16S rDNA gene sequencing
Bacterial Identification for Publication: When Is Enough Enough?
... mid-1990s, sequencing of the small subunit (16S) rDNA genes had be- come commonplace, considered a standard tool of microbial taxonomists not only for elucidating phylogenetic relatedness but also as ...
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Infectious endocarditis caused by Helcococcus kunzii in a vascular patient: a case report and literature review
... -Vitek2 system, bioMérieux, Marcy l’Etoile, France -Matrix-assisted laser desorption time-of-flight mass spectrometry MALDI-TOF MS; Vitek MS bioMérieux -16S rDNA gene sequencing.. Sridha[r] ...
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High-throughput sequencing of <em>16S rDNA</em> amplicons characterizes bacterial composition in cerebrospinal fluid samples from patients with purulent meningitis
... of 16S rDNA gene in these 26 samples of PM were sequenced using the 454 GS FLX ...high-throughput sequencing approach facilitates the characterization of the pathogens in cerebrospinal fluid ...
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Comparing 16S rDNA amplicon sequencing and hybridization capture for pea aphid microbiota diversity analysis
... amplicon sequencing and gene capture to describe quantitatively and qualitatively the microbial diversity of an insect ...conditions, gene capture might be more robust ...the 16S gene ...
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Applicability of PCR DGGE and 16S rDNA Sequencing for Microbiological Analysis of Otitis Media with Effusion
... The PCR was performed in a reaction volume of 50 µl containing 10 mM deoxyribonucleotide triphosphate each, 1.25 U Taq polymerase (Invitrogen, USA), 10x reaction buffer, 10 µmol of the each primer and 200 ng (1 µl) of ...
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High-throughput sequencing of <em>16S rDNA</em> amplicons characterizes bacterial composition in bronchoalveolar lavage fluid in patients with ventilator-associated pneumonia
... high-throughput sequencing approach and analyze the species richness and complexity via alpha and beta diversity ...of 16S rDNA gene in these 27 samples of VAP were sequenced using the 454 GS ...
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Evaluation of Partial 16S Ribosomal DNA Sequencing for Identification of Nocardia Species by Using the MicroSeq 500 System with an Expanded Database
... partial 16S ribosomal DNA (rDNA) sequencing by the MicroSeq 500 system with an expanded ...ⴕ 16S rDNA sequences derived from 28 reference strains (from the American Type Culture ...
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Usefulness of the MicroSeq 500 16S Ribosomal DNA Based Bacterial Identification System for Identification of Clinically Significant Bacterial Isolates with Ambiguous Biochemical Profiles
... and sequencing procedures, the use of 16S rRNA gene sequence-based methods in clinical microbiology laboratories is largely limited to identification of strains that are difficult to identify by ...
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Two different 16S rRNA genes in a mycobacterial strain
... the 16S rDNA of Mycobacterium strain ...encoding 16S rRNA, molecules obtained by PCR amplification (positions 9 to 1039) were cloned separately into the PCR II plasmid (Invitrogen, NV Leek, The ...
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Characterization of a spotted fever group Rickettsia from Ixodes ricinus ticks in Sweden
... nucleotide sequencing, the present Rickettsia ...by 16S ribosomal DNA (rDNA) sequencing and restriction fragment length polymorphism ...The 16S rDNA sequencing resulted in ...
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Isolation and Characterization of a Black PigmentedCorynebacterium sp from a Woman with Spontaneous Abortion
... FIG. 2. Evolutionary distance dendrogram of selected corynebacterial 16S rRNA sequences, including that of Corynebacterium sp. strain CN-1. M. tuberculosis (X55588) and R. dentocariosa (M59055) were chosen as ...
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Development and Evaluation of a Quality Controlled Ribosomal Sequence Database for 16S Ribosomal DNA Based Identification of Staphylococcus Species
... ribosomal gene sequence database as part of the Ribosomal Differentiation of Microorganisms (RIDOM) project and to overcome the drawbacks of phenotypic identification systems and publicly accessible sequence ...
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Streptococcus sinensis sp nov , a Novel Species Isolated from a Patient with Infective Endocarditis
... differences from HKU4 have been detected in the aortic valve of a patient with infective endocarditis (GenBank accession no. AY049738). This shows that S. sinensis is probably not one of those bacteria that are isolated ...
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PURIFICATION OF Β-KETOTHIOLASE FROM HALOPHILIC ARCHAEA HALOARCULA SP.1 ISOLATED FROM BHAVNAGAR COAST, GUJARAT, INDIA.
... of 16S rDNA partial sequencing (642 bp) of the strain NPW-9 showed maximum sequence identity (100%) with the complete sequence of Haloarcula ...
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Potential contribution of the uterine microbiome in the development of endometrial cancer
... Methods: We undertook a study of the microbiome in samples taken from different locations along the female reproductive tract in patients with endometrial cancer (n = 17), patients with endometrial hyperplasia ...
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Detection of bacterial DNA from central venous catheter removed from patients by next generation sequencing: a preliminary clinical study
... Sanger sequencing of 16S rDNA, amplified using a uni- versal primer set, can be applied when the specimen contains only one species, but it is usually impossible to simultaneously identify more than ...
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Complete genome sequence of the thermophilic, hydrogen-oxidizing Bacillus tusciae type strain (T2T) and reclassification in the new genus, Kyrpidia gen. nov. as Kyrpidia tusciae comb. nov. and emendation of the family Alicyclobacillaceae da Costa and Rainey, 2010
... a novel species within the genus Bacillus [1]. While B. schlegelii was named by the same senior author as B. tusciae and most probably is as mis- placed in the genus Bacillus as B. tusciae (see be- low), B. ...
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First report of Wolbachia in Damaeus onustus (Acari: Oribatida)
... 710-bp 16S rDNA sequence detected in the oribatid mite with the sequences deposited in GenBank revealed its 92 – 93% similarity to the 16S rDNA sequences of Wolbachia identified in some ...
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Phenotypic and Genotypic Characterization of Clinical Strains of CDC Group IVc 2
... The 16S rRNA gene was PCR amplified with two universal primers, 5F and 1540R (PE Applied Biosystems, ...the 16S rRNA gene, ...MicroSeq 16S rRNA gene kit (ABD, Foster City, ...
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Unraveling the diversity of sedimentary sulfate-reducing prokaryotes (SRP) across Tibetan saline lakes using epicPCR
... by sequencing of 16S rRNA gene PCR amplicons and epicPCR products of fused 16S rRNA plus dsrB gene, respectively, with Proteobacteria , Firmicutes, and Bacteroidetes being the dominant ...
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