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Ubiquitin-Specific Peptidase 20 Targets TRAF6 and Human T Cell Leukemia Virus Type 1 Tax To Negatively Regulate NF-κB Signaling

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FIG. 1. Expression of USP20 suppresses HTLV-1 Tax- and IL-1�Tax-expressing plasmid, Hpx-Tax (0.05pcDNA3-USP20 (0.518)
FIG. 3. TRAF6 interacts with USP20 and USP33. (A) HEK293T cells were transfected with the indicated plasmids, and immunoprecipitationwas done with either anti-Flag or anti-HA antibody as indicated
FIG. 4. IL-1�Flag-TRAF6, HA-Ub, and USP20. Cells without IL-1to immunoprecipitation (IP) followed by immunoblotting (IB) to detect ubiquitinated TRAF6 (anti-HA; top panel)
FIG. 6. USP20 is frequently expressed at a reduced level in HTLV-1-transformed cells. (A) USP20 transcripts in the indicated T cell lines

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