12
A dual role of the putative RNA dimerization initiation site of human immunodeficiency virus type 1 in genomic RNA packaging and proviral DNA synthesis.
... impaired minus-strand DNA synthesis corresponding to the 5 9 long terminal repeat ...domain. Synthesis of complete minus-strand DNA was not significantly altered ... See full document
7
Roles of the Human Immunodeficiency Virus Type 1 Nucleocapsid Protein in Annealing and Initiation versus Elongation in Reverse Transcription of Viral Negative-Strand Strong-Stop DNA
... The human immunodeficiency virus type 1 (HIV-1) nucleo- capsid protein (NC) is processed from Gag precursors and is able to bind tightly to viral genomic RNA (2, 6, ... See full document
6
Human immunodeficiency virus Type 1 nucleocapsid protein (NCp7) directs specific initiation of minus-strand DNA synthesis primed by human tRNA(Lys3) in vitro: studies of viral RNA molecules mutated in regions that flank the primer binding site.
... the RNA template must be prerequisite for correct annealing between these struc- tures and in order for efficient reverse transcription to ...viral RNA and the tRNA ...bears strong similarity to ... See full document
9
Processing of Lagging-Strand Intermediates In Vitro by Herpes Simplex Virus Type 1 DNA Polymerase
... viral DNA replication to take place in discrete structures within the nuclei of infected cells provided an op- portunity to determine whether or not Fen-1 could distribute to areas of active viral ... See full document
14
Cis-acting Elements Important for Potato Virus X Minus-strand RNA Synthesis In Vitro and In Vivo
... 1993(b)). DNA and RNA polymerases not only have a similar protein structure, they but also share several conserved motifs in the palm ...extend DNA/RNA strand by addition of new ...of ... See full document
184
Actinomycin D Inhibits Human Immunodeficiency Virus Type 1 Minus-Strand Transfer in In Vitro and Endogenous Reverse Transcriptase Assays
... on human immunodeficiency virus type 1 (HIV-1) reverse transcription during the course of studies with a reconstituted system (re- ferred to here and in reference 28 as an in ... See full document
9
Sequence and structural determinants required for priming of plus-strand DNA synthesis by the human immunodeficiency virus type 1 polypurine tract.
... [Table 1]) from the R-Pu/D-Py class (25) and tested for primer activity with HIV-1 ...densitometric analysis of the extension products produced with HIV-1 RT ...lanes 1 and 9] and B ... See full document
9
Zinc Finger Structures in the Human Immunodeficiency Virus Type 1 Nucleocapsid Protein Facilitate Efficient Minus- and Plus-Strand Transfer
... in vitro studies, we have also examined the phenotype of viral particles carrying the SSHS mutation in both zinc fingers of the NC domain in ...virion RNA, and were unable to synthesize detectable levels of ... See full document
9
Nef stimulates human immunodeficiency virus type 1 proviral DNA synthesis.
... of virus particle formation, as the infectivity of D Nef virus could be enhanced by providing the protein in virus producer but not target ...viral DNA de- tectable in cells infected with ... See full document
9
Human Immunodeficiency Virus Type 1 Nucleocapsid Protein Can Prevent Self-Priming of Minus-Strand Strong Stop DNA by Promoting the Annealing of Short Oligonucleotides to Hairpin Sequences
... in 1⫻ RT binding buffer (50 mM Tris-Cl, [pH 8.0], 80 mM KCl, 1 mM dithiothreitol, 100 M bovine serum albumin) at a concentration of 30 M and stored in 4-l aliquots in 150-l tubes at ...template ... See full document
8
Human immunodeficiency virus type 1 nucleocapsid protein promotes efficient strand transfer and specific viral DNA synthesis by inhibiting TAR-dependent self-priming from minus-strand strong-stop DNA.
... viral DNA syn- ...3, DNA products smaller than (2) SSDNA (95 to 102 nt) with the characteristics of SP DNAs can be ...When synthesis of these small SP DNAs is inhibited by incubation with actino- ... See full document
11
Effects of 3'-deoxynucleoside 5'-triphosphate concentrations on chain termination by nucleoside analogs during human immunodeficiency virus type 1 reverse transcription of minus-strand strong-stop DNA.
... at 1/2 and 1/10 of native dNTP concentrations; however, 3TC-TP and ddITP resulted in lower efficiencies of chain termination than the others did ...cellular synthesis of ddITP ...of ... See full document
9
Requirements at the 3′ End of the Sindbis Virus Genome for Efficient Synthesis of Minus-Strand RNA
... vaccinia virus expressing the polyprotein P123, but not nsP4, were used to demonstrate that RNA synthesis in the reactions was dependent upon the presence of the SIN RdRp ...lane 1). The ... See full document
10
Discontinuous plus-strand DNA synthesis in human immunodeficiency virus type 1-infected cells and in a partially reconstituted cell-free system.
... HIV-1 RNA and incubations proceed until the formation of double-stranded ...viral DNA synthesis: (i) minus-strand synthesis by RT, (ii) cleavage of RNA-DNA ... See full document
11
Mutations in the Human Immunodeficiency Virus Type 1 Polypurine Tract (PPT) Reduce the Rate of PPT Cleavage and Plus-Strand DNA Synthesis
... plus-strand DNA transfers, ...plus-strand DNA priming and plus-strand DNA transfer were also deter- mined for vectors with the mutated PPTs in both the 5 ⬘ and 3 ⬘ ... See full document
5
Role of the 3′ tRNA-Like Structure in Tobacco Mosaic Virus Minus-Strand RNA Synthesis by the Viral RNA-Dependent RNA Polymerase In Vitro
... plus-strand RNA are required for replication in vivo (9, 43, ...(Fig. 1). Mutational analysis of domain D3 has shown that double-helical segment I (closest to the TLS) is essential, and ... See full document
10
RNA sequences controlling the initiation and transfer of duck hepatitis B virus minus-strand DNA.
... nascent minus-strand DNA need not be hybridized to the ...case, RNA-protein interactions would probably contribute greatly to the transfer reaction, since 2-nt DNA-RNA hybrids ... See full document
9
Genetic analysis of the nsP3 region of Sindbis virus: evidence for roles in minus-strand and subgenomic RNA synthesis.
... These data showed that increased replication of the CR3.36 and CR3.39 genomes occurred during complementation and indicate that the ts defect is most likely a defect in nsP3 or nsP3-cont[r] ... See full document
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