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Discovery and characterization of the tubercidin biosynthetic pathway from Streptomyces tubercidicus NBRC 13090

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Figure

Fig. 1 Structures of TBN and related antibiotics and the confirmed biosynthetic pathway to  PreQpurine nucleoside antibiotics
Fig. 2 Genetic organization and validation of the gene cluster (tub) for TBN biosynthesis
Table 1 Deduced functions of the open reading frames in the tub gene cluster
Fig. 3 Biochemical characterization of TubE as a CDG‑PRPP 177.9827, 194.9009, 292.0454, and 309.9970, which are fully consistent with the theoretical fragmentation pattern of dephosphorylated presence of PRPP and CDG
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