18S rRNA
Specific detection of fungal pathogens by 18S rRNA gene PCR in microbial keratitis
... The most common initial laboratory procedure done in diagnosis of fungal keratitis is microscopic examination. In this study, a positive microscopic examination was observed in 30% (9) of patients. The percentage of ...
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Applied Genomics: Data Mining Reveals Species Specific Malaria Diagnostic Targets More Sensitive than 18S rRNA
... Accurate and rapid diagnosis of malaria infections is crucial for implementing species-appropriate treat- ment and saving lives. Molecular diagnostic tools are the most accurate and sensitive method of detecting ...
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The HIV 1 Nef Protein Interacts with two components of the 40S small ribosomal subunit, the RPS10 protein and the 18S rRNA
... of 18S pre-rRNA in part directed by ...of 18S pre-rRNA maturation at cleavage sites A0/1 or at site ...the rRNA and ribosomal proteins can be coupled, ...less rRNA synthesized ...
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Use of 18S rRNA Gene Based PCR Assay for Diagnosis of Acanthamoeba Keratitis in Non Contact Lens Wearers in India
... An 18S rRNA gene-based PCR test, highly specific for the genus Acanthamoeba, has recently been reported in the molecular diagnosis of Acanthamoeba ...
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Feistel, Susanne (2012): Molekulare Morphologie der 18S rRNA der Bivalvia. Dissertation, LMU München: Fakultät für Biologie
... der 18S rRNA der Bivalvia angewendet wurde, ist nach ihrem grundlegenden Prinzip eine logische und viel versprechende Methode, um sehr alte Verwandtschaftsbeziehungen innerhalb eines Taxons ...
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Multiplex, DNase free one step reverse transcription PCR for Plasmodium 18S rRNA and spliced gametocyte specific mRNAs
... Plasmodium 18S rRNA ...Plasmodium 18S rRNA RT-PCR was more sensitive than other spliced mRNA targets for one-step RT-PCR gametocyte ...Plasmodium 18S rRNA for direct one-step ...
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Molecular analysis of 18S rRNA gene of Cryptosporidium parasites from patients living in Iran, Malawi, Nigeria and Vietnam
... There have been few reports on the molecular analysis of the various Cryptosporidium species in diarrheic or non diarrheic individuals in Iran. Also, there are limited or no molecular studies of cryptosporidiosis in many ...
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Evaluating 18s rRNA LAMP and selective whole genome amplification (sWGA) assay in detecting asymptomatic Plasmodium falciparum infections in blood donors
... The LAMP primers used in this study were designed by Poon et al. [26]. The LAMP assay detects the 18S rRNA target in human Plasmodium parasites. In brief, the LAMP reaction volumes were made up of 12.5 μL ...
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The Triticum Mosaic Virus Internal Ribosome Entry Site Relies on a Picornavirus-Like YX-AUG Motif To Designate the Preferred Translation Initiation Site and To Likely Target the 18S rRNA
... the 18S rRNA, a structural RNA component of the 40S ribosomal subunit, at nt 1131 to 1137 ...Similar 18S rRNA binding sites have been predicted in other plant viral cap-independent translation ...
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PCR-Denaturing gradient gel electrophoresis profiling of inter- and intraspecies 18S rRNA gene sequence heterogeneity is an accurate and sensitive method to assess species diversity of arbuscular mycorrhizal fungi of the genus Gigaspora
... the 18S rRNA genes of the genus Gigaspora to assess the use of this marker for the discrimination of Gigaspora isolates and of Gigasporaceae populations from environmental ...the 18S rRNA gene ...
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Using PCR Amplification and Genetic Sequence Analysis of 18S rRNA Genes to Survey the Microbial Diversity and Distribution of Eukaryotic Microbes Inhabiting Two Thermo-acidic Streams in Yellowstone National Park, Wyoming
... cloned 18S rRNA genes, suggest that this single species is the predominant alga in the Nymph Creek mat at temperatures above ...same 18S rRNA gene sequence as the AAC1 sequences cloned ...
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A Clinicomycological study of Onychomycosis, its Antifungal Susceptibility Pattern and Rapid Detection of Trichophyton Genus from Nail Samples by PCR Analysis of 18S rRNA Gene Internal Transcribed Spacer Region.
... CLINICOMYCOLOGICAL STUDY OF ONYCHOMYCOSIS, ITS ANTIFUNGAL SUSCEPTIBILITY PATTERN AND RAPID DETECTION OF TRICHOPHYTON GENUS FROM NAIL SAMPLES BY PCR ANALYSIS OF 18S rRNA GENE INTERNAL TRANSCRIBED SPACER ...
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Development of a Highly Sensitive Genus Specific Quantitative Reverse Transcriptase Real Time PCR Assay for Detection and Quantitation of Plasmodium by Amplifying RNA and DNA of the 18S rRNA Genes
... on 18S rRNA sequences deposited in GenBank and were designed using the Web-based soft- ware Primer3 ...of rRNA distributed in all the chromosomes: 1, 5, 7, 11, and ...Plasmodium 18S ...
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Detection of Four Plasmodium Species in Blood from Humans by 18S rRNA Gene Subunit Based and Species Specific Real Time PCR Assays
... the 18S rRNA gene of the genus Plasmodium was designed; the primer set was polymorphic enough internally to design four species- specific probes for ...the 18S screening ...between 18S ...
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Molecular Analysis of the 18S rRNA Gene of Cryptosporidium Parasites from Patients with or without Human Immunodeficiency Virus Infections Living in Kenya, Malawi, Brazil, the United Kingdom, and Vietnam
... So far, a limited number of isolates have been typed from developing countries and especially from human immunodefi- ciency virus (HIV)-infected people (5, 12, 16, 17, 19, 20, 24), yet cryptosporidiosis is endemic in ...
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Molecular detection of 18s rrna gene of candida albicans isolated from pregnant women with clinical diagnosis of vulvovaginitis
... This study has demonstrated the efficacy of the 18S rRNA gene to detect C. albicans in clinical samples. This PCR based test is a rapid, sensitive and a useful method to detect cadidial etiology in the ...
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Plasmodium 18S rRNA of intravenously administered sporozoites does not persist in peripheral blood
... zoite 18S rRNA does not persistently circulate in periph- eral blood and suggests that timing and concentration of the pre-erythrocytic 18S rDNA positivity 24 h post- challenge in the Abkallo study ...
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High resolution melting analysis of the 18S rRNA gene for the rapid diagnosis of bovine babesiosis
... Blood samples comprising both field-collected samples and samples from experimentally infected cattle were used to evaluate and compare HRM with the identification results using nested PCR and 18S rRNA gene ...
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PHYLOGENETIC ANALYSIS OF FRESHWATER MUSSEL BY Corbicula regularis 18S rRNA GENE SEQUENCING
... The amplified PCR product was partially sequenced and the gene sequences were analyzed against the DNA database of Japan (DDBJ) database using BLAST N (nucleotide- nucleotide BLAST) program packages (Zhang et al., 2000) ...
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Fluorescent Oligonucleotide Probes for Clinical and Environmental Detection of Acanthamoeba and the T4 18S rRNA Gene Sequence Type
... Use of ST4P with trophozoites. The specificity of ST4P was tested by using cells doubly stained with GSP and ST4P. Amoebae from each of the 12 described 18S rRNA gene (rDNA) sequence types of Acanthamoeba ...
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