[PDF] Top 20 In Vitro CRISPR/Cas9 System for Efficient Targeted DNA Editing
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In Vitro CRISPR/Cas9 System for Efficient Targeted DNA Editing
... in DNA fragments generated by Cas9 complexed with sgRNAS1/ sgRNAS4, sgRNAS2/sgRNAS3, and sgRNAS2/sgRNAS4, respec- tively, were further treated by T4 DNA polymerase, and the prod- ucts were ... See full document
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Development of a CRISPR-Cas9 System for Efficient Genome Editing of Candida lusitaniae
... optimized CRISPR system for C. lusitaniae. A transient CRISPR-Cas9 system was developed for ...for targeted deletion of the ADE2 gene in haploid and diploid ...This system ... See full document
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A CRISPR Assisted Nonhomologous End Joining Strategy for Efficient Genome Editing in Mycobacterium tuberculosis
... including CRISPR–CRISPR-associated protein 9 (Cas9) and CRISPR- Cas12a (Cpf1), have been widely used as genome-editing tools (12, ...The CRISPR-Cas9 system ... See full document
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Review Article Status and contribution of CRISPR/Cas9 based gene-editing system in the development of a low-immunogenic wheat variety
... using CRISPR/Cas9 ribonucleoproteins ...mediated CRISPR/Cas9 method has less chance of off-target mutations in wheat cell in comparison to conventional CRISPR/Cas9 technique as ... See full document
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CRISPR Cas9 Mediated Genome Editing in Leishmania donovani
... prokaryotic CRISPR (clustered regularly interspaced short palindromic repeat)-Cas9, an RNA-guided endonu- clease, has been shown to mediate efficient genome editing in a wide variety of ...the ... See full document
14
A highly efficient ligation-independent cloning system for CRISPR/Cas9 based genome editing in plants
... We conducted proof-of-concept experiments for the paired nickase strategy targeting an endogenous N. benthamiana gene (NbPDS, phytoene desaturase), a transgene (a membrane localized chimeric “aquaporin PIP2-1-mCherry” ... See full document
13
Advances in engineering CRISPR-Cas9 as a molecular Swiss Army knife
... modified Cas9-based CRISPR systems for genome editing tasks, and the main approaches used to engineer these ...of Cas9 domains, redesign of the gRNA, fusion of additional enzymatic domains to ... See full document
31
Targeted Editing of the StPDS Gene using the CRISPR/Cas9 system in Tetraploid Potato
... site-directed editing uses sequence-specific nucleases (SSNs) to cleave DNA double strands at the target site of the genome, resulting in DNA double-strand breaks (DSBs) that will be repaired through ... See full document
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Generation of hyperlipidemic rabbit models using multiple sgRNAs targeted CRISPR/Cas9 gene editing system
... become a routine procedure for functional analysis of gen- etic pathways, both in cellular culture and in animal models. However, most mutations reported are small in- sertions and deletions or point mutations. Now, it ... See full document
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Efficient and Scalable Precision Genome Editing in Staphylococcus aureus through Conditional Recombineering and CRISPR/Cas9 Mediated Counterselection
... two-plasmid system for conditional recombineering and coun- ...two-vector system to carry out conditional recombineering and CRISPR/Cas9-mediated counterselection in ...and Cas9. ... See full document
16
Optimizing CRISPR Cas9 Genome Editing System: A Review
... derived Cas9 protein based immunity system stands amongst others as the most efficient and the most potent gene, editing technology with its diverse application in wide range of biologic ... See full document
15
Highly Specific and Efficient CRISPR/Cas9-Catalyzed Homology-Directed Repair in Drosophila
... pyogenes system was simplified to two components: the Cas9 nuclease and a single chimeric guide RNA ...direct Cas9-mediated cleavage of both DNA strands within the target sequence (Jinek et ... See full document
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Efficient gene editing in Corynebacterium glutamicum using the CRISPR/Cas9 system
... the CRISPR/Cas9 system provides a simple, sequence-specific platform for genome engineering [14, ...II CRISPR/Cas9 system, which requires a mature CRISPR RNA (crRNA), a ... See full document
13
Genome editing of Clostridium autoethanogenum using CRISPR/Cas9
... the CRISPR/Cas9 tool in ...the CRISPR/Cas9 system requires the controlled expres- sion of cas9 and the constitutive expression of sgRNA in the presence of DNA ... See full document
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Efficient CRISPR/Cas9 genome editing with low off target effects in zebrafish
... the CRISPR sequence (supplementary material Fig. S1A) that guides the Cas9 nuclease to the desired genomic locus ...in vitro transcription directly from synthesized oligonucleotides (C13H9orf72), PCR ... See full document
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Upgrading of efficient and scalable CRISPR–Cas-mediated technology for genetic engineering in thermophilic fungus Myceliophthora thermophila
... Cas12a-based CRISPR system for efficient multiplex genome editing, using a single-array approach in ...These CRISPR–Cas12a cassettes worked well for simultaneous multiple gene ...the ... See full document
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Review: Plant Genome Editing Targeted by RNA Guided DNA Endonuclease CRISPR/Cas9
... of CRISPR/Cas9 reac- ...target DNA recognition of crRNA ...guide Cas9-mediated DNA cleavage ...target DNA/crRNA complex structure was stabilized by tracrRNA via 20 base-pairs ... See full document
15
Efficient Editing of Malaria Parasite Genome Using the CRISPR/Cas9 System
... the CRISPR/sgRNA-transfected parasites (see ...material). Targeted deletions of Pysera2 coding region ...using DNA sequencing from transfected parasite mixtures FIG 3 Tagging of the endogenous ... See full document
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Efficient CRISPR/Cas9-Mediated Genome Editing in Mice by Zygote Electroporation of Nuclease
... identify CRISPR/Cas9-mediated NHEJ and HDR mutations, we digested the 466-bp PCR product encompassing the target site with EcoRV and with EcoRI independently (Figure ...Using Cas9 mRNA at 400 ng/ml, ... See full document
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RNA-guided CRISPR-Cas technologies for genome-scale investigation of disease processes
... the targeted region at 3–5 predicted potential off-target genomic regions for each of 12 differ- ent ...the targeted regions for all 12 sgRNAs was verified with only 3 sgRNAs showing appreciable ... See full document
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