with Regard to Physiological Attributes
6.5 Other Physiological Parameters
Nano zerovalent iron (nZVI) triggered high plasma membrane H+-ATPase activity in Arabidopsis, which resulted in a decrease in apoplastic pH, increase in leaf area, and wider stomatal aperture. Gene expression analysis revealedfivefold higher levels of
H+-ATPase isoform responsible for stomatal opening, AHA2, in plants exposed to nZVI. The researchers demonstrated for thefirst time that nZVI enhances stomatal opening by inducing the activation of plasma membrane H+-ATPase, leading to the possibility of increased CO2uptake (Kim et al.2015). TiO2-NPs’ impact on wheat, rapeseed, and Arabidopsis evapotranspiration was evaluated (Larue et al. 2011).
Similarly, TiO2NPs with diameters ranging from 14 to 655 nm did not impact wheat seed germination, biomass, and transpiration (Larue et al.2012). The SiO2NPs apart from deleteriously affecting the plant height and shoot and root biomass also affected the contents of Cu, Mg in shoots, and Na in roots of transgenic cotton (Le et al.2014).
Nutritional analysis of pods from soybean plants cultivated in farm soil amended with CeO2NPs revealed that NPs at 1000 mg kg−1had significantly less Ca but more
Fig. 6.3 aEffect of nano-anatase TiO2on growth of spinach. a Cultured by Hoagland solution.
b Cultured by N-deficient Hoagland solution (Yang et al.2007). b Conceptual model on how OH radical-induced cell wall loosening might alter endocytosis in root cells. Endocytosis (red) of the nZVI-treated seedlings (right) is enhanced compared to that of the control (left), and exocytosis (blue) is vice versa (Adopted from Kim et al.2014)
P and Cu, while pods from 100 mg kg−1nZnO had more Zn, Mn, and Cu. ZnO NP-treated plants shared significant correlations among Zn, P, and S in pods with Zn in roots. The data suggested the evident role of CeO2NPs and ZnO NPs in altering the nutritional value of soybean (Peralta-Videa et al. 2014). In a similar experiment, CeO2NPs effects on the nutritional aspects such as mineral, fatty acid, and amino acid content of wheat were studied. CeO2NPs modified S and Mn storage in grains and modified the amino acid composition and increased linolenic acid by up to 6.17 %.
The linoleic acid content, however, was decreased by up to 1.63 %, compared to the other treatments (Rico et al.2014). Khodakovskaya et al. (2012) demonstrated the ability of MWCNTs to enhance tobacco cell culture growth by upregulating the genes for cell divisions (CycB), cell wall formation (NtLRX1), and water transport (aqua-porin, NNtPIP1). Lahiani et al. (2013) studied the MWCNTs-regulated gene expression for several water channel proteins in soybean, maize, and barley seed coats. Kumar et al. (2013) reported Au NPs have a significant role in altering microRNAs expression levels, which regulate various morphological, physiological, and metabolic processes in plants. Syu et al. (2014) documented the Ag NP-induced gene expressions such as indoleacetic acid protein 8 (IAA8), dehydration-responsive RD22 and 9-cis-epoxycarotenoid dioxygenase (NCED3), involved in various cellular events. Also, Ag NPs were found to negatively influence expression of ACC oxidase 2 and ACC synthase 7, underlining their role as inhibitors of ethylene perception and subsequently interfering with the ethylene biosynthesis in Arabidopsis seedlings (Fig.6.3).
6.6 Discussion
As discussed earlier, nanotechnology is the only recent technological breakthrough that is revolutionizing everyfield it is introduced to. With trendsetting models in the electronics industry and unprecedented strides in medical arena, it seems to be only a matter of time when this technology breaks the bounds in the agro-industry. The major restricting force is the limited understanding available on the primary impacts of this technology on plants as such. Although numerous scientific articles have been published on NP–plant interactions, most revolving around the toxicological aspects of NPs, knowledge of their complex relationship with the crucial physio-logical and biochemical processes impacting a plant system is relatively novice.
Plants in their native environment, domesticated or wild, face a number of chal-lenges to survive. Majority of these are environmental factors such as water shortage, nutrient deficiency, alkalinity/acidity of soil, pollution, insects, and pests all of which encompass the biotic and abiotic agents influencing a plants growth and survival (Fig.6.4).
This chapter touched on the various major industrially relevant NPs currently under study for various different applications. The primary reason for initiating such studies, NP–plant interaction, was to assess the impact of the accidental release of such NPs into the environment and their potential toxicity to the environmental components including plants. Although much data suggested the toxicity of the NPs, evidenced by either germination inhibition or hindering in the growth and development, surprisingly in some cases, the effects were reverse, with noticeable enhancement of growth characteristics. Of course, the effects did vary according to the type, morphological, and chemical characteristics of the NPs and the type of plants being used (Fig.6.5).
Initial studies were limited to basic germination and developmental features, which to date remains constant. Very few studies went beyond and analyzed the molecular and biochemical aspects of the effects. Therefore, it still remains an elusive task to concretely link a particular NPs’ toxicity/beneficial attribute to a specific trait of the NP or the plant as similar NPs can show different effects on similar plant species in different experimental sets.
Fig. 6.4 aEffects of nano-anatase TiO2on the enzyme activities of NH4+assimilation of spinach (Yang et al.2006). b Total soluble protein, chlorophyll content, and P concentration in 6-week-old cluster bean plants (Raliya and Tarafdar2013). The effects of cerium oxide (CeO2) and indium oxide (In2O3) nanoparticles (NPs) exposure on Arabidopsis thaliana (L.) Heynh. were investigated and found to influence both the physiological and molecular level parameters (Adopted from Ma et al.2013)
6.7 Conclusion
The chapter discloses the use of some of the most important and commercially established NPs on the physiological parameters of various plant subjects. It is clear that the NPs exhibit both beneficial and negative influences. Understanding the chemical and physical processes of plants associated with their growth and development is critical in evaluating the role of NPs in either enhancing or retarding these features. From molecular interactions involved in photosynthesis diffusion of water, minerals, and nutrients to plant development, seasonality, and reproduction need to be thoroughly analyzed prior and post NP application. More comprehensive research needs to be performed to expand knowledge on the alterations induced by NPs on the physiological, biochemical, and molecular mechanisms of plants.
Long-term studies need to be designed to assess the NPs role in regulating the physiological processes in plants to construct a database that would be helpful for current and future researchers to progress in the direction of setting a global nano-agro database accessible and useful for all.
Fig. 6.5 There are multifarious determining factors while studying the effects of nanoparticles on plant system, which have to be cumulatively taken into consideration for a comprehensive understanding of the plant–nanoparticle interactions
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