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Genome-scale metabolic network guided engineering of Streptomyces tsukubaensis for FK506 production improvement

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Academic year: 2020

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Figure

Figure 1 Structures and biosynthetic gene cluster of FK506 and byproducts (FK520, FK506D) in S
Figure 2 Schematic representation of metabolic pathways for S. tsukubaensis. The predicted targets for improved production are shown ingreen (amplification) and red (knockout)
Figure 3 Single gene knockout, overexpression and knockout-overexpression combinations target identification intcsBpeptide synthetase;synthase;heptulosonate-7-phosphate synthase;overexpression on the specific FK506 production and S
Figure 4 The effect of single gene knockout andcomplementation on FK506 production and cell growth
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